LOCUS       sequence-81370-e       17093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry, EGFP and Synaptophysin-TagRFP-T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 17093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-mCherry-N-P-M-EGFP-SynPhRFP-L
FEATURES             Location/Qualifiers
     source          1..17093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1453..1462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1459..2169
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1604..1622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1872..1891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      5517..5526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5523..5525,5526..5528,5529..6242)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5523..5525,5526..5528,5529..6239)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5568..5589)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5829..5848)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6176..6197
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      6306..6315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7266..7275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7272..8003
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             9755..9766
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(14765..14783)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      14779..14826
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(14849..14865)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14849..14865)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(14902..14922)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14902..14920)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14941..14957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14941..14957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14954..14976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14965..14981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14989..14995,14996..15013,15014..15019))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15034..15055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15172..15189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15343..15931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15423..15442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16102..16893,16894..16962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16725..16744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16963..17067)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 ccggtgaaag ccgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     1501 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1561 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1621 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1681 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1741 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1801 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1861 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1921 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1981 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2041 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2101 atcgtggaac agtacgaacg cgccgagggc cgccactcta ccggcggcat ggacgagctg
     2161 tacaagtaat gacctaggga agttgaataa caaaatgccg gaaatctacg gattgtgtat
     2221 atccatcatg aaaaaaacta acacccctcc tttcgaacca tcccaaacat ggatgccgac
     2281 aagattgtat tcaaagtcaa taatcaggtg gtctctttga agcctgagat tatcgtggat
     2341 caatatgagt acaagtaccc tgccatcaaa gatttgaaaa agccctgtat aactctagga
