LOCUS       sequence-81373-e       17553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mOrange2 and PSD-95-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17553)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 17553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-1mOrange2-5PSD95EGFP
FEATURES             Location/Qualifiers
     source          1..17553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1452..1461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1458..2168
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1871..1890
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      5514..5523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5556..5585
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(7746..7748,7749..7751,7752..8465)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7746..7748,7749..7751,7752..8462)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7791..7812)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8052..8071)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8399..8420
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10214..10225
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(15225..15243)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      15239..15286
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(15309..15325)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15309..15325)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(15362..15382)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(15362..15380)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15401..15417)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15401..15417)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15414..15436)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15425..15441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15449..15455,15456..15473,15474..15479))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15494..15515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15632..15649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15803..16391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15883..15902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16562..17353,17354..17422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17185..17204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17423..17527)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 ccgggaaagc cgccaccatg gtgagcaagg gcgaggagaa taacatggcc atcatcaagg
     1501 agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg
     1561 agggcgaggg cgagggccgc ccctacgagg gctttcagac cgctaagctg aaggtgacca
     1621 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcatttcacc tacggctcca
     1681 aggcctacgt gaagcacccc gccgacatcc ccgactactt caagctgtcc ttccccgagg
     1741 gcttcaagtg ggagcgcgtg atgaactacg aggacggcgg cgtggtgacc gtgacccagg
     1801 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     1861 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     1921 tgtaccccga ggacggcgcc ctgaagggca agatcaagat gaggctgaag ctgaaggacg
     1981 gcggccacta cacctccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     2041 ccggcgccta catcgtcgac atcaagttgg acatcacctc ccacaacgag gactacacca
     2101 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     2161 acaagtaagc tagggaagtt gaataacaaa atgccggaaa tctacggatt gtgtatatcc
     2221 atcatgaaaa aaactaacac ccctcctttc gaaccatccc aaacatggat gccgacaaga
     2281 ttgtattcaa agtcaataat caggtggtct ctttgaagcc tgagattatc gtggatcaat
     2341 atgagtacaa gtaccctgcc atcaaagatt tgaaaaagcc ctgtataact ctaggaaagg
     2401 ctcccgattt aaataaagca tacaagtcag ttttgtcagg catgagcgcc gccaaactta
