LOCUS       sequence-81376-e       16265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP and Synaptophysin-TagRFP-T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16265)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 16265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-N-P-M-EGFP-SynPhRFP-L
FEATURES             Location/Qualifiers
     source          1..16265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4695..4697,4698..4700,4701..5414)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4695..4697,4698..4700,4701..5411)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4740..4761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5001..5020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5348..5369
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      5478..5487
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6438..6447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6444..7175
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             8927..8938
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13937..13955)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13951..13998
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(14021..14037)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14021..14037)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(14074..14094)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14074..14092)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14113..14129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14113..14129)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14126..14148)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14137..14153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14161..14167,14168..14185,14186..14191))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14206..14227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14344..14361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14515..15103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14595..14614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15274..16065,16066..16134))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15897..15916
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16135..16239)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     4741 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     4801 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4861 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     4921 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4981 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     5041 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5101 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     5161 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     5221 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     5281 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     5341 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     5401 agctgtacaa gtaattaatt aacgtccttt caacgatcca agtccatgaa aaaaactaac
     5461 acccctcccg tacggccgcc accatggacg tggtgaatca gctggtggct gggggtcagt
     5521 tccgggtggt caaggagccc cttggcttcg tgaaggtgct gcagtgggtc tttgccatct
     5581 tcgcctttgc tacgtgcggc agctacaccg gagagcttcg gctgagcgtg gagtgtgcca
     5641 acaagacgga gagtgccctc aacatcgaag tcgaatttga gtacccattc aggctgcacc
     5701 aagtgtactt tgatgcaccc tcctgcgtta aagggggcac taccaagatc ttcctagttg
     5761 gtgactactc ctcctcgggt gaattctttg tcaccgtggc tgtgtttgcc ttcctctact
     5821 ccatgggggc cctggccacc tacatcttcc tgcagaacaa gtaccgagag aacaacaaag
     5881 ggccaatgat ggacttcctg gccacagcag tgttcgcttt catgtggcta gttagctcat
     5941 ccgcctgggc caaaggcctg tccgatgtga agatggccac tgacccagag aacattatca
     6001 aggagatgcc tatgtgccgc cagacaggaa acacatgcaa ggaactgagg gaccctgtga
     6061 cttcaggact caacacctcg gtggtgtttg gcttcctgaa cctggtgctc tgggttggca
     6121 acctatggtt cgtgttcaag gagacaggct gggccgcccc attcatgcgc gcacctccag
     6181 gcgccccaga aaagcaacca gctcctggcg atgcctacgg cgatgcgggc tatgggcagg
