LOCUS       sequence-81367-e       15330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15330)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 15330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-mCherry-N-P-M-EGFP-L
FEATURES             Location/Qualifiers
     source          1..15330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1453..1462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1459..2169
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1604..1622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1872..1891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      5517..5526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5523..5525,5526..5528,5529..6242)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5523..5525,5526..5528,5529..6239)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5568..5589)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5829..5848)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6176..6197
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7991..8002
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13002..13020)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13016..13063
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13086..13102)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13086..13102)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13139..13159)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13139..13157)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13178..13194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13178..13194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13191..13213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13202..13218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13226..13232,13233..13250,13251..13256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13271..13292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13409..13426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13580..14168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13660..13679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14339..15130,15131..15199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14962..14981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15200..15304)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 ccggtgaaag ccgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     1501 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1561 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1621 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1681 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1741 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1801 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1861 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1921 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1981 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2041 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2101 atcgtggaac agtacgaacg cgccgagggc cgccactcta ccggcggcat ggacgagctg
     2161 tacaagtaat gacctaggga agttgaataa caaaatgccg gaaatctacg gattgtgtat
     2221 atccatcatg aaaaaaacta acacccctcc tttcgaacca tcccaaacat ggatgccgac
     2281 aagattgtat tcaaagtcaa taatcaggtg gtctctttga agcctgagat tatcgtggat
     2341 caatatgagt acaagtaccc tgccatcaaa gatttgaaaa agccctgtat aactctagga
     2401 aaggctcccg atttaaataa agcatacaag tcagttttgt caggcatgag cgccgccaaa
     2461 cttaatcctg acgatgtatg ttcctatttg gcagcggcaa tgcagttttt tgaggggaca
     2521 tgtccggaag actggaccag ctatggaatt gtgattgcac gaaaaggaga taagatcacc
     2581 ccaggttctc tggtggagat aaaacgtact gatgtagaag ggaattgggc tctgacagga
     2641 ggcatggaac tgacaagaga ccccactgtc cctgagcatg cgtccttagt cggtcttctc
     2701 ttgagtctgt ataggttgag caaaatatcc gggcaaaaca ctggtaacta taagacaaac
