LOCUS       sequence-81366-e       16310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP and Synaptophysin-TagRFP-T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16310)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 16310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-EGFP-N-P-M-SynPhRFP-L
FEATURES             Location/Qualifiers
     source          1..16310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1452..1461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1458..1460,1461..1463,1464..2177)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1458..1460,1461..1463,1464..2174)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1503..1524)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1764..1783)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2111..2132
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      5523..5532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6483..6492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6489..7220
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             8972..8983
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13982..14000)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13996..14043
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(14066..14082)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14066..14082)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(14119..14139)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14119..14137)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14158..14174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14158..14174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14171..14193)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14182..14198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14206..14212,14213..14230,14231..14236))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14251..14272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14389..14406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14560..15148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14640..14659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15319..16110,16111..16179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15942..15961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16180..16284)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 ccgggaaagc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1501 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1561 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1621 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1681 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1741 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1801 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1861 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     1921 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     1981 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2041 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2101 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2161 acgagctgta caagtaagct agggaagttg aataacaaaa tgccggaaat ctacggattg
     2221 tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca aacatggatg
     2281 ccgacaagat tgtattcaaa gtcaataatc aggtggtctc tttgaagcct gagattatcg
     2341 tggatcaata tgagtacaag taccctgcca tcaaagattt gaaaaagccc tgtataactc
     2401 taggaaaggc tcccgattta aataaagcat acaagtcagt tttgtcaggc atgagcgccg
     2461 ccaaacttaa tcctgacgat gtatgttcct atttggcagc ggcaatgcag ttttttgagg
     2521 ggacatgtcc ggaagactgg accagctatg gaattgtgat tgcacgaaaa ggagataaga
     2581 tcaccccagg ttctctggtg gagataaaac gtactgatgt agaagggaat tgggctctga
     2641 caggaggcat ggaactgaca agagacccca ctgtccctga gcatgcgtcc ttagtcggtc
     2701 ttctcttgag tctgtatagg ttgagcaaaa tatccgggca aaacactggt aactataaga
     2761 caaacattgc agacaggata gagcagattt ttgagacagc cccttttgtt aaaatcgtgg
     2821 aacaccatac tctaatgaca actcacaaaa tgtgtgctaa ttggagtact ataccaaact
     2881 tcagattttt ggccggaacc tatgacatgt ttttctcccg gattgagcat ctatattcag
     2941 caatcagagt gggcacagtt gtcactgctt atgaagactg ttcaggactg gtatcattta
