LOCUS       sequence-81378-e       16211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry, nuclear mTagBFP and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Axonal and subcellular labelling using modified rabies viral 
            vectors.
  JOURNAL   Nat Commun. 2013;4:2332. doi: 10.1038/ncomms3332.
  PUBMED    23945836
REFERENCE   2  (bases 1 to 16211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2332. doi"; pages: " 
            10.1038/ncomms3332"
COMMENT     Sequence Label: pRVdG-mCherry-N-P-M-nucBFP-EGFP-L
FEATURES             Location/Qualifiers
     source          1..16211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1453..1462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1459..2169
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1604..1622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1872..1891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5523..6221
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             6246..6266
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6270..6290
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6294..6314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6399..6408
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6405..6407,6408..6410,6411..7124)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6405..6407,6408..6410,6411..7121)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6450..6471)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6711..6730)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7058..7079
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8873..8884
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13883..13901)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13897..13944
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13967..13983)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13967..13983)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(14020..14040)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14020..14038)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14059..14075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14059..14075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14072..14094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14083..14099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14107..14113,14114..14131,14132..14137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14152..14173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14290..14307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14461..15049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14541..14560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15220..16011,16012..16080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15843..15862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16081..16185)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 ccggtgaaag ccgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     1501 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1561 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1621 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1681 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1741 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1801 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1861 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1921 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1981 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2041 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2101 atcgtggaac agtacgaacg cgccgagggc cgccactcta ccggcggcat ggacgagctg
     2161 tacaagtaat gacctaggga agttgaataa caaaatgccg gaaatctacg gattgtgtat
     2221 atccatcatg aaaaaaacta acacccctcc tttcgaacca tcccaaacat ggatgccgac
     2281 aagattgtat tcaaagtcaa taatcaggtg gtctctttga agcctgagat tatcgtggat
     2341 caatatgagt acaagtaccc tgccatcaaa gatttgaaaa agccctgtat aactctagga
     2401 aaggctcccg atttaaataa agcatacaag tcagttttgt caggcatgag cgccgccaaa
     2461 cttaatcctg acgatgtatg ttcctatttg gcagcggcaa tgcagttttt tgaggggaca
     2521 tgtccggaag actggaccag ctatggaatt gtgattgcac gaaaaggaga taagatcacc