     2761 attgcagaca ggatagagca gatttttgag acagcccctt ttgttaaaat cgtggaacac
     2821 catactctaa tgacaactca caaaatgtgt gctaattgga gtactatacc aaacttcaga
     2881 tttttggccg gaacctatga catgtttttc tcccggattg agcatctata ttcagcaatc
     2941 agagtgggca cagttgtcac tgcttatgaa gactgttcag gactggtatc atttactggg
     3001 ttcataaaac aaatcaatct caccgctaga gaggcaatac tatatttctt ccacaagaac
     3061 tttgaggaag agataagaag aatgtttgag ccagggcagg agacagctgt tcctcactct
     3121 tatttcatcc acttccgttc actaggcttg agtgggaaat ctccttattc atcaaatgct
     3181 gttggtcacg tgttcaatct cattcacttt gtaggatgct atatgggtca agtcagatcc
     3241 ctaaatgcaa cggttattgc tgcatgtgct cctcatgaaa tgtctgttct agggggctat
     3301 ctgggagagg aattcttcgg gaaagggaca tttgaaagaa gattcttcag agatgagaaa
     3361 gaacttcaag aatacgaggc ggctgaactg acaaagactg acgtagcact ggcagatgat
     3421 ggaactgtca actctgacga cgaggactac ttttcaggtg aaaccagaag tccggaggct
     3481 gtttatactc gaatcatgat gaatggaggt cgactaaaga gatctcacat acggagatat
     3541 gtctcagtca gttccaatca tcaagcccgt ccaaactcat tcgccgagtt tctaaacaag
     3601 acatattcga gtgactcata agaagttgaa taacaaaatg ccggaaatct acggattgtg
     3661 tatatccatc atgaaaaaaa ctaacacccc tcctttcgaa ccatcccaaa catgagcaag
     3721 atctttgtca atcctagtgc tattagagcc ggtctggccg atcttgagat ggctgaagaa
     3781 actgttgatc tgatcaatag aaatatcgaa gacaatcagg ctcatctcca aggggaaccc
     3841 atagaggtgg acaatctccc tgaggatatg gggcgacttc acctggatga tggaaaatcg
     3901 cccaaccatg gtgagatagc caaggtggga gaaggcaagt atcgagagga ctttcagatg
     3961 gatgaaggag aggatcctag cttcctgttc cagtcatacc tggaaaatgt tggagtccaa
     4021 atagtcagac aaatgaggtc aggagagaga tttctcaaga tatggtcaca gaccgtagaa
     4081 gagattatat cctatgtcgc ggtcaacttt cccaaccctc caggaaagtc ttcagaggat
     4141 aaatcaaccc agactactgg ccgagagctc aagaaggaga caacacccac tccttctcag
     4201 agagaaagcc aatcatcgaa agccaggatg gcggctcaaa ttgcttctgg ccctccagcc
     4261 cttgaatggt cggctaccaa tgaagaggat gatctatcag tggaggctga gatcgctcac
     4321 cagattgcag aaagtttctc caaaaaatat aagtttccct ctcgatcctc agggatactc
     4381 ttgtataatt ttgagcaatt gaaaatgaac cttgatgata tagttaaaga ggcaaaaaat
     4441 gtaccaggtg tgacccgttt agcccatgac gggtccaaac tccccctaag atgtgtactg
     4501 ggatgggtcg ctttggccaa ctctaagaaa ttccagttgt tagtcgaatc cgacaagctg
     4561 agtaaaatca tgcaagatga cttgaatcgc tatacatctt gctaaccgaa cctctcccct
     4621 cagtccctct agacaataaa atccgagatg tcccaaagtc aacatgaaaa aaacaggcaa
     4681 caccactgat aaaatgaacc tcctacgtaa gatagtgaaa aaccgcaggg acgaggacac
     4741 tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat gacttgtggc ttccaccccc
     4801 tgaatacgtc ccgctgaaag aacttacagg caagaagaac atgaggaact tttgtatcaa
     4861 cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc aggatcctgc ggcacattct
     4921 gaaatcattc gacgagatat attctgggaa tcataggatg atcgggttag tcaaagtggt
     4981 tattggactg gctttgtcag gatctccagt ccctgagggc ctgaactggg tatacaaatt
     5041 gaggagaacc tttatcttcc agtgggctga ttccaggggc cctcttgaag gggaggagtt
     5101 ggaatactct caggagatca cttgggatga tgatactgag ttcgtcggat tgcaaataag
     5161 agtgattgca aaacagtgtc atatccaggg cagagtctgg tgtatcaaca tgaacccgag
     5221 agcatgtcaa ctatggtctg acatgtctct tcagacacaa aggtccgaag aggacaaaga
     5281 ttcctctctg cttctagaat aatcagatta tatcccgcaa atttatcact tgtttacctc
     5341 tggaggagag aacatatggg ctcaactcca acccttggga gcaatataac aaagaacatg
     5401 ttatggtgcc attaaaccgc tgcatttcat caaagtcaag ttgattacct ttacattttg
     5461 atcctcttgg atgtgaaaaa aactattaac atccctcaaa agaccccggg aaagccgcca
