LOCUS       sequence-81384-e       14497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14497)
  AUTHORS   Weible AP, Schwarcz L, Wickersham IR, Deblander L, Wu H, Callaway 
            EM, Seung HS, Kentros CG
  TITLE     Transgenic targeting of recombinant rabies virus reveals 
            monosynaptic connectivity of specific neurons.
  JOURNAL   J Neurosci. 2010 Dec 8;30(49):16509-13. doi: 
            10.1523/JNEUROSCI.2442-10.2010.
  PUBMED    21147990
REFERENCE   2  (bases 1 to 14497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2442-10"; journalName: "J Neurosci"; date: 
            "2010-12-8- 8"; volume: "30"; issue: "49"; pages: "16509-13"
COMMENT     Sequence Label: pRVdG-4mCherry
FEATURES             Location/Qualifiers
     source          1..14497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4690..4699
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4696..5406
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4841..4859)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5109..5128
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7158..7169
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12169..12187)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12183..12230
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12253..12269)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12253..12269)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12306..12326)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12306..12324)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12345..12361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12345..12361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12358..12380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12369..12385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12393..12399,12400..12417,12418..12423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12438..12459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12576..12593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12747..13335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12827..12846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13506..14297,14298..14366))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14129..14148
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14367..14471)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 gtgaaagccg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     4741 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     4801 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     4861 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     4921 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     4981 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     5041 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     5101 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     5161 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     5221 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     5281 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     5341 gtggaacagt acgaacgcgc cgagggccgc cactctaccg gcggcatgga cgagctgtac
     5401 aagtaatgac ctagcttata aagtgctggg tcatctaagc ttttcagtcg agaaaaaaac
     5461 attagatcag aagaacaact ggcaacactt ctcaacctga gacttacttc aagatgctcg
     5521 atcctggaga ggtctatgat gaccctattg acccaatcga gttagaggct gaacccagag
     5581 gaacccccat tgtccccaac atcttgagga actctgacta caatctcaac tctcctttga
     5641 tagaagatcc tgctagacta atgttagaat ggttaaaaac agggaataga ccttatcgga
     5701 tgactctaac agacaattgc tccaggtctt tcagagtttt gaaagattat ttcaagaagg
     5761 tagatttggg ttctctcaag gtgggcggaa tggctgcaca gtcaatgatt tctctctggt
     5821 tatatggtgc ccactctgaa tccaacagga gccggagatg tataacagac ttggcccatt
     5881 tctattccaa gtcgtccccc atagagaagc tgttgaatct cacgctagga aatagagggc
     5941 tgagaatccc cccagaggga gtgttaagtt gccttgagag ggttgattat gataatgcat
     6001 ttggaaggta tcttgccaac acgtattcct cttacttgtt cttccatgta atcaccttat
     6061 acatgaacgc cctagactgg gatgaagaaa agaccatcct agcattatgg aaagatttaa
     6121 cctcagtgga catcgggaag gacttggtaa agttcaaaga ccaaatatgg ggactgctga
     6181 tcgtgacaaa ggactttgtt tactcccaaa gttccaattg tctttttgac agaaactaca
     6241 cacttatgct aaaagatctt ttcttgtctc gcttcaactc cttaatggtc ttgctctctc
     6301 ccccagagcc ccgatactca gatgacttga tatctcaact atgccagctg tacattgctg
     6361 gggatcaagt cttgtctatg tgtggaaact ccggctatga agtcatcaaa atattggagc
     6421 catatgtcgt gaatagttta gtccagagag cagaaaagtt taggcctctc attcattcct
     6481 tgggagactt tcctgtattt ataaaagaca aggtaagtca acttgaagag acgttcggtc
     6541 cctgtgcaag aaggttcttt agggctctgg atcaattcga caacatacat gacttggttt
     6601 ttgtgtttgg ctgttacagg cattgggggc acccatatat agattatcga aagggtctgt
