LOCUS       sequence-81386-e        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single component LSL vector. Contains 2x35S:Zif268:FokI outside of 
            the replicon borders. Within the replicon is the us:NPTII template. 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pZLSLD.R
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(830..850)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(884..908)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(969..1015)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(1004..1024)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tccttctcac ttcctcaagt gtcaatgtac cagctttaat catttctcca ccaatcaaaa
       61 gctcttctac actaagaaca gctccattac aattagtgat atgattcaat cttgtaagct
      121 gagctttgta gtttcctttg aaatgaccac tcacaaacaa gaacttaaat tctgttactg
      181 aagatggata gactttccac cattcattag ggttgatatg cttgtttctt gtttgattct
      241 cttcgacata tctttgcatt tcatctgctt gaccaattgg aagattataa cctcctgaat
      301 atgccttagt atcaacgatc acaccgtaat caataggaga tccgacagta taaattgctc
      361 cgtctggttt ccttgatcca cccaaatgtt taccacgata accataaacc ttcataaaga
      421 actccatcac cttcatttca aggattctat cctgagttga atttcttgcg atttcaatca
      481 attcaatata ttcatgtggc acatatttca atttatgtct aagttcagat ttcttctctt
      541 ccaattcaga tttcactagt tccttctgac gaagatggat ctttgtatga cgcttccttt
      601 catcacttct tgcgaactta cgaccacaga tgtcacatgc gaatggtttc tcaccagtat
      661 gggttctgat atgtgtagtc aaatgatcgc tacgactaaa gttcctcata cagattctac
      721 actggaaagg cttctgtcct gtgtggattc taatatgcct agtgagttcg tctgacctac
      781 taaaacgtct atcacaagac tcaactggac aagcgtaagg caattcaaga acctttctct
      841 ttttctttgg aggggaggaa gccatggtga agggcgaatt cggagcctgc ttttttgtac
      901 aaacttgttt gatagcttgg cgcgcctcga gggggggccc ggtacccggg gatcctctag
      961 agtcgaggtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
     1021 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
     1081 tg
//