     6241 gccccggagg ctatgggccc caggactcct acgggcctca gggtggttat caacccgatt
     6301 acgggcagcc agccagcggc ggtggcggtg gctacgggcc tcagggcgac tatgggcagc
     6361 aaggctacgg ccaacagggt gcgcccacct ccttctccaa tcagatgggc ggaggccctg
     6421 ggatccaccg gccggtcgcc accatggtgt ctaagggcga agagctgatt aaggagaaca
     6481 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
     6541 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     6601 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcagaacct
     6661 tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct gagggcttca
     6721 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     6781 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca
     6841 acggccctgt gatgcagaag aaaacactcg gctgggaggc caacaccgag atgctgtacc
     6901 ccgctgacgg cggcctggaa ggcagaaccg acatggccct gaagctcgtg ggcgggggcc
     6961 acctgatctg caacttcaag accacataca gatccaagaa acccgctaag aacctcaaga
     7021 tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaggaggcc gacaaagaga
     7081 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
     7141 ggcacaaact taatggcatg gacgagctgt acaagtaagc tagcttataa agtgctgggt
     7201 catctaagct tttcagtcga gaaaaaaaca ttagatcaga agaacaactg gcaacacttc
     7261 tcaacctgag acttacttca agatgctcga tcctggagag gtctatgatg accctattga
     7321 cccaatcgag ttagaggctg aacccagagg aacccccatt gtccccaaca tcttgaggaa
     7381 ctctgactac aatctcaact ctcctttgat agaagatcct gctagactaa tgttagaatg
     7441 gttaaaaaca gggaatagac cttatcggat gactctaaca gacaattgct ccaggtcttt
     7501 cagagttttg aaagattatt tcaagaaggt agatttgggt tctctcaagg tgggcggaat
     7561 ggctgcacag tcaatgattt ctctctggtt atatggtgcc cactctgaat ccaacaggag
     7621 ccggagatgt ataacagact tggcccattt ctattccaag tcgtccccca tagagaagct
     7681 gttgaatctc acgctaggaa atagagggct gagaatcccc ccagagggag tgttaagttg
     7741 ccttgagagg gttgattatg ataatgcatt tggaaggtat cttgccaaca cgtattcctc
     7801 ttacttgttc ttccatgtaa tcaccttata catgaacgcc ctagactggg atgaagaaaa
     7861 gaccatccta gcattatgga aagatttaac ctcagtggac atcgggaagg acttggtaaa
     7921 gttcaaagac caaatatggg gactgctgat cgtgacaaag gactttgttt actcccaaag
     7981 ttccaattgt ctttttgaca gaaactacac acttatgcta aaagatcttt tcttgtctcg
     8041 cttcaactcc ttaatggtct tgctctctcc cccagagccc cgatactcag atgacttgat
     8101 atctcaacta tgccagctgt acattgctgg ggatcaagtc ttgtctatgt gtggaaactc
     8161 cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg aatagtttag tccagagagc
     8221 agaaaagttt aggcctctca ttcattcctt gggagacttt cctgtattta taaaagacaa
     8281 ggtaagtcaa cttgaagaga cgttcggtcc ctgtgcaaga aggttcttta gggctctgga
     8341 tcaattcgac aacatacatg acttggtttt tgtgtttggc tgttacaggc attgggggca
     8401 cccatatata gattatcgaa agggtctgtc aaaactatat gatcaggttc accttaaaaa
     8461 aatgatagat aagtcctacc aggagtgctt agcaagcgac ctagccagga ggatccttag
     8521 atggggtttt gataagtact ccaagtggta tctggattca agattcctag cccgagacca
     8581 ccccttgact ccttatatca aaacccaaac atggccaccc aaacatattg tagacttggt
     8641 gggggataca tggcacaagc tcccgatcac gcagatcttt gagattcctg aatcaatgga
     8701 tccgtcagaa atattggatg acaaatcaca ttctttcacc agaacgagac tagcttcttg
     8761 gctgtcagaa aaccgagggg ggcctgttcc tagcgaaaaa gttattatca cggccctgtc
     8821 taagccgcct gtcaatcccc gagagtttct gaggtctata gacctcggag gattgccaga
     8881 tgaagacttg ataattggcc tcaagccaaa ggaacgggaa ttgaagattg aaggtcgatt
     8941 ctttgctcta atgtcatgga atctaagatt gtattttgtc atcactgaaa aactcttggc
     9001 caactacatc ttgccacttt ttgacgcgct gactatgaca gacaacctga acaaggtgtt
     9061 taaaaagctg atcgacaggg tcaccgggca agggcttttg gactattcaa gggtcacata
     9121 tgcatttcac ctggactatg aaaagtggaa caaccatcaa agattagagt caacagagga
     9181 tgtattttct gtcctagatc aagtgtttgg attgaagaga gtgttttcta gaacacacga
     9241 gttttttcaa aaggcctgga tctattattc agacagatca gacctcatcg ggttacggga
     9301 ggatcaaata tactgcttag atgcgtccaa cggcccaacc tgttggaatg gccaggatgg
     9361 cgggctagaa ggcttacggc agaagggctg gagtctagtc agcttattga tgatagatag
     9421 agaatctcaa atcaggaaca caagaaccaa aatactagct caaggagaca accaggtttt