     2581 ccaggttctc tggtggagat aaaacgtact gatgtagaag ggaattgggc tctgacagga
     2641 ggcatggaac tgacaagaga ccccactgtc cctgagcatg cgtccttagt cggtcttctc
     2701 ttgagtctgt ataggttgag caaaatatcc gggcaaaaca ctggtaacta taagacaaac
     2761 attgcagaca ggatagagca gatttttgag acagcccctt ttgttaaaat cgtggaacac
     2821 catactctaa tgacaactca caaaatgtgt gctaattgga gtactatacc aaacttcaga
     2881 tttttggccg gaacctatga catgtttttc tcccggattg agcatctata ttcagcaatc
     2941 agagtgggca cagttgtcac tgcttatgaa gactgttcag gactggtatc atttactggg
     3001 ttcataaaac aaatcaatct caccgctaga gaggcaatac tatatttctt ccacaagaac
     3061 tttgaggaag agataagaag aatgtttgag ccagggcagg agacagctgt tcctcactct
     3121 tatttcatcc acttccgttc actaggcttg agtgggaaat ctccttattc atcaaatgct
     3181 gttggtcacg tgttcaatct cattcacttt gtaggatgct atatgggtca agtcagatcc
     3241 ctaaatgcaa cggttattgc tgcatgtgct cctcatgaaa tgtctgttct agggggctat
     3301 ctgggagagg aattcttcgg gaaagggaca tttgaaagaa gattcttcag agatgagaaa
     3361 gaacttcaag aatacgaggc ggctgaactg acaaagactg acgtagcact ggcagatgat
     3421 ggaactgtca actctgacga cgaggactac ttttcaggtg aaaccagaag tccggaggct
     3481 gtttatactc gaatcatgat gaatggaggt cgactaaaga gatctcacat acggagatat
     3541 gtctcagtca gttccaatca tcaagcccgt ccaaactcat tcgccgagtt tctaaacaag
     3601 acatattcga gtgactcata agaagttgaa taacaaaatg ccggaaatct acggattgtg
     3661 tatatccatc atgaaaaaaa ctaacacccc tcctttcgaa ccatcccaaa catgagcaag
     3721 atctttgtca atcctagtgc tattagagcc ggtctggccg atcttgagat ggctgaagaa
     3781 actgttgatc tgatcaatag aaatatcgaa gacaatcagg ctcatctcca aggggaaccc
     3841 atagaggtgg acaatctccc tgaggatatg gggcgacttc acctggatga tggaaaatcg
     3901 cccaaccatg gtgagatagc caaggtggga gaaggcaagt atcgagagga ctttcagatg
     3961 gatgaaggag aggatcctag cttcctgttc cagtcatacc tggaaaatgt tggagtccaa
     4021 atagtcagac aaatgaggtc aggagagaga tttctcaaga tatggtcaca gaccgtagaa
     4081 gagattatat cctatgtcgc ggtcaacttt cccaaccctc caggaaagtc ttcagaggat
     4141 aaatcaaccc agactactgg ccgagagctc aagaaggaga caacacccac tccttctcag
     4201 agagaaagcc aatcatcgaa agccaggatg gcggctcaaa ttgcttctgg ccctccagcc
     4261 cttgaatggt cggctaccaa tgaagaggat gatctatcag tggaggctga gatcgctcac
     4321 cagattgcag aaagtttctc caaaaaatat aagtttccct ctcgatcctc agggatactc
     4381 ttgtataatt ttgagcaatt gaaaatgaac cttgatgata tagttaaaga ggcaaaaaat
     4441 gtaccaggtg tgacccgttt agcccatgac gggtccaaac tccccctaag atgtgtactg
     4501 ggatgggtcg ctttggccaa ctctaagaaa ttccagttgt tagtcgaatc cgacaagctg
     4561 agtaaaatca tgcaagatga cttgaatcgc tatacatctt gctaaccgaa cctctcccct
     4621 cagtccctct agacaataaa atccgagatg tcccaaagtc aacatgaaaa aaacaggcaa
     4681 caccactgat aaaatgaacc tcctacgtaa gatagtgaaa aaccgcaggg acgaggacac
     4741 tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat gacttgtggc ttccaccccc
     4801 tgaatacgtc ccgctgaaag aacttacagg caagaagaac atgaggaact tttgtatcaa
     4861 cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc aggatcctgc ggcacattct
     4921 gaaatcattc gacgagatat attctgggaa tcataggatg atcgggttag tcaaagtggt
     4981 tattggactg gctttgtcag gatctccagt ccctgagggc ctgaactggg tatacaaatt
     5041 gaggagaacc tttatcttcc agtgggctga ttccaggggc cctcttgaag gggaggagtt
     5101 ggaatactct caggagatca cttgggatga tgatactgag ttcgtcggat tgcaaataag
     5161 agtgattgca aaacagtgtc atatccaggg cagagtctgg tgtatcaaca tgaacccgag
     5221 agcatgtcaa ctatggtctg acatgtctct tcagacacaa aggtccgaag aggacaaaga
     5281 ttcctctctg cttctagaat aatcagatta tatcccgcaa atttatcact tgtttacctc
     5341 tggaggagag aacatatggg ctcaactcca acccttggga gcaatataac aaagaacatg
     5401 ttatggtgcc attaaaccgc tgcatttcat caaagtcaag ttgattacct ttacattttg
     5461 atcctcttgg atgtgaaaaa aactattaac atccctcaaa agaccccggg aaagccgcca
     5521 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     5581 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     5641 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     5701 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     5761 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     5821 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     5881 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     5941 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     6001 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