     2401 aaggctcccg atttaaataa agcatacaag tcagttttgt caggcatgag cgccgccaaa
     2461 cttaatcctg acgatgtatg ttcctatttg gcagcggcaa tgcagttttt tgaggggaca
     2521 tgtccggaag actggaccag ctatggaatt gtgattgcac gaaaaggaga taagatcacc
     2581 ccaggttctc tggtggagat aaaacgtact gatgtagaag ggaattgggc tctgacagga
     2641 ggcatggaac tgacaagaga ccccactgtc cctgagcatg cgtccttagt cggtcttctc
     2701 ttgagtctgt ataggttgag caaaatatcc gggcaaaaca ctggtaacta taagacaaac
     2761 attgcagaca ggatagagca gatttttgag acagcccctt ttgttaaaat cgtggaacac
     2821 catactctaa tgacaactca caaaatgtgt gctaattgga gtactatacc aaacttcaga
     2881 tttttggccg gaacctatga catgtttttc tcccggattg agcatctata ttcagcaatc
     2941 agagtgggca cagttgtcac tgcttatgaa gactgttcag gactggtatc atttactggg
     3001 ttcataaaac aaatcaatct caccgctaga gaggcaatac tatatttctt ccacaagaac
     3061 tttgaggaag agataagaag aatgtttgag ccagggcagg agacagctgt tcctcactct
     3121 tatttcatcc acttccgttc actaggcttg agtgggaaat ctccttattc atcaaatgct
     3181 gttggtcacg tgttcaatct cattcacttt gtaggatgct atatgggtca agtcagatcc
     3241 ctaaatgcaa cggttattgc tgcatgtgct cctcatgaaa tgtctgttct agggggctat
     3301 ctgggagagg aattcttcgg gaaagggaca tttgaaagaa gattcttcag agatgagaaa
     3361 gaacttcaag aatacgaggc ggctgaactg acaaagactg acgtagcact ggcagatgat
     3421 ggaactgtca actctgacga cgaggactac ttttcaggtg aaaccagaag tccggaggct
     3481 gtttatactc gaatcatgat gaatggaggt cgactaaaga gatctcacat acggagatat
     3541 gtctcagtca gttccaatca tcaagcccgt ccaaactcat tcgccgagtt tctaaacaag
     3601 acatattcga gtgactcata agaagttgaa taacaaaatg ccggaaatct acggattgtg
     3661 tatatccatc atgaaaaaaa ctaacacccc tcctttcgaa ccatcccaaa catgagcaag
     3721 atctttgtca atcctagtgc tattagagcc ggtctggccg atcttgagat ggctgaagaa
     3781 actgttgatc tgatcaatag aaatatcgaa gacaatcagg ctcatctcca aggggaaccc
     3841 atagaggtgg acaatctccc tgaggatatg gggcgacttc acctggatga tggaaaatcg
     3901 cccaaccatg gtgagatagc caaggtggga gaaggcaagt atcgagagga ctttcagatg
     3961 gatgaaggag aggatcctag cttcctgttc cagtcatacc tggaaaatgt tggagtccaa
     4021 atagtcagac aaatgaggtc aggagagaga tttctcaaga tatggtcaca gaccgtagaa
     4081 gagattatat cctatgtcgc ggtcaacttt cccaaccctc caggaaagtc ttcagaggat
     4141 aaatcaaccc agactactgg ccgagagctc aagaaggaga caacacccac tccttctcag
     4201 agagaaagcc aatcatcgaa agccaggatg gcggctcaaa ttgcttctgg ccctccagcc
     4261 cttgaatggt cggctaccaa tgaagaggat gatctatcag tggaggctga gatcgctcac
     4321 cagattgcag aaagtttctc caaaaaatat aagtttccct ctcgatcctc agggatactc
     4381 ttgtataatt ttgagcaatt gaaaatgaac cttgatgata tagttaaaga ggcaaaaaat
     4441 gtaccaggtg tgacccgttt agcccatgac gggtccaaac tccccctaag atgtgtactg
     4501 ggatgggtcg ctttggccaa ctctaagaaa ttccagttgt tagtcgaatc cgacaagctg
     4561 agtaaaatca tgcaagatga cttgaatcgc tatacatctt gctaaccgaa cctctcccct
     4621 cagtccctct agacaataaa atccgagatg tcccaaagtc aacatgaaaa aaacaggcaa
     4681 caccactgat aaaatgaacc tcctacgtaa gatagtgaaa aaccgcaggg acgaggacac
     4741 tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat gacttgtggc ttccaccccc
     4801 tgaatacgtc ccgctgaaag aacttacagg caagaagaac atgaggaact tttgtatcaa
     4861 cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc aggatcctgc ggcacattct
     4921 gaaatcattc gacgagatat attctgggaa tcataggatg atcgggttag tcaaagtggt
     4981 tattggactg gctttgtcag gatctccagt ccctgagggc ctgaactggg tatacaaatt
     5041 gaggagaacc tttatcttcc agtgggctga ttccaggggc cctcttgaag gggaggagtt
     5101 ggaatactct caggagatca cttgggatga tgatactgag ttcgtcggat tgcaaataag
     5161 agtgattgca aaacagtgtc atatccaggg cagagtctgg tgtatcaaca tgaacccgag