     2461 atcctgacga tgtatgttcc tatttggcag cggcaatgca gttttttgag gggacatgtc
     2521 cggaagactg gaccagctat ggaattgtga ttgcacgaaa aggagataag atcaccccag
     2581 gttctctggt ggagataaaa cgtactgatg tagaagggaa ttgggctctg acaggaggca
     2641 tggaactgac aagagacccc actgtccctg agcatgcgtc cttagtcggt cttctcttga
     2701 gtctgtatag gttgagcaaa atatccgggc aaaacactgg taactataag acaaacattg
     2761 cagacaggat agagcagatt tttgagacag ccccttttgt taaaatcgtg gaacaccata
     2821 ctctaatgac aactcacaaa atgtgtgcta attggagtac tataccaaac ttcagatttt
     2881 tggccggaac ctatgacatg tttttctccc ggattgagca tctatattca gcaatcagag
     2941 tgggcacagt tgtcactgct tatgaagact gttcaggact ggtatcattt actgggttca
     3001 taaaacaaat caatctcacc gctagagagg caatactata tttcttccac aagaactttg
     3061 aggaagagat aagaagaatg tttgagccag ggcaggagac agctgttcct cactcttatt
     3121 tcatccactt ccgttcacta ggcttgagtg ggaaatctcc ttattcatca aatgctgttg
     3181 gtcacgtgtt caatctcatt cactttgtag gatgctatat gggtcaagtc agatccctaa
     3241 atgcaacggt tattgctgca tgtgctcctc atgaaatgtc tgttctaggg ggctatctgg
     3301 gagaggaatt cttcgggaaa gggacatttg aaagaagatt cttcagagat gagaaagaac
     3361 ttcaagaata cgaggcggct gaactgacaa agactgacgt agcactggca gatgatggaa
     3421 ctgtcaactc tgacgacgag gactactttt caggtgaaac cagaagtccg gaggctgttt
     3481 atactcgaat catgatgaat ggaggtcgac taaagagatc tcacatacgg agatatgtct
     3541 cagtcagttc caatcatcaa gcccgtccaa actcattcgc cgagtttcta aacaagacat
     3601 attcgagtga ctcataagaa gttgaataac aaaatgccgg aaatctacgg attgtgtata
     3661 tccatcatga aaaaaactaa cacccctcct ttcgaaccat cccaaacatg agcaagatct
     3721 ttgtcaatcc tagtgctatt agagccggtc tggccgatct tgagatggct gaagaaactg
     3781 ttgatctgat caatagaaat atcgaagaca atcaggctca tctccaaggg gaacccatag
     3841 aggtggacaa tctccctgag gatatggggc gacttcacct ggatgatgga aaatcgccca
     3901 accatggtga gatagccaag gtgggagaag gcaagtatcg agaggacttt cagatggatg
     3961 aaggagagga tcctagcttc ctgttccagt catacctgga aaatgttgga gtccaaatag
     4021 tcagacaaat gaggtcagga gagagatttc tcaagatatg gtcacagacc gtagaagaga
     4081 ttatatccta tgtcgcggtc aactttccca accctccagg aaagtcttca gaggataaat
     4141 caacccagac tactggccga gagctcaaga aggagacaac acccactcct tctcagagag
     4201 aaagccaatc atcgaaagcc aggatggcgg ctcaaattgc ttctggccct ccagcccttg
     4261 aatggtcggc taccaatgaa gaggatgatc tatcagtgga ggctgagatc gctcaccaga
     4321 ttgcagaaag tttctccaaa aaatataagt ttccctctcg atcctcaggg atactcttgt
     4381 ataattttga gcaattgaaa atgaaccttg atgatatagt taaagaggca aaaaatgtac
     4441 caggtgtgac ccgtttagcc catgacgggt ccaaactccc cctaagatgt gtactgggat
     4501 gggtcgcttt ggccaactct aagaaattcc agttgttagt cgaatccgac aagctgagta
     4561 aaatcatgca agatgacttg aatcgctata catcttgcta accgaacctc tcccctcagt
     4621 ccctctagac aataaaatcc gagatgtccc aaagtcaaca tgaaaaaaac aggcaacacc
     4681 actgataaaa tgaacctcct acgtaagata gtgaaaaacc gcagggacga ggacactcaa
     4741 aaatcctctc ccgcgtcagc ccctctggat gacgatgact tgtggcttcc accccctgaa
     4801 tacgtcccgc tgaaagaact tacaggcaag aagaacatga ggaacttttg tatcaacgga
     4861 agggttaaag tgtgtagccc gaatggttac tcgttcagga tcctgcggca cattctgaaa
     4921 tcattcgacg agatatattc tgggaatcat aggatgatcg ggttagtcaa agtggttatt
     4981 ggactggctt tgtcaggatc tccagtccct gagggcctga actgggtata caaattgagg
     5041 agaaccttta tcttccagtg ggctgattcc aggggccctc ttgaagggga ggagttggaa
     5101 tactctcagg agatcacttg ggatgatgat actgagttcg tcggattgca aataagagtg
     5161 attgcaaaac agtgtcatat ccagggcaga gtctggtgta tcaacatgaa cccgagagca
     5221 tgtcaactat ggtctgacat gtctcttcag acacaaaggt ccgaagagga caaagattcc
     5281 tctctgcttc tagaataatc agattatatc ccgcaaattt atcacttgtt tacctctgga
     5341 ggagagaaca tatgggctca actccaaccc ttgggagcaa tataacaaaa aacatgttat
