LOCUS       sequence-81383-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive 
            Ca2+ indicator with Kd = 50 nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura
            T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T
  TITLE     Spontaneous network activity visualized by ultrasensitive Ca(2+) 
            indicators, yellow Cameleon-Nano.
  JOURNAL   Nat Methods. 2010 Sep;7(9):729-32. doi: 10.1038/nmeth.1488. Epub 
            2010 Aug 8.
  PUBMED    20693999
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1488"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "729-32"
COMMENT     Sequence Label: Yellow Cameleon-Nano50/pcDNA3
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..150
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     primer_bind     294..315
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(373..375,376..378,379..837)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(418..439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(679..698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagatatcg atggtgatgg ccaagtaaac tatgaagagt ttgtacaaat gatgacagca
       61 aaggggggct ccaagaggcg ctggaagaaa aacttcattg ccgtcagcgc tgccaaccgg
      121 ttcaagaaga tctccagctc cggggcactg gagctcatgg acggcggcgt gcagctcgcc
      181 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      241 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      301 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
      361 ggcagcggtg gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      481 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
      841 aagca
//