     3001 ctgggttcat aaaacaaatc aatctcaccg ctagagaggc aatactatat ttcttccaca
     3061 agaactttga ggaagagata agaagaatgt ttgagccagg gcaggagaca gctgttcctc
     3121 actcttattt catccacttc cgttcactag gcttgagtgg gaaatctcct tattcatcaa
     3181 atgctgttgg tcacgtgttc aatctcattc actttgtagg atgctatatg ggtcaagtca
     3241 gatccctaaa tgcaacggtt attgctgcat gtgctcctca tgaaatgtct gttctagggg
     3301 gctatctggg agaggaattc ttcgggaaag ggacatttga aagaagattc ttcagagatg
     3361 agaaagaact tcaagaatac gaggcggctg aactgacaaa gactgacgta gcactggcag
     3421 atgatggaac tgtcaactct gacgacgagg actacttttc aggtgaaacc agaagtccgg
     3481 aggctgttta tactcgaatc atgatgaatg gaggtcgact aaagagatct cacatacgga
     3541 gatatgtctc agtcagttcc aatcatcaag cccgtccaaa ctcattcgcc gagtttctaa
     3601 acaagacata ttcgagtgac tcataagaag ttgaataaca aaatgccgga aatctacgga
     3661 ttgtgtatat ccatcatgaa aaaaactaac acccctcctt tcgaaccatc ccaaacatga
     3721 gcaagatctt tgtcaatcct agtgctatta gagccggtct ggccgatctt gagatggctg
     3781 aagaaactgt tgatctgatc aatagaaata tcgaagacaa tcaggctcat ctccaagggg
     3841 aacccataga ggtggacaat ctccctgagg atatggggcg acttcacctg gatgatggaa
     3901 aatcgcccaa ccatggtgag atagccaagg tgggagaagg caagtatcga gaggactttc
     3961 agatggatga aggagaggat cctagcttcc tgttccagtc atacctggaa aatgttggag
     4021 tccaaatagt cagacaaatg aggtcaggag agagatttct caagatatgg tcacagaccg
     4081 tagaagagat tatatcctat gtcgcggtca actttcccaa ccctccagga aagtcttcag
     4141 aggataaatc aacccagact actggccgag agctcaagaa ggagacaaca cccactcctt
     4201 ctcagagaga aagccaatca tcgaaagcca ggatggcggc tcaaattgct tctggccctc
     4261 cagcccttga atggtcggct accaatgaag aggatgatct atcagtggag gctgagatcg
     4321 ctcaccagat tgcagaaagt ttctccaaaa aatataagtt tccctctcga tcctcaggga
     4381 tactcttgta taattttgag caattgaaaa tgaaccttga tgatatagtt aaagaggcaa
     4441 aaaatgtacc aggtgtgacc cgtttagccc atgacgggtc caaactcccc ctaagatgtg
     4501 tactgggatg ggtcgctttg gccaactcta agaaattcca gttgttagtc gaatccgaca
     4561 agctgagtaa aatcatgcaa gatgacttga atcgctatac atcttgctaa ccgaacctct
     4621 cccctcagtc cctctagaca ataaaatccg agatgtccca aagtcaacat gaaaaaaaca
     4681 ggcaacacca ctgataaaat gaacctccta cgtaagatag tgaaaaaccg cagggacgag
     4741 gacactcaaa aatcctctcc cgcgtcagcc cctctggatg acgatgactt gtggcttcca
     4801 ccccctgaat acgtcccgct gaaagaactt acaggcaaga agaacatgag gaacttttgt
     4861 atcaacggaa gggttaaagt gtgtagcccg aatggttact cgttcaggat cctgcggcac
     4921 attctgaaat cattcgacga gatatattct gggaatcata ggatgatcgg gttagtcaaa
     4981 gtggttattg gactggcttt gtcaggatct ccagtccctg agggcctgaa ctgggtatac
     5041 aaattgagga gaacctttat cttccagtgg gctgattcca ggggccctct tgaaggggag
     5101 gagttggaat actctcagga gatcacttgg gatgatgata ctgagttcgt cggattgcaa
     5161 ataagagtga ttgcaaaaca gtgtcatatc cagggcagag tctggtgtat caacatgaac
     5221 ccgagagcat gtcaactatg gtctgacatg tctcttcaga cacaaaggtc cgaagaggac
     5281 aaagattcct ctctgcttct agaataatca gattatatcc cgcaaattta tcacttgttt
     5341 acctctggag gagagaacat atgggctcaa ctccaaccct tgggagcaat ataacaaaaa
     5401 acatgttatg gtgccattaa accgctgcat ttcatcaaag tcaagttgat tacctttaca
     5461 ttttgatcct cttggatgtg aaaaaaacta ttaacatccc tcaaaagacc ccgggtacgg
     5521 ccgccaccat ggacgtggtg aatcagctgg tggctggggg tcagttccgg gtggtcaagg
     5581 agccccttgg cttcgtgaag gtgctgcagt gggtctttgc catcttcgcc tttgctacgt
     5641 gcggcagcta caccggagag cttcggctga gcgtggagtg tgccaacaag acggagagtg
     5701 ccctcaacat cgaagtcgaa tttgagtacc cattcaggct gcaccaagtg tactttgatg
     5761 caccctcctg cgttaaaggg ggcactacca agatcttcct agttggtgac tactcctcct
     5821 cgggtgaatt ctttgtcacc gtggctgtgt ttgccttcct ctactccatg ggggccctgg
     5881 ccacctacat cttcctgcag aacaagtacc gagagaacaa caaagggcca atgatggact
     5941 tcctggccac agcagtgttc gctttcatgt ggctagttag ctcatccgcc tgggccaaag
     6001 gcctgtccga tgtgaagatg gccactgacc cagagaacat tatcaaggag atgcctatgt
     6061 gccgccagac aggaaacaca tgcaaggaac tgagggaccc tgtgacttca ggactcaaca
     6121 cctcggtggt gtttggcttc ctgaacctgg tgctctgggt tggcaaccta tggttcgtgt