     5401 ggtgccatta aaccgctgca tttcatcaaa gtcaagttga ttacctttac attttgatcc
     5461 tcttggatgt gaaaaaaact attaacatcc ctcaaaagac cccggttacg gccgccacca
     5521 tggactgtct ctgtatagtg acaacccggc gcgccgagca aaagctgata tctgaagagg
     5581 acttggggcg cgccaagaaa taccgctacc aagatgaaga cacgccccct ctggaacaca
     5641 gcccggccca cctccccaac caggccaatt ctccccctgt gattgtcaac acggacaccc
     5701 tagaagcccc aggatatgag ttgcaggtga atggaacaga gggggagatg gagtatgagg
     5761 agatcacatt ggaaaggggt aactcaggtc tgggcttcag catcgcaggt ggcactgaca
     5821 acccgcacat cggtgacgac ccgtccattt ttatcaccaa gatcattcct ggtggggctg
     5881 cagcccagga tggccgcctc agggtcaatg acagcatcct gtttgtaaat gaagtggatg
     5941 ttcgggaggt gacccattca gctgcggtgg aggccctcaa agaggcaggt tccatcgttc
     6001 gccactatgt catgcgccgg aaacccccag ccgaaaaggt catggagatc aaactcatca
     6061 aagggcctaa aggacttggc ttcagcattg cggggggcgt tgggaaccag cacatccctg
     6121 gagataacag catctatgta acgaagatca tcgaaggagg tgctgctcac aaggatggca
     6181 ggttgcagat tggagacaag atcctggcgg tcaacagtgt ggggctggag gacgttatgc
     6241 acgaggatgc cgtggcagcc ctgaagaaca catatgacgt tgtgtaccta aaggtggcca
     6301 agcccagcaa tgcctacctg agtgacagct atgctccccc agacatcaca acctcgtatt
     6361 ctcagcacct ggacaatgag atcagtcata gcagctactt gggcactgac taccccacag
     6421 ccatgacccc cacttcccct cggcgctact cccctgtggc caaggacctg ctgggggagg
     6481 aagacattcc ccgggaacca aggcggatcg tgatccatcg gggctccacc ggcctgggct
     6541 tcaacatcgt gggcggcgag gatggtgaag gcatcttcat ctccttcatc cttgctgggg
     6601 gtccagccga cctcagtggg gagctacgga agggggacca gatcctgtcg gtcaatggtg
     6661 ttgacctccg caatgccagt cacgaacagg ctgccattgc cctgaagaat gcgggtcaga
     6721 cggtcacgat catcgctcag tataaaccag aagagtatag tcgattcgag gccaagatcc
     6781 atgatcttcg ggaacagctc atgaatagta gcctaggctc agggactgca tccttgcgaa
     6841 gcaaccccaa gaggggcttc tacattaggg ccctgtttga ttacgacaag accaaggact
     6901 gcggtttctt gagccaggcc ctgagcttcc gcttcgggga tgtgcttcat gtcattgacg
     6961 ttggtgacga agagtggtgg caagcacggc gggtccactc cgacagtgag accgacgaca
     7021 ttggcttcat tcccagcaaa cggcgggtcg agcgacgaga gtggtcaagg ttaaaggcca
     7081 aggactgggg ctccagctct ggatcacagg gtcgagaaga ctcggttctg agctatgaga
     7141 cggtgaccca gatggaagtg cactatgctc gtcccatcat catccttgga cccaccaaag
     7201 accgtgccaa cgatgatctt ctctccgagt tccccgacaa gtttggatcc tgtgtccctc
     7261 atacgacacg tcctaagcgg gaatatgaga tagacggcca ggattaccac tttgtctcct
     7321 cccgggagaa aatggagaag gacatccagg cacacaagtt cattgaggct ggccagtaca
     7381 acagccacct ctatgggacc agcgtccagt ctgtgcgaga ggtagcagag caggggaagc
     7441 actgcatcct cgatgtctcg gccaatgccg tgcggcggct gcaggcggcc cacctgcacc
     7501 ccatcgccat cttcatccgt ccccgctccc tggagaatgt gctagagatc aataagcgga
     7561 tcacagagga gcaagcccgg aaagccttcg acagagccac gaagctggag caggagttca
     7621 cagagtgctt ctcagccatc gtagagggcg acagctttga agagatctat cacaaagtga
     7681 aacgtgtcat tgaagacctc tcaggcccct acatctgggt cccagcccga gagagactcc
     7741 cgcggatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     7801 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     7861 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     7921 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     7981 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     8041 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     8101 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     8161 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     8221 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     8281 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     8341 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     8401 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     8461 agtaagctag cttataaagt gctgggtcat ctaagctttt cagtcgagaa aaaaacatta