     9481 atgtccgaca tacatgttgt cgccagggct atctcaagag gggctcctct atgaattgga
     9541 gagaatatca aggaatgcac tttcgatata cagagccgtc gaggaagggg catctaagct
     9601 agggctgatc atcaagaaag aagagaccat gtgtagttat gacttcctca tctatggaaa
     9661 aacccctttg tttagaggta acatattggt gcctgagtcc aaaagatggg ccagagtctc
     9721 ttgcgtctct aatgaccaaa tagtcaacct cgccaatata atgtcgacag tgtccaccaa
     9781 tgcgctaaca gtggcacaac actctcaatc tttgatcaaa ccgatgaggg attttctgct
     9841 catgtcagta caggcagtct ttcactacct gctatttagc ccaatcttaa agggaagagt
     9901 ttacaagatt ctgagcgctg aaggggagag ctttctccta gccatgtcaa ggataatcta
     9961 tctagatcct tctttgggag ggatatctgg aatgtccctc ggaagattcc atatacgaca
    10021 gttctcagac cctgtctctg aagggttatc cttctggaga gagatctggt taagctccca
    10081 agagtcctgg attcacgcgt tgtgtcaaga ggctggaaac ccagatcttg gagagagaac
    10141 actcgagagc ttcactcgcc ttctagaaga tccgaccacc ttaaatatca gaggaggggc
    10201 cagtcctacc attctactca aggatgcaat cagaaaggct ttatatgacg aggtggacaa
    10261 ggtggaaaat tcagagtttc gagaggcaat cctgttgtcc aagacccata gagataattt
    10321 tatactcttc ttaatatctg ttgagcctct gtttcctcga tttctcagtg agctattcag
    10381 ttcgtctttt ttgggaatcc ccgagtcaat cattggattg atacaaaact cccgaacgat
    10441 aagaaggcag tttagaaaga gtctctcaaa aactttagaa gaatccttct acaactcaga
    10501 gatccacggg attagtcgga tgacccagac acctcagagg gttggggggg tgtggccttg
    10561 ctcttcagag agggcagatc tacttaggga gatctcttgg ggaagaaaag tggtaggcac
    10621 gacagttcct cacccttctg agatgttggg attacttccc aagtcctcta tttcttgcac
    10681 ttgtggagca acaggaggag gcaatcctag agtttctgta tcagtactcc cgtcctttga
    10741 tcagtcattt ttttcacgag gccccctaaa gggatacttg ggctcgtcca cctctatgtc
    10801 gacccagcta ttccatgcat gggaaaaagt cactaatgtt catgtggtga agagagctct
    10861 atcgttaaaa gaatctataa actggttcat tactagagat tccaacttgg ctcaagctct
    10921 aattaggaac attatgtctc tgacaggccc tgatttccct ctagaggagg cccctgtctt
    10981 caaaaggacg gggtcagcct tgcataggtt caagtctgcc agatacagcg aaggagggta
    11041 ttcttctgtc tgcccgaacc tcctctctca tatttctgtt agtacagaca ccatgtctga
    11101 tttgacccaa gacgggaaga actacgattt catgttccag ccattgatgc tttatgcaca
    11161 gacatggaca tcagagctgg tacagagaga cacaaggcta agagactcta cgtttcattg
    11221 gcacctccga tgcaacaggt gtgtgagacc cattgacgac gtgaccctgg agacctctca
    11281 gatcttcgag tttccggatg tgtcgaaaag aatatccaga atggtttctg gggctgtgcc
    11341 tcacttccag aggcttcccg atatccgtct gagaccagga gattttgaat ctctaagcgg
    11401 tagagaaaag tctcaccata tcggatcagc tcaggggctc ttatactcaa tcttagtggc
    11461 aattcacgac tcaggataca atgatggaac catcttccct gtcaacatat acggcaaggt
    11521 ttcccctaga gactatttga gagggctcgc aaggggagta ttgataggat cctcgatttg
    11581 cttcttgaca agaatgacaa atatcaatat taatagacct cttgaattgg tctcaggggt
    11641 aatctcatat attctcctga ggctagataa ccatccctcc ttgtacataa tgctcagaga
    11701 accgtctctt agaggagaga tattttctat ccctcagaaa atccccgccg cttatccaac
    11761 cactatgaaa gaaggcaaca gatcaatctt gtgttatctc caacatgtgc tacgctatga
    11821 gcgagagata atcacggcgt ctccagagaa tgactggcta tggatctttt cagactttag
    11881 aagtgccaaa atgacgtacc tatccctcat tacttaccag tctcatcttc tactccagag
    11941 ggttgagaga aacctatcta agagtatgag agataacctg cgacaattga gttctttgat
    12001 gaggcaggtg ctgggcgggc acggagaaga taccttagag tcagacgaca acattcaacg
    12061 actgctaaaa gactctttac gaaggacaag atgggtggat caagaggtgc gccatgcagc
    12121 tagaaccatg actggagatt acagccccaa caagaaggtg tcccgtaagg taggatgttc
    12181 agaatgggtc tgctctgctc aacaggttgc agtctctacc tcagcaaacc cggcccctgt
    12241 ctcggagctt gacataaggg ccctctctaa gaggttccag aaccctttga tctcgggctt
    12301 gagagtggtt cagtgggcaa ccggtgctca ttataagctt aagcctattc tagatgatct
    12361 caatgttttc ccatctctct gccttgtagt tggggacggg tcagggggga tatcaagggc
    12421 agtcctcaac atgtttccag atgccaagct tgtgttcaac agtcttttag aggtgaatga
    12481 cctgatggct tccggaacac atccactgcc tccttcagca atcatgaggg gaggaaatga
    12541 tatcgtctcc agagtgatag atcttgactc aatctgggaa aaaccgtccg acttgagaaa
    12601 cttggcaacc tggaaatact tccagtcagt ccaaaagcag gtcaacatgt cctatgacct