     6181 tcaaggagac aggctgggcc gccccattca tgcgcgcacc tccaggcgcc ccagaaaagc
     6241 aaccagctcc tggcgatgcc tacggcgatg cgggctatgg gcagggcccc ggaggctatg
     6301 ggccccagga ctcctacggg cctcagggtg gttatcaacc cgattacggg cagccagcca
     6361 gcggcggtgg cggtggctac gggcctcagg gcgactatgg gcagcaaggc tacggccaac
     6421 agggtgcgcc cacctccttc tccaatcaga tgggcggagg ccctgggatc caccggccgg
     6481 tcgccaccat ggtgtctaag ggcgaagagc tgattaagga gaacatgcac atgaagctgt
     6541 acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc
     6601 cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg
     6661 ccttcgacat cctggctacc agcttcatgt acggcagcag aaccttcatc aaccacaccc
     6721 agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg gagagagtca
     6781 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
     6841 gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc
     6901 agaagaaaac actcggctgg gaggccaaca ccgagatgct gtaccccgct gacggcggcc
     6961 tggaaggcag aaccgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact
     7021 tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact
     7081 atgtggacca cagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc
     7141 acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac aaacttaatg
     7201 gcatggacga gctgtacaag taagctagct tataaagtgc tgggtcatct aagcttttca
     7261 gtcgagaaaa aaacattaga tcagaagaac aactggcaac acttctcaac ctgagactta
     7321 cttcaagatg ctcgatcctg gagaggtcta tgatgaccct attgacccaa tcgagttaga
     7381 ggctgaaccc agaggaaccc ccattgtccc caacatcttg aggaactctg actacaatct
     7441 caactctcct ttgatagaag atcctgctag actaatgtta gaatggttaa aaacagggaa
     7501 tagaccttat cggatgactc taacagacaa ttgctccagg tctttcagag ttttgaaaga
     7561 ttatttcaag aaggtagatt tgggttctct caaggtgggc ggaatggctg cacagtcaat
     7621 gatttctctc tggttatatg gtgcccactc tgaatccaac aggagccgga gatgtataac
     7681 agacttggcc catttctatt ccaagtcgtc ccccatagag aagctgttga atctcacgct
     7741 aggaaataga gggctgagaa tccccccaga gggagtgtta agttgccttg agagggttga
     7801 ttatgataat gcatttggaa ggtatcttgc caacacgtat tcctcttact tgttcttcca
     7861 tgtaatcacc ttatacatga acgccctaga ctgggatgaa gaaaagacca tcctagcatt
     7921 atggaaagat ttaacctcag tggacatcgg gaaggacttg gtaaagttca aagaccaaat
     7981 atggggactg ctgatcgtga caaaggactt tgtttactcc caaagttcca attgtctttt
     8041 tgacagaaac tacacactta tgctaaaaga tcttttcttg tctcgcttca actccttaat
     8101 ggtcttgctc tctcccccag agccccgata ctcagatgac ttgatatctc aactatgcca
     8161 gctgtacatt gctggggatc aagtcttgtc tatgtgtgga aactccggct atgaagtcat
     8221 caaaatattg gagccatatg tcgtgaatag tttagtccag agagcagaaa agtttaggcc
     8281 tctcattcat tccttgggag actttcctgt atttataaaa gacaaggtaa gtcaacttga
     8341 agagacgttc ggtccctgtg caagaaggtt ctttagggct ctggatcaat tcgacaacat
     8401 acatgacttg gtttttgtgt ttggctgtta caggcattgg gggcacccat atatagatta
     8461 tcgaaagggt ctgtcaaaac tatatgatca ggttcacctt aaaaaaatga tagataagtc
     8521 ctaccaggag tgcttagcaa gcgacctagc caggaggatc cttagatggg gttttgataa
     8581 gtactccaag tggtatctgg attcaagatt cctagcccga gaccacccct tgactcctta
     8641 tatcaaaacc caaacatggc cacccaaaca tattgtagac ttggtggggg atacatggca
     8701 caagctcccg atcacgcaga tctttgagat tcctgaatca atggatccgt cagaaatatt
     8761 ggatgacaaa tcacattctt tcaccagaac gagactagct tcttggctgt cagaaaaccg
     8821 aggggggcct gttcctagcg aaaaagttat tatcacggcc ctgtctaagc cgcctgtcaa
     8881 tccccgagag tttctgaggt ctatagacct cggaggattg ccagatgaag acttgataat
     8941 tggcctcaag ccaaaggaac gggaattgaa gattgaaggt cgattctttg ctctaatgtc
     9001 atggaatcta agattgtatt ttgtcatcac tgaaaaactc ttggccaact acatcttgcc
     9061 actttttgac gcgctgacta tgacagacaa cctgaacaag gtgtttaaaa agctgatcga
     9121 cagggtcacc gggcaagggc ttttggacta ttcaagggtc acatatgcat ttcacctgga
     9181 ctatgaaaag tggaacaacc atcaaagatt agagtcaaca gaggatgtat tttctgtcct
     9241 agatcaagtg tttggattga agagagtgtt ttctagaaca cacgagtttt ttcaaaaggc
     9301 ctggatctat tattcagaca gatcagacct catcgggtta cgggaggatc aaatatactg
     9361 cttagatgcg tccaacggcc caacctgttg gaatggccag gatggcgggc tagaaggctt
     9421 acggcagaag ggctggagtc tagtcagctt attgatgata gatagagaat ctcaaatcag