     5221 agcatgtcaa ctatggtctg acatgtctct tcagacacaa aggtccgaag aggacaaaga
     5281 ttcctctctg cttctagaat aatcagatta tatcccgcaa atttatcact tgtttacctc
     5341 tggaggagag aacatatggg ctcaactcca acccttggga gcaatataac aaagaacatg
     5401 ttatggtgcc attaaaccgc tgcatttcat caaagtcaag ttgattacct ttacattttg
     5461 atcctcttgg atgtgaaaaa aactattaac atccctcaaa agaccccggg aaagccgcca
     5521 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5581 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5641 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5701 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5761 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5821 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5881 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5941 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     6001 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     6061 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6121 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6181 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     6241 aattaattaa cgtcctttca acgatccaag tccatgaaaa aaactaacac ccctcccgta
     6301 cggccgccac catggacgtg gtgaatcagc tggtggctgg gggtcagttc cgggtggtca
     6361 aggagcccct tggcttcgtg aaggtgctgc agtgggtctt tgccatcttc gcctttgcta
     6421 cgtgcggcag ctacaccgga gagcttcggc tgagcgtgga gtgtgccaac aagacggaga
     6481 gtgccctcaa catcgaagtc gaatttgagt acccattcag gctgcaccaa gtgtactttg
     6541 atgcaccctc ctgcgttaaa gggggcacta ccaagatctt cctagttggt gactactcct
     6601 cctcgggtga attctttgtc accgtggctg tgtttgcctt cctctactcc atgggggccc
     6661 tggccaccta catcttcctg cagaacaagt accgagagaa caacaaaggg ccaatgatgg
     6721 acttcctggc cacagcagtg ttcgctttca tgtggctagt tagctcatcc gcctgggcca
     6781 aaggcctgtc cgatgtgaag atggccactg acccagagaa cattatcaag gagatgccta
     6841 tgtgccgcca gacaggaaac acatgcaagg aactgaggga ccctgtgact tcaggactca
     6901 acacctcggt ggtgtttggc ttcctgaacc tggtgctctg ggttggcaac ctatggttcg
     6961 tgttcaagga gacaggctgg gccgccccat tcatgcgcgc acctccaggc gccccagaaa
     7021 agcaaccagc tcctggcgat gcctacggcg atgcgggcta tgggcagggc cccggaggct
     7081 atgggcccca ggactcctac gggcctcagg gtggttatca acccgattac gggcagccag
     7141 ccagcggcgg tggcggtggc tacgggcctc agggcgacta tgggcagcaa ggctacggcc
     7201 aacagggtgc gcccacctcc ttctccaatc agatgggcgg aggccctggg atccaccggc
     7261 cggtcgccac catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc
     7321 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca
     7381 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
     7441 tcgccttcga catcctggct accagcttca tgtacggcag cagaaccttc atcaaccaca
     7501 cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag
     7561 tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
     7621 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga
     7681 tgcagaagaa aacactcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg
     7741 gcctggaagg cagaaccgac atggccctga agctcgtggg cgggggccac ctgatctgca
     7801 acttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct
     7861 actatgtgga ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc
     7921 agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta
     7981 atggcatgga cgagctgtac aagtaagcta gcttataaag tgctgggtca tctaagcttt
     8041 tcagtcgaga aaaaaacatt agatcagaag aacaactggc aacacttctc aacctgagac
     8101 ttacttcaag atgctcgatc ctggagaggt ctatgatgac cctattgacc caatcgagtt
     8161 agaggctgaa cccagaggaa cccccattgt ccccaacatc ttgaggaact ctgactacaa