     6061 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     6121 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     6181 gatactgcga cctccctagc aaactggggc acaagcttaa ttccggactc agatctcgag
     6241 ctgatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta gatccaaaaa
     6301 agaagagaaa ggtaggatcc accggatcta gataattaat taacgtcctt tcaacgatcc
     6361 aagtccatga aaaaaactaa cacccctccc gtacggccgc caccatggtg agcaagggcg
     6421 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     6481 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     6541 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     6601 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     6661 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     6721 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     6781 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     6841 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     6901 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     6961 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     7021 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     7081 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagctagc ttataaagtg
     7141 ctgggtcatc taagcttttc agtcgagaaa aaaacattag atcagaagaa caactggcaa
     7201 cacttctcaa cctgagactt acttcaagat gctcgatcct ggagaggtct atgatgaccc
     7261 tattgaccca atcgagttag aggctgaacc cagaggaacc cccattgtcc ccaacatctt
     7321 gaggaactct gactacaatc tcaactctcc tttgatagaa gatcctgcta gactaatgtt
     7381 agaatggtta aaaacaggga atagacctta tcggatgact ctaacagaca attgctccag
     7441 gtctttcaga gttttgaaag attatttcaa gaaggtagat ttgggttctc tcaaggtggg
     7501 cggaatggct gcacagtcaa tgatttctct ctggttatat ggtgcccact ctgaatccaa
     7561 caggagccgg agatgtataa cagacttggc ccatttctat tccaagtcgt cccccataga
     7621 gaagctgttg aatctcacgc taggaaatag agggctgaga atccccccag agggagtgtt
     7681 aagttgcctt gagagggttg attatgataa tgcatttgga aggtatcttg ccaacacgta
     7741 ttcctcttac ttgttcttcc atgtaatcac cttatacatg aacgccctag actgggatga
     7801 agaaaagacc atcctagcat tatggaaaga tttaacctca gtggacatcg ggaaggactt
     7861 ggtaaagttc aaagaccaaa tatggggact gctgatcgtg acaaaggact ttgtttactc
     7921 ccaaagttcc aattgtcttt ttgacagaaa ctacacactt atgctaaaag atcttttctt
     7981 gtctcgcttc aactccttaa tggtcttgct ctctccccca gagccccgat actcagatga
     8041 cttgatatct caactatgcc agctgtacat tgctggggat caagtcttgt ctatgtgtgg
     8101 aaactccggc tatgaagtca tcaaaatatt ggagccatat gtcgtgaata gtttagtcca
     8161 gagagcagaa aagtttaggc ctctcattca ttccttggga gactttcctg tatttataaa
     8221 agacaaggta agtcaacttg aagagacgtt cggtccctgt gcaagaaggt tctttagggc
     8281 tctggatcaa ttcgacaaca tacatgactt ggtttttgtg tttggctgtt acaggcattg
     8341 ggggcaccca tatatagatt atcgaaaggg tctgtcaaaa ctatatgatc aggttcacct
     8401 taaaaaaatg atagataagt cctaccagga gtgcttagca agcgacctag ccaggaggat
     8461 ccttagatgg ggttttgata agtactccaa gtggtatctg gattcaagat tcctagcccg
     8521 agaccacccc ttgactcctt atatcaaaac ccaaacatgg ccacccaaac atattgtaga
     8581 cttggtgggg gatacatggc acaagctccc gatcacgcag atctttgaga ttcctgaatc
     8641 aatggatccg tcagaaatat tggatgacaa atcacattct ttcaccagaa cgagactagc
     8701 ttcttggctg tcagaaaacc gaggggggcc tgttcctagc gaaaaagtta ttatcacggc
     8761 cctgtctaag ccgcctgtca atccccgaga gtttctgagg tctatagacc tcggaggatt
     8821 gccagatgaa gacttgataa ttggcctcaa gccaaaggaa cgggaattga agattgaagg
     8881 tcgattcttt gctctaatgt catggaatct aagattgtat tttgtcatca ctgaaaaact
     8941 cttggccaac tacatcttgc cactttttga cgcgctgact atgacagaca acctgaacaa
     9001 ggtgtttaaa aagctgatcg acagggtcac cgggcaaggg cttttggact attcaagggt
     9061 cacatatgca tttcacctgg actatgaaaa gtggaacaac catcaaagat tagagtcaac
     9121 agaggatgta ttttctgtcc tagatcaagt gtttggattg aagagagtgt tttctagaac
     9181 acacgagttt tttcaaaagg cctggatcta ttattcagac agatcagacc tcatcgggtt
     9241 acgggaggat caaatatact gcttagatgc gtccaacggc ccaacctgtt ggaatggcca
