LOCUS       sequence-81371-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLSL with Zif268:FokI coding sequence followed by approximately 3kb 
            'filler' sequence, Rep is not included on this plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLZ
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(813..833)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(867..891)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtgtcaatg taccagcttt aatcatttct ccaccaatca aaagctcttc tacactaaga
       61 acagctccat tacaattagt gatatgattc aatcttgtaa gctgagcttt gtagtttcct
      121 ttgaaatgac cactcacaaa caagaactta aattctgtta ctgaagatgg atagactttc
      181 caccattcat tagggttgat atgcttgttt cttgtttgat tctcttcgac atatctttgc
      241 atttcatctg cttgaccaat tggaagatta taacctcctg aatatgcctt agtatcaacg
      301 atcacaccgt aatcaatagg agatccgaca gtataaattg ctccgtctgg tttccttgat
      361 ccacccaaat gtttaccacg ataaccataa accttcataa agaactccat caccttcatt
      421 tcaaggattc tatcctgagt tgaatttctt gcgatttcaa tcaattcaat atattcatgt
      481 ggcacatatt tcaatttatg tctaagttca gatttcttct cttccaattc agatttcact
      541 agttccttct gacgaagatg gatctttgta tgacgcttcc tttcatcact tcttgcgaac
      601 ttacgaccac agatgtcaca tgcgaatggt ttctcaccag tatgggttct gatatgtgta
      661 gtcaaatgat cgctacgact aaagttcctc atacagattc tacactggaa aggcttctgt
      721 cctgtgtgga ttctaatatg cctagtgagt tcgtctgacc tactaaaacg tctatcacaa
      781 gactcaactg gacaagcgta aggcaattca agaacctttc tctttttctt tggaggggag
      841 gaagccatgg tgaagggcga attcggagcc tgcttttttg tacaaacttg tagaaatgat
      901 ttaaattgct gcaatagaag tagaatgctt gattgcttga gattcgtttg ttttgtatat
      961 gttgtgttga gaactctcga cgtcctgcaa acaatacaca acaaagacaa tgagatttgg
     1021 ttttataaac aatcaagaat cttagggagg gtcgtacgaa taattcgtat ccaacggaaa
     1081 tacctg
//