    12661 cattatttgc gatgcagaag ttactgacat tgcatctatc aaccggatca ccctgttaat
    12721 gtccgatttt gcattgtcta tagatggacc actctatttg gtcttcaaaa cttatgggac
    12781 tatgctagta aatccaaact acaaggctat tcaacacctg tcaagagcgt tcccctcggt
    12841 cacagggttt atcacccaag taacttcgtc tttttcatct gagctctacc tccgattctc
    12901 caaacgaggg aagtttttca gagatgctga gtacttgacc tcttccaccc ttcgagaaat
    12961 gagccttgtg ttattcaatt gtagcagccc caagagtgag atgcagagag ctcgttcctt
    13021 gaactatcag gatcttgtga gaggatttcc tgaagaaatc atatcaaatc cttacaatga
    13081 gatgatcata actctgattg acagtgatgt agaatctttt ctagtccaca agatggttga
    13141 tgatcttgag ttacagaggg gaactctgtc taaagtggct atcattatag ccatcatgat
    13201 agttttctcc aacagagtct tcaacgtttc caaaccccta actgacccct cgttctatcc
    13261 accgtctgat cccaaaatcc tgaggcactt caacatatgt tgcagtacta tgatgtatct
    13321 atctactgct ttaggtgacg tccctagctt cgcaagactt cacgacctgt ataacagacc
    13381 tataacttat tacttcagaa agcaagtcat tcgagggaac gtttatctat cttggagttg
    13441 gtccaacgac acctcagtgt tcaaaagggt agcctgtaat tctagcctga gtctgtcatc
    13501 tcactggatc aggttgattt acaagatagt gaagactacc agactcgttg gcagcatcaa
    13561 ggatctatcc agagaagtgg aaagacacct tcataggtac aacaggtgga tcaccctaga
    13621 ggatatcaga tctagatcat ccctactaga ctacagttgc ctgtgaaccg gatactcctg
    13681 gaagcctgcc catgctaaga ctcttgtgtg atgtatcttg aaaaaaacaa gatcctaaat
    13741 ctgaaccttt ggttgtttga ttgtttttct catttttgtt gtttatttgt taagcgtggg
    13801 tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgcacgt
    13861 ccactcggat ggctaaggga gggcggggat ccggctgcta acaaagcccg aaaggaagct
    13921 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
    13981 gtcttgaggg gttttttgct gaaaggagga actatatccg gatccactag ttctagagcg
    14041 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
    14101 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    14161 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    14221 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    14281 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    14341 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    14401 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    14461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    14521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    14581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    14641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    14701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    14761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    14821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    14881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    14941 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    15001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    15061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    15121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    15181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    15241 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    15301 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    15361 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    15421 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    15481 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    15541 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    15601 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    15661 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    15721 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    15781 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    15841 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    15901 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    15961 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    16021 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    16081 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    16141 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    16201 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    16261 acctg
//
