LOCUS       sequence-81389-e       14517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TagRFP-T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14517)
  AUTHORS   Nakashiba T, Cushman JD, Pelkey KA, Renaudineau S, Buhl DL, McHugh 
            TJ, Rodriguez Barrera V, Chittajallu R, Iwamoto KS, McBain CJ, 
            Fanselow MS, Tonegawa S
  TITLE     Young dentate granule cells mediate pattern separation, whereas old 
            granule cells facilitate pattern completion.
  JOURNAL   Cell. 2012 Mar 30;149(1):188-201. doi: 10.1016/j.cell.2012.01.046. 
            Epub 2012 Feb 23.
  PUBMED    22365813
REFERENCE   2  (bases 1 to 14517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.01.046"; journalName: "Cell"; date: 
            "2012-03-30- 30"; volume: "149"; issue: "1"; pages: "188-201"
COMMENT     Sequence Label: pRVdG-4RFP
FEATURES             Location/Qualifiers
     source          1..14517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4695..5426
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             7178..7189
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12189..12207)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12203..12250
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12273..12289)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12273..12289)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12326..12346)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12326..12344)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12365..12381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12365..12381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12378..12400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12389..12405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12413..12419,12420..12437,12438..12443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12458..12479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12596..12613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12767..13355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12847..12866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13526..14317,14318..14386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14149..14168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14387..14491)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga
     4741 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
     4801 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
     4861 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaacc
     4921 acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga
     4981 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
     5041 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg
     5101 tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac cccgctgacg
     5161 gcggcctgga aggcagaacc gacatggccc tgaagctcgt gggcgggggc cacctgatct
     5221 gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg
     5281 tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
     5341 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac
     5401 ttaatggcat ggacgagctg tacaagtaag ctagcttata aagtgctggg tcatctaagc
     5461 ttttcagtcg agaaaaaaac attagatcag aagaacaact ggcaacactt ctcaacctga
     5521 gacttacttc aagatgctcg atcctggaga ggtctatgat gaccctattg acccaatcga
     5581 gttagaggct gaacccagag gaacccccat tgtccccaac atcttgagga actctgacta
     5641 caatctcaac tctcctttga tagaagatcc tgctagacta atgttagaat ggttaaaaac
     5701 agggaataga ccttatcgga tgactctaac agacaattgc tccaggtctt tcagagtttt
     5761 gaaagattat ttcaagaagg tagatttggg ttctctcaag gtgggcggaa tggctgcaca
     5821 gtcaatgatt tctctctggt tatatggtgc ccactctgaa tccaacagga gccggagatg
     5881 tataacagac ttggcccatt tctattccaa gtcgtccccc atagagaagc tgttgaatct
     5941 cacgctagga aatagagggc tgagaatccc cccagaggga gtgttaagtt gccttgagag
     6001 ggttgattat gataatgcat ttggaaggta tcttgccaac acgtattcct cttacttgtt
     6061 cttccatgta atcaccttat acatgaacgc cctagactgg gatgaagaaa agaccatcct
     6121 agcattatgg aaagatttaa cctcagtgga catcgggaag gacttggtaa agttcaaaga
     6181 ccaaatatgg ggactgctga tcgtgacaaa ggactttgtt tactcccaaa gttccaattg
     6241 tctttttgac agaaactaca cacttatgct aaaagatctt ttcttgtctc gcttcaactc
     6301 cttaatggtc ttgctctctc ccccagagcc ccgatactca gatgacttga tatctcaact
     6361 atgccagctg tacattgctg gggatcaagt cttgtctatg tgtggaaact ccggctatga
     6421 agtcatcaaa atattggagc catatgtcgt gaatagttta gtccagagag cagaaaagtt
     6481 taggcctctc attcattcct tgggagactt tcctgtattt ataaaagaca aggtaagtca
     6541 acttgaagag acgttcggtc cctgtgcaag aaggttcttt agggctctgg atcaattcga
     6601 caacatacat gacttggttt ttgtgtttgg ctgttacagg cattgggggc acccatatat