     5521 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5581 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5641 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5701 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5761 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5821 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5881 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5941 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     6001 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     6061 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6121 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6181 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     6241 aagctagctt ataaagtgct gggtcatcta agcttttcag tcgagaaaaa aacattagat
     6301 cagaagaaca actggcaaca cttctcaacc tgagacttac ttcaagatgc tcgatcctgg
     6361 agaggtctat gatgacccta ttgacccaat cgagttagag gctgaaccca gaggaacccc
     6421 cattgtcccc aacatcttga ggaactctga ctacaatctc aactctcctt tgatagaaga
     6481 tcctgctaga ctaatgttag aatggttaaa aacagggaat agaccttatc ggatgactct
     6541 aacagacaat tgctccaggt ctttcagagt tttgaaagat tatttcaaga aggtagattt
     6601 gggttctctc aaggtgggcg gaatggctgc acagtcaatg atttctctct ggttatatgg
     6661 tgcccactct gaatccaaca ggagccggag atgtataaca gacttggccc atttctattc
     6721 caagtcgtcc cccatagaga agctgttgaa tctcacgcta ggaaatagag ggctgagaat
     6781 ccccccagag ggagtgttaa gttgccttga gagggttgat tatgataatg catttggaag
     6841 gtatcttgcc aacacgtatt cctcttactt gttcttccat gtaatcacct tatacatgaa
     6901 cgccctagac tgggatgaag aaaagaccat cctagcatta tggaaagatt taacctcagt
     6961 ggacatcggg aaggacttgg taaagttcaa agaccaaata tggggactgc tgatcgtgac
     7021 aaaggacttt gtttactccc aaagttccaa ttgtcttttt gacagaaact acacacttat
     7081 gctaaaagat cttttcttgt ctcgcttcaa ctccttaatg gtcttgctct ctcccccaga
     7141 gccccgatac tcagatgact tgatatctca actatgccag ctgtacattg ctggggatca
     7201 agtcttgtct atgtgtggaa actccggcta tgaagtcatc aaaatattgg agccatatgt
     7261 cgtgaatagt ttagtccaga gagcagaaaa gtttaggcct ctcattcatt ccttgggaga
     7321 ctttcctgta tttataaaag acaaggtaag tcaacttgaa gagacgttcg gtccctgtgc
     7381 aagaaggttc tttagggctc tggatcaatt cgacaacata catgacttgg tttttgtgtt
     7441 tggctgttac aggcattggg ggcacccata tatagattat cgaaagggtc tgtcaaaact
     7501 atatgatcag gttcacctta aaaaaatgat agataagtcc taccaggagt gcttagcaag
     7561 cgacctagcc aggaggatcc ttagatgggg ttttgataag tactccaagt ggtatctgga
     7621 ttcaagattc ctagcccgag accacccctt gactccttat atcaaaaccc aaacatggcc
     7681 acccaaacat attgtagact tggtggggga tacatggcac aagctcccga tcacgcagat
     7741 ctttgagatt cctgaatcaa tggatccgtc agaaatattg gatgacaaat cacattcttt
     7801 caccagaacg agactagctt cttggctgtc agaaaaccga ggggggcctg ttcctagcga
     7861 aaaagttatt atcacggccc tgtctaagcc gcctgtcaat ccccgagagt ttctgaggtc
     7921 tatagacctc ggaggattgc cagatgaaga cttgataatt ggcctcaagc caaaggaacg
     7981 ggaattgaag attgaaggtc gattctttgc tctaatgtca tggaatctaa gattgtattt
     8041 tgtcatcact gaaaaactct tggccaacta catcttgcca ctttttgacg cgctgactat
     8101 gacagacaac ctgaacaagg tgtttaaaaa gctgatcgac agggtcaccg ggcaagggct
     8161 tttggactat tcaagggtca catatgcatt tcacctggac tatgaaaagt ggaacaacca
     8221 tcaaagatta gagtcaacag aggatgtatt ttctgtccta gatcaagtgt ttggattgaa
     8281 gagagtgttt tctagaacac acgagttttt tcaaaaggcc tggatctatt attcagacag
     8341 atcagacctc atcgggttac gggaggatca aatatactgc ttagatgcgt ccaacggccc
     8401 aacctgttgg aatggccagg atggcgggct agaaggctta cggcagaagg gctggagtct
     8461 agtcagctta ttgatgatag atagagaatc tcaaatcagg aacacaagaa ccaaaatact
     8521 agctcaagga gacaaccagg ttttatgtcc gacatacatg ttgtcgccag ggctatctca
     8581 agaggggctc ctctatgaat tggagagaat atcaaggaat gcactttcga tatacagagc
     8641 cgtcgaggaa ggggcatcta agctagggct gatcatcaag aaagaagaga ccatgtgtag
     8701 ttatgacttc ctcatctatg gaaaaacccc tttgtttaga ggtaacatat tggtgcctga
     8761 gtccaaaaga tgggccagag tctcttgcgt ctctaatgac caaatagtca acctcgccaa
     8821 tataatgtcg acagtgtcca ccaatgcgct aacagtggca caacactctc aatctttgat
     8881 caaaccgatg agggattttc tgctcatgtc agtacaggca gtctttcact acctgctatt