     9301 ggatggcggg ctagaaggct tacggcagaa gggctggagt ctagtcagct tattgatgat
     9361 agatagagaa tctcaaatca ggaacacaag aaccaaaata ctagctcaag gagacaacca
     9421 ggttttatgt ccgacataca tgttgtcgcc agggctatct caagaggggc tcctctatga
     9481 attggagaga atatcaagga atgcactttc gatatacaga gccgtcgagg aaggggcatc
     9541 taagctaggg ctgatcatca agaaagaaga gaccatgtgt agttatgact tcctcatcta
     9601 tggaaaaacc cctttgttta gaggtaacat attggtgcct gagtccaaaa gatgggccag
     9661 agtctcttgc gtctctaatg accaaatagt caacctcgcc aatataatgt cgacagtgtc
     9721 caccaatgcg ctaacagtgg cacaacactc tcaatctttg atcaaaccga tgagggattt
     9781 tctgctcatg tcagtacagg cagtctttca ctacctgcta tttagcccaa tcttaaaggg
     9841 aagagtttac aagattctga gcgctgaagg ggagagcttt ctcctagcca tgtcaaggat
     9901 aatctatcta gatccttctt tgggagggat atctggaatg tccctcggaa gattccatat
     9961 acgacagttc tcagaccctg tctctgaagg gttatccttc tggagagaga tctggttaag
    10021 ctcccaagag tcctggattc acgcgttgtg tcaagaggct ggaaacccag atcttggaga
    10081 gagaacactc gagagcttca ctcgccttct agaagatccg accaccttaa atatcagagg
    10141 aggggccagt cctaccattc tactcaagga tgcaatcaga aaggctttat atgacgaggt
    10201 ggacaaggtg gaaaattcag agtttcgaga ggcaatcctg ttgtccaaga cccatagaga
    10261 taattttata ctcttcttaa tatctgttga gcctctgttt cctcgatttc tcagtgagct
    10321 attcagttcg tcttttttgg gaatccccga gtcaatcatt ggattgatac aaaactcccg
    10381 aacgataaga aggcagttta gaaagagtct ctcaaaaact ttagaagaat ccttctacaa
    10441 ctcagagatc cacgggatta gtcggatgac ccagacacct cagagggttg ggggggtgtg
    10501 gccttgctct tcagagaggg cagatctact tagggagatc tcttggggaa gaaaagtggt
    10561 aggcacgaca gttcctcacc cttctgagat gttgggatta cttcccaagt cctctatttc
    10621 ttgcacttgt ggagcaacag gaggaggcaa tcctagagtt tctgtatcag tactcccgtc
    10681 ctttgatcag tcattttttt cacgaggccc cctaaaggga tacttgggct cgtccacctc
    10741 tatgtcgacc cagctattcc atgcatggga aaaagtcact aatgttcatg tggtgaagag
    10801 agctctatcg ttaaaagaat ctataaactg gttcattact agagattcca acttggctca
    10861 agctctaatt aggaacatta tgtctctgac aggccctgat ttccctctag aggaggcccc
    10921 tgtcttcaaa aggacggggt cagccttgca taggttcaag tctgccagat acagcgaagg
    10981 agggtattct tctgtctgcc cgaacctcct ctctcatatt tctgttagta cagacaccat
    11041 gtctgatttg acccaagacg ggaagaacta cgatttcatg ttccagccat tgatgcttta
    11101 tgcacagaca tggacatcag agctggtaca gagagacaca aggctaagag actctacgtt
    11161 tcattggcac ctccgatgca acaggtgtgt gagacccatt gacgacgtga ccctggagac
    11221 ctctcagatc ttcgagtttc cggatgtgtc gaaaagaata tccagaatgg tttctggggc
    11281 tgtgcctcac ttccagaggc ttcccgatat ccgtctgaga ccaggagatt ttgaatctct
    11341 aagcggtaga gaaaagtctc accatatcgg atcagctcag gggctcttat actcaatctt
    11401 agtggcaatt cacgactcag gatacaatga tggaaccatc ttccctgtca acatatacgg
    11461 caaggtttcc cctagagact atttgagagg gctcgcaagg ggagtattga taggatcctc
    11521 gatttgcttc ttgacaagaa tgacaaatat caatattaat agacctcttg aattggtctc
    11581 aggggtaatc tcatatattc tcctgaggct agataaccat ccctccttgt acataatgct
    11641 cagagaaccg tctcttagag gagagatatt ttctatccct cagaaaatcc ccgccgctta
    11701 tccaaccact atgaaagaag gcaacagatc aatcttgtgt tatctccaac atgtgctacg
    11761 ctatgagcga gagataatca cggcgtctcc agagaatgac tggctatgga tcttttcaga
    11821 ctttagaagt gccaaaatga cgtacctatc cctcattact taccagtctc atcttctact
    11881 ccagagggtt gagagaaacc tatctaagag tatgagagat aacctgcgac aattgagttc
    11941 tttgatgagg caggtgctgg gcgggcacgg agaagatacc ttagagtcag acgacaacat
    12001 tcaacgactg ctaaaagact ctttacgaag gacaagatgg gtggatcaag aggtgcgcca
    12061 tgcagctaga accatgactg gagattacag ccccaacaag aaggtgtccc gtaaggtagg
    12121 atgttcagaa tgggtctgct ctgctcaaca ggttgcagtc tctacctcag caaacccggc
    12181 ccctgtctcg gagcttgaca taagggccct ctctaagagg ttccagaacc ctttgatctc
    12241 gggcttgaga gtggttcagt gggcaaccgg tgctcattat aagcttaagc ctattctaga
    12301 tgatctcaat gttttcccat ctctctgcct tgtagttggg gacgggtcag gggggatatc
    12361 aagggcagtc ctcaacatgt ttccagatgc caagcttgtg ttcaacagtc ttttagaggt
    12421 gaatgacctg atggcttccg gaacacatcc actgcctcct tcagcaatca tgaggggagg
    12481 aaatgatatc gtctccagag tgatagatct tgactcaatc tgggaaaaac cgtccgactt