     6661 agattatcga aagggtctgt caaaactata tgatcaggtt caccttaaaa aaatgataga
     6721 taagtcctac caggagtgct tagcaagcga cctagccagg aggatcctta gatggggttt
     6781 tgataagtac tccaagtggt atctggattc aagattccta gcccgagacc accccttgac
     6841 tccttatatc aaaacccaaa catggccacc caaacatatt gtagacttgg tgggggatac
     6901 atggcacaag ctcccgatca cgcagatctt tgagattcct gaatcaatgg atccgtcaga
     6961 aatattggat gacaaatcac attctttcac cagaacgaga ctagcttctt ggctgtcaga
     7021 aaaccgaggg gggcctgttc ctagcgaaaa agttattatc acggccctgt ctaagccgcc
     7081 tgtcaatccc cgagagtttc tgaggtctat agacctcgga ggattgccag atgaagactt
     7141 gataattggc ctcaagccaa aggaacggga attgaagatt gaaggtcgat tctttgctct
     7201 aatgtcatgg aatctaagat tgtattttgt catcactgaa aaactcttgg ccaactacat
     7261 cttgccactt tttgacgcgc tgactatgac agacaacctg aacaaggtgt ttaaaaagct
     7321 gatcgacagg gtcaccgggc aagggctttt ggactattca agggtcacat atgcatttca
     7381 cctggactat gaaaagtgga acaaccatca aagattagag tcaacagagg atgtattttc
     7441 tgtcctagat caagtgtttg gattgaagag agtgttttct agaacacacg agttttttca
     7501 aaaggcctgg atctattatt cagacagatc agacctcatc gggttacggg aggatcaaat
     7561 atactgctta gatgcgtcca acggcccaac ctgttggaat ggccaggatg gcgggctaga
     7621 aggcttacgg cagaagggct ggagtctagt cagcttattg atgatagata gagaatctca
     7681 aatcaggaac acaagaacca aaatactagc tcaaggagac aaccaggttt tatgtccgac
     7741 atacatgttg tcgccagggc tatctcaaga ggggctcctc tatgaattgg agagaatatc
     7801 aaggaatgca ctttcgatat acagagccgt cgaggaaggg gcatctaagc tagggctgat
     7861 catcaagaaa gaagagacca tgtgtagtta tgacttcctc atctatggaa aaaccccttt
     7921 gtttagaggt aacatattgg tgcctgagtc caaaagatgg gccagagtct cttgcgtctc
     7981 taatgaccaa atagtcaacc tcgccaatat aatgtcgaca gtgtccacca atgcgctaac
     8041 agtggcacaa cactctcaat ctttgatcaa accgatgagg gattttctgc tcatgtcagt
     8101 acaggcagtc tttcactacc tgctatttag cccaatctta aagggaagag tttacaagat
     8161 tctgagcgct gaaggggaga gctttctcct agccatgtca aggataatct atctagatcc
     8221 ttctttggga gggatatctg gaatgtccct cggaagattc catatacgac agttctcaga
     8281 ccctgtctct gaagggttat ccttctggag agagatctgg ttaagctccc aagagtcctg
     8341 gattcacgcg ttgtgtcaag aggctggaaa cccagatctt ggagagagaa cactcgagag
     8401 cttcactcgc cttctagaag atccgaccac cttaaatatc agaggagggg ccagtcctac
     8461 cattctactc aaggatgcaa tcagaaaggc tttatatgac gaggtggaca aggtggaaaa
     8521 ttcagagttt cgagaggcaa tcctgttgtc caagacccat agagataatt ttatactctt
     8581 cttaatatct gttgagcctc tgtttcctcg atttctcagt gagctattca gttcgtcttt
     8641 tttgggaatc cccgagtcaa tcattggatt gatacaaaac tcccgaacga taagaaggca
     8701 gtttagaaag agtctctcaa aaactttaga agaatccttc tacaactcag agatccacgg
     8761 gattagtcgg atgacccaga cacctcagag ggttgggggg gtgtggcctt gctcttcaga
     8821 gagggcagat ctacttaggg agatctcttg gggaagaaaa gtggtaggca cgacagttcc
     8881 tcacccttct gagatgttgg gattacttcc caagtcctct atttcttgca cttgtggagc
     8941 aacaggagga ggcaatccta gagtttctgt atcagtactc ccgtcctttg atcagtcatt
     9001 tttttcacga ggccccctaa agggatactt gggctcgtcc acctctatgt cgacccagct
     9061 attccatgca tgggaaaaag tcactaatgt tcatgtggtg aagagagctc tatcgttaaa
     9121 agaatctata aactggttca ttactagaga ttccaacttg gctcaagctc taattaggaa
     9181 cattatgtct ctgacaggcc ctgatttccc tctagaggag gcccctgtct tcaaaaggac
     9241 ggggtcagcc ttgcataggt tcaagtctgc cagatacagc gaaggagggt attcttctgt
     9301 ctgcccgaac ctcctctctc atatttctgt tagtacagac accatgtctg atttgaccca
     9361 agacgggaag aactacgatt tcatgttcca gccattgatg ctttatgcac agacatggac
     9421 atcagagctg gtacagagag acacaaggct aagagactct acgtttcatt ggcacctccg
     9481 atgcaacagg tgtgtgagac ccattgacga cgtgaccctg gagacctctc agatcttcga
     9541 gtttccggat gtgtcgaaaa gaatatccag aatggtttct ggggctgtgc ctcacttcca
     9601 gaggcttccc gatatccgtc tgagaccagg agattttgaa tctctaagcg gtagagaaaa
     9661 gtctcaccat atcggatcag ctcaggggct cttatactca atcttagtgg caattcacga
     9721 ctcaggatac aatgatggaa ccatcttccc tgtcaacata tacggcaagg tttcccctag
     9781 agactatttg agagggctcg caaggggagt attgatagga tcctcgattt gcttcttgac
     9841 aagaatgaca aatatcaata ttaatagacc tcttgaattg gtctcagggg taatctcata
     9901 tattctcctg aggctagata accatccctc cttgtacata atgctcagag aaccgtctct
     9961 tagaggagag atattttcta tccctcagaa aatccccgcc gcttatccaa ccactatgaa
    10021 agaaggcaac agatcaatct tgtgttatct ccaacatgtg ctacgctatg agcgagagat
    10081 aatcacggcg tctccagaga atgactggct atggatcttt tcagacttta gaagtgccaa
    10141 aatgacgtac ctatccctca ttacttacca gtctcatctt ctactccaga gggttgagag
    10201 aaacctatct aagagtatga gagataacct gcgacaattg agttctttga tgaggcaggt
    10261 gctgggcggg cacggagaag ataccttaga gtcagacgac aacattcaac gactgctaaa
    10321 agactcttta cgaaggacaa gatgggtgga tcaagaggtg cgccatgcag ctagaaccat
    10381 gactggagat tacagcccca acaagaaggt gtcccgtaag gtaggatgtt cagaatgggt