     9481 gaacacaaga accaaaatac tagctcaagg agacaaccag gttttatgtc cgacatacat
     9541 gttgtcgcca gggctatctc aagaggggct cctctatgaa ttggagagaa tatcaaggaa
     9601 tgcactttcg atatacagag ccgtcgagga aggggcatct aagctagggc tgatcatcaa
     9661 gaaagaagag accatgtgta gttatgactt cctcatctat ggaaaaaccc ctttgtttag
     9721 aggtaacata ttggtgcctg agtccaaaag atgggccaga gtctcttgcg tctctaatga
     9781 ccaaatagtc aacctcgcca atataatgtc gacagtgtcc accaatgcgc taacagtggc
     9841 acaacactct caatctttga tcaaaccgat gagggatttt ctgctcatgt cagtacaggc
     9901 agtctttcac tacctgctat ttagcccaat cttaaaggga agagtttaca agattctgag
     9961 cgctgaaggg gagagctttc tcctagccat gtcaaggata atctatctag atccttcttt
    10021 gggagggata tctggaatgt ccctcggaag attccatata cgacagttct cagaccctgt
    10081 ctctgaaggg ttatccttct ggagagagat ctggttaagc tcccaagagt cctggattca
    10141 cgcgttgtgt caagaggctg gaaacccaga tcttggagag agaacactcg agagcttcac
    10201 tcgccttcta gaagatccga ccaccttaaa tatcagagga ggggccagtc ctaccattct
    10261 actcaaggat gcaatcagaa aggctttata tgacgaggtg gacaaggtgg aaaattcaga
    10321 gtttcgagag gcaatcctgt tgtccaagac ccatagagat aattttatac tcttcttaat
    10381 atctgttgag cctctgtttc ctcgatttct cagtgagcta ttcagttcgt cttttttggg
    10441 aatccccgag tcaatcattg gattgataca aaactcccga acgataagaa ggcagtttag
    10501 aaagagtctc tcaaaaactt tagaagaatc cttctacaac tcagagatcc acgggattag
    10561 tcggatgacc cagacacctc agagggttgg gggggtgtgg ccttgctctt cagagagggc
    10621 agatctactt agggagatct cttggggaag aaaagtggta ggcacgacag ttcctcaccc
    10681 ttctgagatg ttgggattac ttcccaagtc ctctatttct tgcacttgtg gagcaacagg
    10741 aggaggcaat cctagagttt ctgtatcagt actcccgtcc tttgatcagt catttttttc
    10801 acgaggcccc ctaaagggat acttgggctc gtccacctct atgtcgaccc agctattcca
    10861 tgcatgggaa aaagtcacta atgttcatgt ggtgaagaga gctctatcgt taaaagaatc
    10921 tataaactgg ttcattacta gagattccaa cttggctcaa gctctaatta ggaacattat
    10981 gtctctgaca ggccctgatt tccctctaga ggaggcccct gtcttcaaaa ggacggggtc
    11041 agccttgcat aggttcaagt ctgccagata cagcgaagga gggtattctt ctgtctgccc
    11101 gaacctcctc tctcatattt ctgttagtac agacaccatg tctgatttga cccaagacgg
    11161 gaagaactac gatttcatgt tccagccatt gatgctttat gcacagacat ggacatcaga
    11221 gctggtacag agagacacaa ggctaagaga ctctacgttt cattggcacc tccgatgcaa
    11281 caggtgtgtg agacccattg acgacgtgac cctggagacc tctcagatct tcgagtttcc
    11341 ggatgtgtcg aaaagaatat ccagaatggt ttctggggct gtgcctcact tccagaggct
    11401 tcccgatatc cgtctgagac caggagattt tgaatctcta agcggtagag aaaagtctca
    11461 ccatatcgga tcagctcagg ggctcttata ctcaatctta gtggcaattc acgactcagg
    11521 atacaatgat ggaaccatct tccctgtcaa catatacggc aaggtttccc ctagagacta
    11581 tttgagaggg ctcgcaaggg gagtattgat aggatcctcg atttgcttct tgacaagaat
    11641 gacaaatatc aatattaata gacctcttga attggtctca ggggtaatct catatattct
    11701 cctgaggcta gataaccatc cctccttgta cataatgctc agagaaccgt ctcttagagg
    11761 agagatattt tctatccctc agaaaatccc cgccgcttat ccaaccacta tgaaagaagg
    11821 caacagatca atcttgtgtt atctccaaca tgtgctacgc tatgagcgag agataatcac
    11881 ggcgtctcca gagaatgact ggctatggat cttttcagac tttagaagtg ccaaaatgac
    11941 gtacctatcc ctcattactt accagtctca tcttctactc cagagggttg agagaaacct
    12001 atctaagagt atgagagata acctgcgaca attgagttct ttgatgaggc aggtgctggg
    12061 cgggcacgga gaagatacct tagagtcaga cgacaacatt caacgactgc taaaagactc
    12121 tttacgaagg acaagatggg tggatcaaga ggtgcgccat gcagctagaa ccatgactgg
    12181 agattacagc cccaacaaga aggtgtcccg taaggtagga tgttcagaat gggtctgctc
    12241 tgctcaacag gttgcagtct ctacctcagc aaacccggcc cctgtctcgg agcttgacat
    12301 aagggccctc tctaagaggt tccagaaccc tttgatctcg ggcttgagag tggttcagtg
    12361 ggcaaccggt gctcattata agcttaagcc tattctagat gatctcaatg ttttcccatc
    12421 tctctgcctt gtagttgggg acgggtcagg ggggatatca agggcagtcc tcaacatgtt
    12481 tccagatgcc aagcttgtgt tcaacagtct tttagaggtg aatgacctga tggcttccgg
    12541 aacacatcca ctgcctcctt cagcaatcat gaggggagga aatgatatcg tctccagagt
    12601 gatagatctt gactcaatct gggaaaaacc gtccgacttg agaaacttgg caacctggaa
    12661 atacttccag tcagtccaaa agcaggtcaa catgtcctat gacctcatta tttgcgatgc