     8941 tagcccaatc ttaaagggaa gagtttacaa gattctgagc gctgaagggg agagctttct
     9001 cctagccatg tcaaggataa tctatctaga tccttctttg ggagggatat ctggaatgtc
     9061 cctcggaaga ttccatatac gacagttctc agaccctgtc tctgaagggt tatccttctg
     9121 gagagagatc tggttaagct cccaagagtc ctggattcac gcgttgtgtc aagaggctgg
     9181 aaacccagat cttggagaga gaacactcga gagcttcact cgccttctag aagatccgac
     9241 caccttaaat atcagaggag gggccagtcc taccattcta ctcaaggatg caatcagaaa
     9301 ggctttatat gacgaggtgg acaaggtgga aaattcagag tttcgagagg caatcctgtt
     9361 gtccaagacc catagagata attttatact cttcttaata tctgttgagc ctctgtttcc
     9421 tcgatttctc agtgagctat tcagttcgtc ttttttggga atccccgagt caatcattgg
     9481 attgatacaa aactcccgaa cgataagaag gcagtttaga aagagtctct caaaaacttt
     9541 agaagaatcc ttctacaact cagagatcca cgggattagt cggatgaccc agacacctca
     9601 gagggttggg ggggtgtggc cttgctcttc agagagggca gatctactta gggagatctc
     9661 ttggggaaga aaagtggtag gcacgacagt tcctcaccct tctgagatgt tgggattact
     9721 tcccaagtcc tctatttctt gcacttgtgg agcaacagga ggaggcaatc ctagagtttc
     9781 tgtatcagta ctcccgtcct ttgatcagtc atttttttca cgaggccccc taaagggata
     9841 cttgggctcg tccacctcta tgtcgaccca gctattccat gcatgggaaa aagtcactaa
     9901 tgttcatgtg gtgaagagag ctctatcgtt aaaagaatct ataaactggt tcattactag
     9961 agattccaac ttggctcaag ctctaattag gaacattatg tctctgacag gccctgattt
    10021 ccctctagag gaggcccctg tcttcaaaag gacggggtca gccttgcata ggttcaagtc
    10081 tgccagatac agcgaaggag ggtattcttc tgtctgcccg aacctcctct ctcatatttc
    10141 tgttagtaca gacaccatgt ctgatttgac ccaagacggg aagaactacg atttcatgtt
    10201 ccagccattg atgctttatg cacagacatg gacatcagag ctggtacaga gagacacaag
    10261 gctaagagac tctacgtttc attggcacct ccgatgcaac aggtgtgtga gacccattga
    10321 cgacgtgacc ctggagacct ctcagatctt cgagtttccg gatgtgtcga aaagaatatc
    10381 cagaatggtt tctggggctg tgcctcactt ccagaggctt cccgatatcc gtctgagacc
    10441 aggagatttt gaatctctaa gcggtagaga aaagtctcac catatcggat cagctcaggg
    10501 gctcttatac tcaatcttag tggcaattca cgactcagga tacaatgatg gaaccatctt
    10561 ccctgtcaac atatacggca aggtttcccc tagagactat ttgagagggc tcgcaagggg
    10621 agtattgata ggatcctcga tttgcttctt gacaagaatg acaaatatca atattaatag
    10681 acctcttgaa ttggtctcag gggtaatctc atatattctc ctgaggctag ataaccatcc
    10741 ctccttgtac ataatgctca gagaaccgtc tcttagagga gagatatttt ctatccctca
    10801 gaaaatcccc gccgcttatc caaccactat gaaagaaggc aacagatcaa tcttgtgtta
    10861 tctccaacat gtgctacgct atgagcgaga gataatcacg gcgtctccag agaatgactg
    10921 gctatggatc ttttcagact ttagaagtgc caaaatgacg tacctatccc tcattactta
    10981 ccagtctcat cttctactcc agagggttga gagaaaccta tctaagagta tgagagataa
    11041 cctgcgacaa ttgagttctt tgatgaggca ggtgctgggc gggcacggag aagatacctt
    11101 agagtcagac gacaacattc aacgactgct aaaagactct ttacgaagga caagatgggt
    11161 ggatcaagag gtgcgccatg cagctagaac catgactgga gattacagcc ccaacaagaa
    11221 ggtgtcccgt aaggtaggat gttcagaatg ggtctgctct gctcaacagg ttgcagtctc
    11281 tacctcagca aacccggccc ctgtctcgga gcttgacata agggccctct ctaagaggtt
    11341 ccagaaccct ttgatctcgg gcttgagagt ggttcagtgg gcaaccggtg ctcattataa
    11401 gcttaagcct attctagatg atctcaatgt tttcccatct ctctgccttg tagttgggga
    11461 cgggtcaggg gggatatcaa gggcagtcct caacatgttt ccagatgcca agcttgtgtt
    11521 caacagtctt ttagaggtga atgacctgat ggcttccgga acacatccac tgcctccttc
    11581 agcaatcatg aggggaggaa atgatatcgt ctccagagtg atagatcttg actcaatctg
    11641 ggaaaaaccg tccgacttga gaaacttggc aacctggaaa tacttccagt cagtccaaaa
    11701 gcaggtcaac atgtcctatg acctcattat ttgcgatgca gaagttactg acattgcatc
    11761 tatcaaccgg atcaccctgt taatgtccga ttttgcattg tctatagatg gaccactcta
    11821 tttggtcttc aaaacttatg ggactatgct agtaaatcca aactacaagg ctattcaaca
    11881 cctgtcaaga gcgttcccct cggtcacagg gtttatcacc caagtaactt cgtctttttc
