LOCUS       sequence-81377-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Phamret, a monomeric photoconvertible 
            fluorescent protein, fused to H2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Matsuda T, Miyawaki A, Nagai T
  TITLE     Direct measurement of protein dynamics inside cells using a 
            rationally designed photoconvertible protein.
  JOURNAL   Nat Methods. 2008 Apr;5(4):339-45. doi: 10.1038/nmeth.1193. Epub 
            2008 Mar 16.
  PUBMED    18345008
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1193"; journalName: "Nat Methods"; date: "2008-04"; 
            volume: "5"; issue: "4"; pages: "339-45"
COMMENT     Sequence Label: Phamret-H2B/pcDNA3
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(93..95,96..98,99..611)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(399..418)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     746..767
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctt ggtaccgagc tcggatccca ccatggtgag caagggcgag gagctgttca
      121 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aggttcagcg
      181 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      241 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc
      301 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      361 ccgaaggcta cgtccaggag cgtaccatct tcttcaagga cgacggcaac tacaagaccc
      421 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      481 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca
      541 acgtctatat caccgccgac aagcagaaga acggcatcaa ggcccacttc aagatccgcc
      601 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      661 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
      721 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggta
      781 ccgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      841 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      901 tgaagt
//