     6661 caaaactata tgatcaggtt caccttaaaa aaatgataga taagtcctac caggagtgct
     6721 tagcaagcga cctagccagg aggatcctta gatggggttt tgataagtac tccaagtggt
     6781 atctggattc aagattccta gcccgagacc accccttgac tccttatatc aaaacccaaa
     6841 catggccacc caaacatatt gtagacttgg tgggggatac atggcacaag ctcccgatca
     6901 cgcagatctt tgagattcct gaatcaatgg atccgtcaga aatattggat gacaaatcac
     6961 attctttcac cagaacgaga ctagcttctt ggctgtcaga aaaccgaggg gggcctgttc
     7021 ctagcgaaaa agttattatc acggccctgt ctaagccgcc tgtcaatccc cgagagtttc
     7081 tgaggtctat agacctcgga ggattgccag atgaagactt gataattggc ctcaagccaa
     7141 aggaacggga attgaagatt gaaggtcgat tctttgctct aatgtcatgg aatctaagat
     7201 tgtattttgt catcactgaa aaactcttgg ccaactacat cttgccactt tttgacgcgc
     7261 tgactatgac agacaacctg aacaaggtgt ttaaaaagct gatcgacagg gtcaccgggc
     7321 aagggctttt ggactattca agggtcacat atgcatttca cctggactat gaaaagtgga
     7381 acaaccatca aagattagag tcaacagagg atgtattttc tgtcctagat caagtgtttg
     7441 gattgaagag agtgttttct agaacacacg agttttttca aaaggcctgg atctattatt
     7501 cagacagatc agacctcatc gggttacggg aggatcaaat atactgctta gatgcgtcca
     7561 acggcccaac ctgttggaat ggccaggatg gcgggctaga aggcttacgg cagaagggct
     7621 ggagtctagt cagcttattg atgatagata gagaatctca aatcaggaac acaagaacca
     7681 aaatactagc tcaaggagac aaccaggttt tatgtccgac atacatgttg tcgccagggc
     7741 tatctcaaga ggggctcctc tatgaattgg agagaatatc aaggaatgca ctttcgatat
     7801 acagagccgt cgaggaaggg gcatctaagc tagggctgat catcaagaaa gaagagacca
     7861 tgtgtagtta tgacttcctc atctatggaa aaaccccttt gtttagaggt aacatattgg
     7921 tgcctgagtc caaaagatgg gccagagtct cttgcgtctc taatgaccaa atagtcaacc
     7981 tcgccaatat aatgtcgaca gtgtccacca atgcgctaac agtggcacaa cactctcaat
     8041 ctttgatcaa accgatgagg gattttctgc tcatgtcagt acaggcagtc tttcactacc
     8101 tgctatttag cccaatctta aagggaagag tttacaagat tctgagcgct gaaggggaga
     8161 gctttctcct agccatgtca aggataatct atctagatcc ttctttggga gggatatctg
     8221 gaatgtccct cggaagattc catatacgac agttctcaga ccctgtctct gaagggttat
     8281 ccttctggag agagatctgg ttaagctccc aagagtcctg gattcacgcg ttgtgtcaag
     8341 aggctggaaa cccagatctt ggagagagaa cactcgagag cttcactcgc cttctagaag
     8401 atccgaccac cttaaatatc agaggagggg ccagtcctac cattctactc aaggatgcaa
     8461 tcagaaaggc tttatatgac gaggtggaca aggtggaaaa ttcagagttt cgagaggcaa
     8521 tcctgttgtc caagacccat agagataatt ttatactctt cttaatatct gttgagcctc
     8581 tgtttcctcg atttctcagt gagctattca gttcgtcttt tttgggaatc cccgagtcaa
     8641 tcattggatt gatacaaaac tcccgaacga taagaaggca gtttagaaag agtctctcaa
     8701 aaactttaga agaatccttc tacaactcag agatccacgg gattagtcgg atgacccaga
     8761 cacctcagag ggttgggggg gtgtggcctt gctcttcaga gagggcagat ctacttaggg
     8821 agatctcttg gggaagaaaa gtggtaggca cgacagttcc tcacccttct gagatgttgg
     8881 gattacttcc caagtcctct atttcttgca cttgtggagc aacaggagga ggcaatccta
     8941 gagtttctgt atcagtactc ccgtcctttg atcagtcatt tttttcacga ggccccctaa
     9001 agggatactt gggctcgtcc acctctatgt cgacccagct attccatgca tgggaaaaag
     9061 tcactaatgt tcatgtggtg aagagagctc tatcgttaaa agaatctata aactggttca
     9121 ttactagaga ttccaacttg gctcaagctc taattaggaa cattatgtct ctgacaggcc
     9181 ctgatttccc tctagaggag gcccctgtct tcaaaaggac ggggtcagcc ttgcataggt
     9241 tcaagtctgc cagatacagc gaaggagggt attcttctgt ctgcccgaac ctcctctctc
     9301 atatttctgt tagtacagac accatgtctg atttgaccca agacgggaag aactacgatt
     9361 tcatgttcca gccattgatg ctttatgcac agacatggac atcagagctg gtacagagag
     9421 acacaaggct aagagactct acgtttcatt ggcacctccg atgcaacagg tgtgtgagac
     9481 ccattgacga cgtgaccctg gagacctctc agatcttcga gtttccggat gtgtcgaaaa
     9541 gaatatccag aatggtttct ggggctgtgc ctcacttcca gaggcttccc gatatccgtc
     9601 tgagaccagg agattttgaa tctctaagcg gtagagaaaa gtctcaccat atcggatcag
     9661 ctcaggggct cttatactca atcttagtgg caattcacga ctcaggatac aatgatggaa
     9721 ccatcttccc tgtcaacata tacggcaagg tttcccctag agactatttg agagggctcg
     9781 caaggggagt attgatagga tcctcgattt gcttcttgac aagaatgaca aatatcaata
     9841 ttaatagacc tcttgaattg gtctcagggg taatctcata tattctcctg aggctagata
     9901 accatccctc cttgtacata atgctcagag aaccgtctct tagaggagag atattttcta
     9961 tccctcagaa aatccccgcc gcttatccaa ccactatgaa agaaggcaac agatcaatct
    10021 tgtgttatct ccaacatgtg ctacgctatg agcgagagat aatcacggcg tctccagaga
    10081 atgactggct atggatcttt tcagacttta gaagtgccaa aatgacgtac ctatccctca
    10141 ttacttacca gtctcatctt ctactccaga gggttgagag aaacctatct aagagtatga
    10201 gagataacct gcgacaattg agttctttga tgaggcaggt gctgggcggg cacggagaag
    10261 ataccttaga gtcagacgac aacattcaac gactgctaaa agactcttta cgaaggacaa
    10321 gatgggtgga tcaagaggtg cgccatgcag ctagaaccat gactggagat tacagcccca