    10441 ctgctctgct caacaggttg cagtctctac ctcagcaaac ccggcccctg tctcggagct
    10501 tgacataagg gccctctcta agaggttcca gaaccctttg atctcgggct tgagagtggt
    10561 tcagtgggca accggtgctc attataagct taagcctatt ctagatgatc tcaatgtttt
    10621 cccatctctc tgccttgtag ttggggacgg gtcagggggg atatcaaggg cagtcctcaa
    10681 catgtttcca gatgccaagc ttgtgttcaa cagtctttta gaggtgaatg acctgatggc
    10741 ttccggaaca catccactgc ctccttcagc aatcatgagg ggaggaaatg atatcgtctc
    10801 cagagtgata gatcttgact caatctggga aaaaccgtcc gacttgagaa acttggcaac
    10861 ctggaaatac ttccagtcag tccaaaagca ggtcaacatg tcctatgacc tcattatttg
    10921 cgatgcagaa gttactgaca ttgcatctat caaccggatc accctgttaa tgtccgattt
    10981 tgcattgtct atagatggac cactctattt ggtcttcaaa acttatggga ctatgctagt
    11041 aaatccaaac tacaaggcta ttcaacacct gtcaagagcg ttcccctcgg tcacagggtt
    11101 tatcacccaa gtaacttcgt ctttttcatc tgagctctac ctccgattct ccaaacgagg
    11161 gaagtttttc agagatgctg agtacttgac ctcttccacc cttcgagaaa tgagccttgt
    11221 gttattcaat tgtagcagcc ccaagagtga gatgcagaga gctcgttcct tgaactatca
    11281 ggatcttgtg agaggatttc ctgaagaaat catatcaaat ccttacaatg agatgatcat
    11341 aactctgatt gacagtgatg tagaatcttt tctagtccac aagatggttg atgatcttga
    11401 gttacagagg ggaactctgt ctaaagtggc tatcattata gccatcatga tagttttctc
    11461 caacagagtc ttcaacgttt ccaaacccct aactgacccc tcgttctatc caccgtctga
    11521 tcccaaaatc ctgaggcact tcaacatatg ttgcagtact atgatgtatc tatctactgc
    11581 tttaggtgac gtccctagct tcgcaagact tcacgacctg tataacagac ctataactta
    11641 ttacttcaga aagcaagtca ttcgagggaa cgtttatcta tcttggagtt ggtccaacga
    11701 cacctcagtg ttcaaaaggg tagcctgtaa ttctagcctg agtctgtcat ctcactggat
    11761 caggttgatt tacaagatag tgaagactac cagactcgtt ggcagcatca aggatctatc
    11821 cagagaagtg gaaagacacc ttcataggta caacaggtgg atcaccctag aggatatcag
    11881 atctagatca tccctactag actacagttg cctgtgaacc ggatactcct ggaagcctgc
    11941 ccatgctaag actcttgtgt gatgtatctt gaaaaaaaca agatcctaaa tctgaacctt
    12001 tggttgtttg attgtttttc tcatttttgt tgtttatttg ttaagcgtgg gtcggcatgg
    12061 catctccacc tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga
    12121 tggctaaggg aggggcgggg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
    12181 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
    12241 gggttttttg ctgaaaggag gaactatatc cggatccact agttctagag cggccgccac
    12301 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
    12361 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    12421 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    12481 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    12541 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    12601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    12661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    12721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    12781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    12841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    12901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    12961 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    13021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    13081 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    13141 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    13201 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    13261 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    13321 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    13381 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    13441 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    13501 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    13561 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    13621 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    13681 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    13741 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    13801 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    13861 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    13921 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    13981 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    14041 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    14101 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    14161 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    14221 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    14281 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    14341 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    14401 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    14461 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