     8221 tctcaactct cctttgatag aagatcctgc tagactaatg ttagaatggt taaaaacagg
     8281 gaatagacct tatcggatga ctctaacaga caattgctcc aggtctttca gagttttgaa
     8341 agattatttc aagaaggtag atttgggttc tctcaaggtg ggcggaatgg ctgcacagtc
     8401 aatgatttct ctctggttat atggtgccca ctctgaatcc aacaggagcc ggagatgtat
     8461 aacagacttg gcccatttct attccaagtc gtcccccata gagaagctgt tgaatctcac
     8521 gctaggaaat agagggctga gaatcccccc agagggagtg ttaagttgcc ttgagagggt
     8581 tgattatgat aatgcatttg gaaggtatct tgccaacacg tattcctctt acttgttctt
     8641 ccatgtaatc accttataca tgaacgccct agactgggat gaagaaaaga ccatcctagc
     8701 attatggaaa gatttaacct cagtggacat cgggaaggac ttggtaaagt tcaaagacca
     8761 aatatgggga ctgctgatcg tgacaaagga ctttgtttac tcccaaagtt ccaattgtct
     8821 ttttgacaga aactacacac ttatgctaaa agatcttttc ttgtctcgct tcaactcctt
     8881 aatggtcttg ctctctcccc cagagccccg atactcagat gacttgatat ctcaactatg
     8941 ccagctgtac attgctgggg atcaagtctt gtctatgtgt ggaaactccg gctatgaagt
     9001 catcaaaata ttggagccat atgtcgtgaa tagtttagtc cagagagcag aaaagtttag
     9061 gcctctcatt cattccttgg gagactttcc tgtatttata aaagacaagg taagtcaact
     9121 tgaagagacg ttcggtccct gtgcaagaag gttctttagg gctctggatc aattcgacaa
     9181 catacatgac ttggtttttg tgtttggctg ttacaggcat tgggggcacc catatataga
     9241 ttatcgaaag ggtctgtcaa aactatatga tcaggttcac cttaaaaaaa tgatagataa
     9301 gtcctaccag gagtgcttag caagcgacct agccaggagg atccttagat ggggttttga
     9361 taagtactcc aagtggtatc tggattcaag attcctagcc cgagaccacc ccttgactcc
     9421 ttatatcaaa acccaaacat ggccacccaa acatattgta gacttggtgg gggatacatg
     9481 gcacaagctc ccgatcacgc agatctttga gattcctgaa tcaatggatc cgtcagaaat
     9541 attggatgac aaatcacatt ctttcaccag aacgagacta gcttcttggc tgtcagaaaa
     9601 ccgagggggg cctgttccta gcgaaaaagt tattatcacg gccctgtcta agccgcctgt
     9661 caatccccga gagtttctga ggtctataga cctcggagga ttgccagatg aagacttgat
     9721 aattggcctc aagccaaagg aacgggaatt gaagattgaa ggtcgattct ttgctctaat
     9781 gtcatggaat ctaagattgt attttgtcat cactgaaaaa ctcttggcca actacatctt
     9841 gccacttttt gacgcgctga ctatgacaga caacctgaac aaggtgttta aaaagctgat
     9901 cgacagggtc accgggcaag ggcttttgga ctattcaagg gtcacatatg catttcacct
     9961 ggactatgaa aagtggaaca accatcaaag attagagtca acagaggatg tattttctgt
    10021 cctagatcaa gtgtttggat tgaagagagt gttttctaga acacacgagt tttttcaaaa
    10081 ggcctggatc tattattcag acagatcaga cctcatcggg ttacgggagg atcaaatata
    10141 ctgcttagat gcgtccaacg gcccaacctg ttggaatggc caggatggcg ggctagaagg
    10201 cttacggcag aagggctgga gtctagtcag cttattgatg atagatagag aatctcaaat
    10261 caggaacaca agaaccaaaa tactagctca aggagacaac caggttttat gtccgacata
    10321 catgttgtcg ccagggctat ctcaagaggg gctcctctat gaattggaga gaatatcaag
    10381 gaatgcactt tcgatataca gagccgtcga ggaaggggca tctaagctag ggctgatcat
    10441 caagaaagaa gagaccatgt gtagttatga cttcctcatc tatggaaaaa cccctttgtt
    10501 tagaggtaac atattggtgc ctgagtccaa aagatgggcc agagtctctt gcgtctctaa
    10561 tgaccaaata gtcaacctcg ccaatataat gtcgacagtg tccaccaatg cgctaacagt
    10621 ggcacaacac tctcaatctt tgatcaaacc gatgagggat tttctgctca tgtcagtaca
    10681 ggcagtcttt cactacctgc tatttagccc aatcttaaag ggaagagttt acaagattct
    10741 gagcgctgaa ggggagagct ttctcctagc catgtcaagg ataatctatc tagatccttc
    10801 tttgggaggg atatctggaa tgtccctcgg aagattccat atacgacagt tctcagaccc
    10861 tgtctctgaa gggttatcct tctggagaga gatctggtta agctcccaag agtcctggat
    10921 tcacgcgttg tgtcaagagg ctggaaaccc agatcttgga gagagaacac tcgagagctt
    10981 cactcgcctt ctagaagatc cgaccacctt aaatatcaga ggaggggcca gtcctaccat