    11941 atctgagctc tacctccgat tctccaaacg agggaagttt ttcagagatg ctgagtactt
    12001 gacctcttcc acccttcgag aaatgagcct tgtgttattc aattgtagca gccccaagag
    12061 tgagatgcag agagctcgtt ccttgaacta tcaggatctt gtgagaggat ttcctgaaga
    12121 aatcatatca aatccttaca atgagatgat cataactctg attgacagtg atgtagaatc
    12181 ttttctagtc cacaagatgg ttgatgatct tgagttacag aggggaactc tgtctaaagt
    12241 ggctatcatt atagccatca tgatagtttt ctccaacaga gtcttcaacg tttccaaacc
    12301 cctaactgac ccctcgttct atccaccgtc tgatcccaaa atcctgaggc acttcaacat
    12361 atgttgcagt actatgatgt atctatctac tgctttaggt gacgtcccta gcttcgcaag
    12421 acttcacgac ctgtataaca gacctataac ttattacttc agaaagcaag tcattcgagg
    12481 gaacgtttat ctatcttgga gttggtccaa cgacacctca gtgttcaaaa gggtagcctg
    12541 taattctagc ctgagtctgt catctcactg gatcaggttg atttacaaga tagtgaagac
    12601 taccagactc gttggcagca tcaaggatct atccagagaa gtggaaagac accttcatag
    12661 gtacaacagg tggatcaccc tagaggatat cagatctaga tcatccctac tagactacag
    12721 ttgcctgtga accggatact cctggaagcc tgcccatgct aagactcttg tgtgatgtat
    12781 cttgaaaaaa acaagatcct aaatctgaac ctttggttgt ttgattgttt ttctcatttt
    12841 tgttgtttat ttgttaagcg tgggtcggca tggcatctcc acctcctcgc ggtccgacct
    12901 gggcatccga aggaggacgc acgtccactc ggatggctaa gggaggggcg gggatccggc
    12961 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
    13021 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
    13081 atccggatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
    13141 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    13201 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    13261 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    13321 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    13381 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    13441 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    13501 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    13561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    13621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    13681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    13741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    13801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    13861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    13921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    13981 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    14041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    14101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    14161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    14221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    14281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    14341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    14401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    14461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    14521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    14581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    14641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    14701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    14761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    14821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    14881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    14941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    15001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    15061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    15121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    15181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    15241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    15301 cgcgcacatt tccccgaaaa gtgccacctg
//