     8521 gatcagaaga acaactggca acacttctca acctgagact tacttcaaga tgctcgatcc
     8581 tggagaggtc tatgatgacc ctattgaccc aatcgagtta gaggctgaac ccagaggaac
     8641 ccccattgtc cccaacatct tgaggaactc tgactacaat ctcaactctc ctttgataga
     8701 agatcctgct agactaatgt tagaatggtt aaaaacaggg aatagacctt atcggatgac
     8761 tctaacagac aattgctcca ggtctttcag agttttgaaa gattatttca agaaggtaga
     8821 tttgggttct ctcaaggtgg gcggaatggc tgcacagtca atgatttctc tctggttata
     8881 tggtgcccac tctgaatcca acaggagccg gagatgtata acagacttgg cccatttcta
     8941 ttccaagtcg tcccccatag agaagctgtt gaatctcacg ctaggaaata gagggctgag
     9001 aatcccccca gagggagtgt taagttgcct tgagagggtt gattatgata atgcatttgg
     9061 aaggtatctt gccaacacgt attcctctta cttgttcttc catgtaatca ccttatacat
     9121 gaacgcccta gactgggatg aagaaaagac catcctagca ttatggaaag atttaacctc
     9181 agtggacatc gggaaggact tggtaaagtt caaagaccaa atatggggac tgctgatcgt
     9241 gacaaaggac tttgtttact cccaaagttc caattgtctt tttgacagaa actacacact
     9301 tatgctaaaa gatcttttct tgtctcgctt caactcctta atggtcttgc tctctccccc
     9361 agagccccga tactcagatg acttgatatc tcaactatgc cagctgtaca ttgctgggga
     9421 tcaagtcttg tctatgtgtg gaaactccgg ctatgaagtc atcaaaatat tggagccata
     9481 tgtcgtgaat agtttagtcc agagagcaga aaagtttagg cctctcattc attccttggg
     9541 agactttcct gtatttataa aagacaaggt aagtcaactt gaagagacgt tcggtccctg
     9601 tgcaagaagg ttctttaggg ctctggatca attcgacaac atacatgact tggtttttgt
     9661 gtttggctgt tacaggcatt gggggcaccc atatatagat tatcgaaagg gtctgtcaaa
     9721 actatatgat caggttcacc ttaaaaaaat gatagataag tcctaccagg agtgcttagc
     9781 aagcgaccta gccaggagga tccttagatg gggttttgat aagtactcca agtggtatct
     9841 ggattcaaga ttcctagccc gagaccaccc cttgactcct tatatcaaaa cccaaacatg
     9901 gccacccaaa catattgtag acttggtggg ggatacatgg cacaagctcc cgatcacgca
     9961 gatctttgag attcctgaat caatggatcc gtcagaaata ttggatgaca aatcacattc
    10021 tttcaccaga acgagactag cttcttggct gtcagaaaac cgaggggggc ctgttcctag
    10081 cgaaaaagtt attatcacgg ccctgtctaa gccgcctgtc aatccccgag agtttctgag
    10141 gtctatagac ctcggaggat tgccagatga agacttgata attggcctca agccaaagga
    10201 acgggaattg aagattgaag gtcgattctt tgctctaatg tcatggaatc taagattgta
    10261 ttttgtcatc actgaaaaac tcttggccaa ctacatcttg ccactttttg acgcgctgac
    10321 tatgacagac aacctgaaca aggtgtttaa aaagctgatc gacagggtca ccgggcaagg
    10381 gcttttggac tattcaaggg tcacatatgc atttcacctg gactatgaaa agtggaacaa
    10441 ccatcaaaga ttagagtcaa cagaggatgt attttctgtc ctagatcaag tgtttggatt
    10501 gaagagagtg ttttctagaa cacacgagtt ttttcaaaag gcctggatct attattcaga
    10561 cagatcagac ctcatcgggt tacgggagga tcaaatatac tgcttagatg cgtccaacgg
    10621 cccaacctgt tggaatggcc aggatggcgg gctagaaggc ttacggcaga agggctggag
    10681 tctagtcagc ttattgatga tagatagaga atctcaaatc aggaacacaa gaaccaaaat
    10741 actagctcaa ggagacaacc aggttttatg tccgacatac atgttgtcgc cagggctatc
    10801 tcaagagggg ctcctctatg aattggagag aatatcaagg aatgcacttt cgatatacag
    10861 agccgtcgag gaaggggcat ctaagctagg gctgatcatc aagaaagaag agaccatgtg
    10921 tagttatgac ttcctcatct atggaaaaac ccctttgttt agaggtaaca tattggtgcc
    10981 tgagtccaaa agatgggcca gagtctcttg cgtctctaat gaccaaatag tcaacctcgc
    11041 caatataatg tcgacagtgt ccaccaatgc gctaacagtg gcacaacact ctcaatcttt
    11101 gatcaaaccg atgagggatt ttctgctcat gtcagtacag gcagtctttc actacctgct
    11161 atttagccca atcttaaagg gaagagttta caagattctg agcgctgaag gggagagctt
    11221 tctcctagcc atgtcaagga taatctatct agatccttct ttgggaggga tatctggaat
    11281 gtccctcgga agattccata tacgacagtt ctcagaccct gtctctgaag ggttatcctt