    11041 tctactcaag gatgcaatca gaaaggcttt atatgacgag gtggacaagg tggaaaattc
    11101 agagtttcga gaggcaatcc tgttgtccaa gacccataga gataatttta tactcttctt
    11161 aatatctgtt gagcctctgt ttcctcgatt tctcagtgag ctattcagtt cgtctttttt
    11221 gggaatcccc gagtcaatca ttggattgat acaaaactcc cgaacgataa gaaggcagtt
    11281 tagaaagagt ctctcaaaaa ctttagaaga atccttctac aactcagaga tccacgggat
    11341 tagtcggatg acccagacac ctcagagggt tgggggggtg tggccttgct cttcagagag
    11401 ggcagatcta cttagggaga tctcttgggg aagaaaagtg gtaggcacga cagttcctca
    11461 cccttctgag atgttgggat tacttcccaa gtcctctatt tcttgcactt gtggagcaac
    11521 aggaggaggc aatcctagag tttctgtatc agtactcccg tcctttgatc agtcattttt
    11581 ttcacgaggc cccctaaagg gatacttggg ctcgtccacc tctatgtcga cccagctatt
    11641 ccatgcatgg gaaaaagtca ctaatgttca tgtggtgaag agagctctat cgttaaaaga
    11701 atctataaac tggttcatta ctagagattc caacttggct caagctctaa ttaggaacat
    11761 tatgtctctg acaggccctg atttccctct agaggaggcc cctgtcttca aaaggacggg
    11821 gtcagccttg cataggttca agtctgccag atacagcgaa ggagggtatt cttctgtctg
    11881 cccgaacctc ctctctcata tttctgttag tacagacacc atgtctgatt tgacccaaga
    11941 cgggaagaac tacgatttca tgttccagcc attgatgctt tatgcacaga catggacatc
    12001 agagctggta cagagagaca caaggctaag agactctacg tttcattggc acctccgatg
    12061 caacaggtgt gtgagaccca ttgacgacgt gaccctggag acctctcaga tcttcgagtt
    12121 tccggatgtg tcgaaaagaa tatccagaat ggtttctggg gctgtgcctc acttccagag
    12181 gcttcccgat atccgtctga gaccaggaga ttttgaatct ctaagcggta gagaaaagtc
    12241 tcaccatatc ggatcagctc aggggctctt atactcaatc ttagtggcaa ttcacgactc
    12301 aggatacaat gatggaacca tcttccctgt caacatatac ggcaaggttt cccctagaga
    12361 ctatttgaga gggctcgcaa ggggagtatt gataggatcc tcgatttgct tcttgacaag
    12421 aatgacaaat atcaatatta atagacctct tgaattggtc tcaggggtaa tctcatatat
    12481 tctcctgagg ctagataacc atccctcctt gtacataatg ctcagagaac cgtctcttag
    12541 aggagagata ttttctatcc ctcagaaaat ccccgccgct tatccaacca ctatgaaaga
    12601 aggcaacaga tcaatcttgt gttatctcca acatgtgcta cgctatgagc gagagataat
    12661 cacggcgtct ccagagaatg actggctatg gatcttttca gactttagaa gtgccaaaat
    12721 gacgtaccta tccctcatta cttaccagtc tcatcttcta ctccagaggg ttgagagaaa
    12781 cctatctaag agtatgagag ataacctgcg acaattgagt tctttgatga ggcaggtgct
    12841 gggcgggcac ggagaagata ccttagagtc agacgacaac attcaacgac tgctaaaaga
    12901 ctctttacga aggacaagat gggtggatca agaggtgcgc catgcagcta gaaccatgac
    12961 tggagattac agccccaaca agaaggtgtc ccgtaaggta ggatgttcag aatgggtctg
    13021 ctctgctcaa caggttgcag tctctacctc agcaaacccg gcccctgtct cggagcttga
    13081 cataagggcc ctctctaaga ggttccagaa ccctttgatc tcgggcttga gagtggttca
    13141 gtgggcaacc ggtgctcatt ataagcttaa gcctattcta gatgatctca atgttttccc
    13201 atctctctgc cttgtagttg gggacgggtc aggggggata tcaagggcag tcctcaacat
    13261 gtttccagat gccaagcttg tgttcaacag tcttttagag gtgaatgacc tgatggcttc
    13321 cggaacacat ccactgcctc cttcagcaat catgagggga ggaaatgata tcgtctccag
    13381 agtgatagat cttgactcaa tctgggaaaa accgtccgac ttgagaaact tggcaacctg
    13441 gaaatacttc cagtcagtcc aaaagcaggt caacatgtcc tatgacctca ttatttgcga
    13501 tgcagaagtt actgacattg catctatcaa ccggatcacc ctgttaatgt ccgattttgc
    13561 attgtctata gatggaccac tctatttggt cttcaaaact tatgggacta tgctagtaaa
    13621 tccaaactac aaggctattc aacacctgtc aagagcgttc ccctcggtca cagggtttat
    13681 cacccaagta acttcgtctt tttcatctga gctctacctc cgattctcca aacgagggaa
    13741 gtttttcaga gatgctgagt acttgacctc ttccaccctt cgagaaatga gccttgtgtt
    13801 attcaattgt agcagcccca agagtgagat gcagagagct cgttccttga actatcagga