    12541 gagaaacttg gcaacctgga aatacttcca gtcagtccaa aagcaggtca acatgtccta
    12601 tgacctcatt atttgcgatg cagaagttac tgacattgca tctatcaacc ggatcaccct
    12661 gttaatgtcc gattttgcat tgtctataga tggaccactc tatttggtct tcaaaactta
    12721 tgggactatg ctagtaaatc caaactacaa ggctattcaa cacctgtcaa gagcgttccc
    12781 ctcggtcaca gggtttatca cccaagtaac ttcgtctttt tcatctgagc tctacctccg
    12841 attctccaaa cgagggaagt ttttcagaga tgctgagtac ttgacctctt ccacccttcg
    12901 agaaatgagc cttgtgttat tcaattgtag cagccccaag agtgagatgc agagagctcg
    12961 ttccttgaac tatcaggatc ttgtgagagg atttcctgaa gaaatcatat caaatcctta
    13021 caatgagatg atcataactc tgattgacag tgatgtagaa tcttttctag tccacaagat
    13081 ggttgatgat cttgagttac agaggggaac tctgtctaaa gtggctatca ttatagccat
    13141 catgatagtt ttctccaaca gagtcttcaa cgtttccaaa cccctaactg acccctcgtt
    13201 ctatccaccg tctgatccca aaatcctgag gcacttcaac atatgttgca gtactatgat
    13261 gtatctatct actgctttag gtgacgtccc tagcttcgca agacttcacg acctgtataa
    13321 cagacctata acttattact tcagaaagca agtcattcga gggaacgttt atctatcttg
    13381 gagttggtcc aacgacacct cagtgttcaa aagggtagcc tgtaattcta gcctgagtct
    13441 gtcatctcac tggatcaggt tgatttacaa gatagtgaag actaccagac tcgttggcag
    13501 catcaaggat ctatccagag aagtggaaag acaccttcat aggtacaaca ggtggatcac
    13561 cctagaggat atcagatcta gatcatccct actagactac agttgcctgt gaaccggata
    13621 ctcctggaag cctgcccatg ctaagactct tgtgtgatgt atcttgaaaa aaacaagatc
    13681 ctaaatctga acctttggtt gtttgattgt ttttctcatt tttgttgttt atttgttaag
    13741 cgtgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac
    13801 gcacgtccac tcggatggct aagggagggc ggggatccgg ctgctaacaa agcccgaaag
    13861 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
    13921 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc cactagttct
    13981 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
    14041 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    14101 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    14161 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    14221 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    14281 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    14341 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    14401 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    14461 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    14521 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    14581 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    14641 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    14701 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    14761 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    14821 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    14881 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    14941 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    15001 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    15061 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    15121 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    15181 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    15241 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    15301 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    15361 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    15421 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    15481 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    15541 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    15601 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    15661 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    15721 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    15781 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    15841 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    15901 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    15961 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    16021 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    16081 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    16141 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    16201 agtgccacct g
//