    11341 ctggagagag atctggttaa gctcccaaga gtcctggatt cacgcgttgt gtcaagaggc
    11401 tggaaaccca gatcttggag agagaacact cgagagcttc actcgccttc tagaagatcc
    11461 gaccacctta aatatcagag gaggggccag tcctaccatt ctactcaagg atgcaatcag
    11521 aaaggcttta tatgacgagg tggacaaggt ggaaaattca gagtttcgag aggcaatcct
    11581 gttgtccaag acccatagag ataattttat actcttctta atatctgttg agcctctgtt
    11641 tcctcgattt ctcagtgagc tattcagttc gtcttttttg ggaatccccg agtcaatcat
    11701 tggattgata caaaactccc gaacgataag aaggcagttt agaaagagtc tctcaaaaac
    11761 tttagaagaa tccttctaca actcagagat ccacgggatt agtcggatga cccagacacc
    11821 tcagagggtt gggggggtgt ggccttgctc ttcagagagg gcagatctac ttagggagat
    11881 ctcttgggga agaaaagtgg taggcacgac agttcctcac ccttctgaga tgttgggatt
    11941 acttcccaag tcctctattt cttgcacttg tggagcaaca ggaggaggca atcctagagt
    12001 ttctgtatca gtactcccgt cctttgatca gtcatttttt tcacgaggcc ccctaaaggg
    12061 atacttgggc tcgtccacct ctatgtcgac ccagctattc catgcatggg aaaaagtcac
    12121 taatgttcat gtggtgaaga gagctctatc gttaaaagaa tctataaact ggttcattac
    12181 tagagattcc aacttggctc aagctctaat taggaacatt atgtctctga caggccctga
    12241 tttccctcta gaggaggccc ctgtcttcaa aaggacgggg tcagccttgc ataggttcaa
    12301 gtctgccaga tacagcgaag gagggtattc ttctgtctgc ccgaacctcc tctctcatat
    12361 ttctgttagt acagacacca tgtctgattt gacccaagac gggaagaact acgatttcat
    12421 gttccagcca ttgatgcttt atgcacagac atggacatca gagctggtac agagagacac
    12481 aaggctaaga gactctacgt ttcattggca cctccgatgc aacaggtgtg tgagacccat
    12541 tgacgacgtg accctggaga cctctcagat cttcgagttt ccggatgtgt cgaaaagaat
    12601 atccagaatg gtttctgggg ctgtgcctca cttccagagg cttcccgata tccgtctgag
    12661 accaggagat tttgaatctc taagcggtag agaaaagtct caccatatcg gatcagctca
    12721 ggggctctta tactcaatct tagtggcaat tcacgactca ggatacaatg atggaaccat
    12781 cttccctgtc aacatatacg gcaaggtttc ccctagagac tatttgagag ggctcgcaag
    12841 gggagtattg ataggatcct cgatttgctt cttgacaaga atgacaaata tcaatattaa
    12901 tagacctctt gaattggtct caggggtaat ctcatatatt ctcctgaggc tagataacca
    12961 tccctccttg tacataatgc tcagagaacc gtctcttaga ggagagatat tttctatccc
    13021 tcagaaaatc cccgccgctt atccaaccac tatgaaagaa ggcaacagat caatcttgtg
    13081 ttatctccaa catgtgctac gctatgagcg agagataatc acggcgtctc cagagaatga
    13141 ctggctatgg atcttttcag actttagaag tgccaaaatg acgtacctat ccctcattac
    13201 ttaccagtct catcttctac tccagagggt tgagagaaac ctatctaaga gtatgagaga
    13261 taacctgcga caattgagtt ctttgatgag gcaggtgctg ggcgggcacg gagaagatac
    13321 cttagagtca gacgacaaca ttcaacgact gctaaaagac tctttacgaa ggacaagatg
    13381 ggtggatcaa gaggtgcgcc atgcagctag aaccatgact ggagattaca gccccaacaa
    13441 gaaggtgtcc cgtaaggtag gatgttcaga atgggtctgc tctgctcaac aggttgcagt
    13501 ctctacctca gcaaacccgg cccctgtctc ggagcttgac ataagggccc tctctaagag
    13561 gttccagaac cctttgatct cgggcttgag agtggttcag tgggcaaccg gtgctcatta
    13621 taagcttaag cctattctag atgatctcaa tgttttccca tctctctgcc ttgtagttgg
    13681 ggacgggtca ggggggatat caagggcagt cctcaacatg tttccagatg ccaagcttgt
    13741 gttcaacagt cttttagagg tgaatgacct gatggcttcc ggaacacatc cactgcctcc
    13801 ttcagcaatc atgaggggag gaaatgatat cgtctccaga gtgatagatc ttgactcaat
    13861 ctgggaaaaa ccgtccgact tgagaaactt ggcaacctgg aaatacttcc agtcagtcca
    13921 aaagcaggtc aacatgtcct atgacctcat tatttgcgat gcagaagtta ctgacattgc
    13981 atctatcaac cggatcaccc tgttaatgtc cgattttgca ttgtctatag atggaccact
    14041 ctatttggtc ttcaaaactt atgggactat gctagtaaat ccaaactaca aggctattca
    14101 acacctgtca agagcgttcc cctcggtcac agggtttatc acccaagtaa cttcgtcttt
    14161 ttcatctgag ctctacctcc gattctccaa acgagggaag tttttcagag atgctgagta