    12721 agaagttact gacattgcat ctatcaaccg gatcaccctg ttaatgtccg attttgcatt
    12781 gtctatagat ggaccactct atttggtctt caaaacttat gggactatgc tagtaaatcc
    12841 aaactacaag gctattcaac acctgtcaag agcgttcccc tcggtcacag ggtttatcac
    12901 ccaagtaact tcgtcttttt catctgagct ctacctccga ttctccaaac gagggaagtt
    12961 tttcagagat gctgagtact tgacctcttc cacccttcga gaaatgagcc ttgtgttatt
    13021 caattgtagc agccccaaga gtgagatgca gagagctcgt tccttgaact atcaggatct
    13081 tgtgagagga tttcctgaag aaatcatatc aaatccttac aatgagatga tcataactct
    13141 gattgacagt gatgtagaat cttttctagt ccacaagatg gttgatgatc ttgagttaca
    13201 gaggggaact ctgtctaaag tggctatcat tatagccatc atgatagttt tctccaacag
    13261 agtcttcaac gtttccaaac ccctaactga cccctcgttc tatccaccgt ctgatcccaa
    13321 aatcctgagg cacttcaaca tatgttgcag tactatgatg tatctatcta ctgctttagg
    13381 tgacgtccct agcttcgcaa gacttcacga cctgtataac agacctataa cttattactt
    13441 cagaaagcaa gtcattcgag ggaacgttta tctatcttgg agttggtcca acgacacctc
    13501 agtgttcaaa agggtagcct gtaattctag cctgagtctg tcatctcact ggatcaggtt
    13561 gatttacaag atagtgaaga ctaccagact cgttggcagc atcaaggatc tatccagaga
    13621 agtggaaaga caccttcata ggtacaacag gtggatcacc ctagaggata tcagatctag
    13681 atcatcccta ctagactaca gttgcctgtg aaccggatac tcctggaagc ctgcccatgc
    13741 taagactctt gtgtgatgta tcttgaaaaa aacaagatcc taaatctgaa cctttggttg
    13801 tttgattgtt tttctcattt ttgttgttta tttgttaagc gtgggtcggc atggcatctc
    13861 cacctcctcg cggtccgacc tgggcatccg aaggaggacg cacgtccact cggatggcta
    13921 agggagggcg gggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
    13981 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
    14041 ttgctgaaag gaggaactat atccggatcc actagttcta gagcggccgc caccgcggtg
    14101 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
    14161 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    14221 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    14281 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    14341 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    14401 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    14461 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    14521 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    14581 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    14641 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    14701 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    14761 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    14821 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    14881 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    14941 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    15001 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    15061 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    15121 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    15181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    15241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    15301 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    15361 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    15421 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    15481 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    15541 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    15601 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    15661 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    15721 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    15781 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    15841 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    15901 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    15961 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    16021 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    16081 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    16141 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    16201 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    16261 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