    10381 acaagaaggt gtcccgtaag gtaggatgtt cagaatgggt ctgctctgct caacaggttg
    10441 cagtctctac ctcagcaaac ccggcccctg tctcggagct tgacataagg gccctctcta
    10501 agaggttcca gaaccctttg atctcgggct tgagagtggt tcagtgggca accggtgctc
    10561 attataagct taagcctatt ctagatgatc tcaatgtttt cccatctctc tgccttgtag
    10621 ttggggacgg gtcagggggg atatcaaggg cagtcctcaa catgtttcca gatgccaagc
    10681 ttgtgttcaa cagtctttta gaggtgaatg acctgatggc ttccggaaca catccactgc
    10741 ctccttcagc aatcatgagg ggaggaaatg atatcgtctc cagagtgata gatcttgact
    10801 caatctggga aaaaccgtcc gacttgagaa acttggcaac ctggaaatac ttccagtcag
    10861 tccaaaagca ggtcaacatg tcctatgacc tcattatttg cgatgcagaa gttactgaca
    10921 ttgcatctat caaccggatc accctgttaa tgtccgattt tgcattgtct atagatggac
    10981 cactctattt ggtcttcaaa acttatggga ctatgctagt aaatccaaac tacaaggcta
    11041 ttcaacacct gtcaagagcg ttcccctcgg tcacagggtt tatcacccaa gtaacttcgt
    11101 ctttttcatc tgagctctac ctccgattct ccaaacgagg gaagtttttc agagatgctg
    11161 agtacttgac ctcttccacc cttcgagaaa tgagccttgt gttattcaat tgtagcagcc
    11221 ccaagagtga gatgcagaga gctcgttcct tgaactatca ggatcttgtg agaggatttc
    11281 ctgaagaaat catatcaaat ccttacaatg agatgatcat aactctgatt gacagtgatg
    11341 tagaatcttt tctagtccac aagatggttg atgatcttga gttacagagg ggaactctgt
    11401 ctaaagtggc tatcattata gccatcatga tagttttctc caacagagtc ttcaacgttt
    11461 ccaaacccct aactgacccc tcgttctatc caccgtctga tcccaaaatc ctgaggcact
    11521 tcaacatatg ttgcagtact atgatgtatc tatctactgc tttaggtgac gtccctagct
    11581 tcgcaagact tcacgacctg tataacagac ctataactta ttacttcaga aagcaagtca
    11641 ttcgagggaa cgtttatcta tcttggagtt ggtccaacga cacctcagtg ttcaaaaggg
    11701 tagcctgtaa ttctagcctg agtctgtcat ctcactggat caggttgatt tacaagatag
    11761 tgaagactac cagactcgtt ggcagcatca aggatctatc cagagaagtg gaaagacacc
    11821 ttcataggta caacaggtgg atcaccctag aggatatcag atctagatca tccctactag
    11881 actacagttg cctgtgaacc ggatactcct ggaagcctgc ccatgctaag actcttgtgt
    11941 gatgtatctt gaaaaaaaca agatcctaaa tctgaacctt tggttgtttg attgtttttc
    12001 tcatttttgt tgtttatttg ttaagcgtgg gtcggcatgg catctccacc tcctcgcggt
    12061 ccgacctggg catccgaagg aggacgcacg tccactcgga tggctaaggg aggggcgggg
    12121 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
    12181 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
    12241 gaactatatc cggatccact agttctagag cggccgccac cgcggtggag ctccagcttt
    12301 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
    12361 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    12421 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    12481 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    12541 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    12601 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    12661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    12721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    12781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    12841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    12901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    12961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    13021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    13081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    13141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    13201 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    13261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    13321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    13381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    13441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    13501 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    13561 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    13621 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    13681 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    13741 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    13801 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    13861 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    13921 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    13981 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    14041 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    14101 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    14161 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    14221 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    14281 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    14341 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    14401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    14461 ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