    14221 cttgacctct tccacccttc gagaaatgag ccttgtgtta ttcaattgta gcagccccaa
    14281 gagtgagatg cagagagctc gttccttgaa ctatcaggat cttgtgagag gatttcctga
    14341 agaaatcata tcaaatcctt acaatgagat gatcataact ctgattgaca gtgatgtaga
    14401 atcttttcta gtccacaaga tggttgatga tcttgagtta cagaggggaa ctctgtctaa
    14461 agtggctatc attatagcca tcatgatagt tttctccaac agagtcttca acgtttccaa
    14521 acccctaact gacccctcgt tctatccacc gtctgatccc aaaatcctga ggcacttcaa
    14581 catatgttgc agtactatga tgtatctatc tactgcttta ggtgacgtcc ctagcttcgc
    14641 aagacttcac gacctgtata acagacctat aacttattac ttcagaaagc aagtcattcg
    14701 agggaacgtt tatctatctt ggagttggtc caacgacacc tcagtgttca aaagggtagc
    14761 ctgtaattct agcctgagtc tgtcatctca ctggatcagg ttgatttaca agatagtgaa
    14821 gactaccaga ctcgttggca gcatcaagga tctatccaga gaagtggaaa gacaccttca
    14881 taggtacaac aggtggatca ccctagagga tatcagatct agatcatccc tactagacta
    14941 cagttgcctg tgaaccggat actcctggaa gcctgcccat gctaagactc ttgtgtgatg
    15001 tatcttgaaa aaaacaagat cctaaatctg aacctttggt tgtttgattg tttttctcat
    15061 ttttgttgtt tatttgttaa gcgtgggtcg gcatggcatc tccacctcct cgcggtccga
    15121 cctgggcatc cgaaggagga cgcacgtcca ctcggatggc taagggaggg gcggggatcc
    15181 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
    15241 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
    15301 tatatccgga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
    15361 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
    15421 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    15481 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    15541 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    15601 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    15661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    15721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    15781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    15841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    15901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    15961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    16021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    16081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    16141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    16201 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    16261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    16321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    16381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    16441 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    16501 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    16561 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    16621 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    16681 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    16741 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    16801 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    16861 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    16921 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    16981 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    17041 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    17101 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    17161 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    17221 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    17281 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    17341 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    17401 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    17461 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    17521 ttccgcgcac atttccccga aaagtgccac ctg
//