    13861 tcttgtgaga ggatttcctg aagaaatcat atcaaatcct tacaatgaga tgatcataac
    13921 tctgattgac agtgatgtag aatcttttct agtccacaag atggttgatg atcttgagtt
    13981 acagagggga actctgtcta aagtggctat cattatagcc atcatgatag ttttctccaa
    14041 cagagtcttc aacgtttcca aacccctaac tgacccctcg ttctatccac cgtctgatcc
    14101 caaaatcctg aggcacttca acatatgttg cagtactatg atgtatctat ctactgcttt
    14161 aggtgacgtc cctagcttcg caagacttca cgacctgtat aacagaccta taacttatta
    14221 cttcagaaag caagtcattc gagggaacgt ttatctatct tggagttggt ccaacgacac
    14281 ctcagtgttc aaaagggtag cctgtaattc tagcctgagt ctgtcatctc actggatcag
    14341 gttgatttac aagatagtga agactaccag actcgttggc agcatcaagg atctatccag
    14401 agaagtggaa agacaccttc ataggtacaa caggtggatc accctagagg atatcagatc
    14461 tagatcatcc ctactagact acagttgcct gtgaaccgga tactcctgga agcctgccca
    14521 tgctaagact cttgtgtgat gtatcttgaa aaaaacaaga tcctaaatct gaacctttgg
    14581 ttgtttgatt gtttttctca tttttgttgt ttatttgtta agcgtgggtc ggcatggcat
    14641 ctccacctcc tcgcggtccg acctgggcat ccgaaggagg acgcacgtcc actcggatgg
    14701 ctaagggagg gcggggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
    14761 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
    14821 tttttgctga aaggaggaac tatatccgga tccactagtt ctagagcggc cgccaccgcg
    14881 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
    14941 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    15001 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    15061 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    15121 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    15181 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    15241 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    15301 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    15361 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    15421 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    15481 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    15541 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    15601 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    15661 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    15721 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    15781 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    15841 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    15901 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    15961 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    16021 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    16081 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    16141 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    16201 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    16261 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    16321 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    16381 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    16441 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    16501 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    16561 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    16621 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    16681 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    16741 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    16801 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    16861 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    16921 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    16981 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    17041 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
