LOCUS       sequence-81390-e       14487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14487)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4BFP2
FEATURES             Location/Qualifiers
     source          1..14487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4695..5396
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             7148..7159
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12159..12177)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12173..12220
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12243..12259)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12243..12259)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12296..12316)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12296..12314)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12335..12351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12335..12351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12348..12370)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12359..12375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12383..12389,12390..12407,12408..12413))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12428..12449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12566..12583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12737..13325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12817..12836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13496..14287,14288..14356))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14119..14138
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14357..14461)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     4741 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     4801 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     4861 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     4921 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     4981 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     5041 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     5101 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     5161 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga
     5221 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     5281 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     5341 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aattaatgag
     5401 ctagcttata aagtgctggg tcatctaagc ttttcagtcg agaaaaaaac attagatcag
     5461 aagaacaact ggcaacactt ctcaacctga gacttacttc aagatgctcg atcctggaga
     5521 ggtctatgat gaccctattg acccaatcga gttagaggct gaacccagag gaacccccat
     5581 tgtccccaac atcttgagga actctgacta caatctcaac tctcctttga tagaagatcc
     5641 tgctagacta atgttagaat ggttaaaaac agggaataga ccttatcgga tgactctaac
     5701 agacaattgc tccaggtctt tcagagtttt gaaagattat ttcaagaagg tagatttggg
     5761 ttctctcaag gtgggcggaa tggctgcaca gtcaatgatt tctctctggt tatatggtgc
     5821 ccactctgaa tccaacagga gccggagatg tataacagac ttggcccatt tctattccaa
     5881 gtcgtccccc atagagaagc tgttgaatct cacgctagga aatagagggc tgagaatccc
     5941 cccagaggga gtgttaagtt gccttgagag ggttgattat gataatgcat ttggaaggta
     6001 tcttgccaac acgtattcct cttacttgtt cttccatgta atcaccttat acatgaacgc
     6061 cctagactgg gatgaagaaa agaccatcct agcattatgg aaagatttaa cctcagtgga
     6121 catcgggaag gacttggtaa agttcaaaga ccaaatatgg ggactgctga tcgtgacaaa
     6181 ggactttgtt tactcccaaa gttccaattg tctttttgac agaaactaca cacttatgct
     6241 aaaagatctt ttcttgtctc gcttcaactc cttaatggtc ttgctctctc ccccagagcc
     6301 ccgatactca gatgacttga tatctcaact atgccagctg tacattgctg gggatcaagt
     6361 cttgtctatg tgtggaaact ccggctatga agtcatcaaa atattggagc catatgtcgt
     6421 gaatagttta gtccagagag cagaaaagtt taggcctctc attcattcct tgggagactt
     6481 tcctgtattt ataaaagaca aggtaagtca acttgaagag acgttcggtc cctgtgcaag
     6541 aaggttcttt agggctctgg atcaattcga caacatacat gacttggttt ttgtgtttgg
     6601 ctgttacagg cattgggggc acccatatat agattatcga aagggtctgt caaaactata
     6661 tgatcaggtt caccttaaaa aaatgataga taagtcctac caggagtgct tagcaagcga
     6721 cctagccagg aggatcctta gatggggttt tgataagtac tccaagtggt atctggattc
     6781 aagattccta gcccgagacc accccttgac tccttatatc aaaacccaaa catggccacc
     6841 caaacatatt gtagacttgg tgggggatac atggcacaag ctcccgatca cgcagatctt
     6901 tgagattcct gaatcaatgg atccgtcaga aatattggat gacaaatcac attctttcac
     6961 cagaacgaga ctagcttctt ggctgtcaga aaaccgaggg gggcctgttc ctagcgaaaa
     7021 agttattatc acggccctgt ctaagccgcc tgtcaatccc cgagagtttc tgaggtctat
     7081 agacctcgga ggattgccag atgaagactt gataattggc ctcaagccaa aggaacggga
     7141 attgaagatt gaaggtcgat tctttgctct aatgtcatgg aatctaagat tgtattttgt
     7201 catcactgaa aaactcttgg ccaactacat cttgccactt tttgacgcgc tgactatgac
     7261 agacaacctg aacaaggtgt ttaaaaagct gatcgacagg gtcaccgggc aagggctttt
     7321 ggactattca agggtcacat atgcatttca cctggactat gaaaagtgga acaaccatca
     7381 aagattagag tcaacagagg atgtattttc tgtcctagat caagtgtttg gattgaagag
     7441 agtgttttct agaacacacg agttttttca aaaggcctgg atctattatt cagacagatc
     7501 agacctcatc gggttacggg aggatcaaat atactgctta gatgcgtcca acggcccaac
     7561 ctgttggaat ggccaggatg gcgggctaga aggcttacgg cagaagggct ggagtctagt
     7621 cagcttattg atgatagata gagaatctca aatcaggaac acaagaacca aaatactagc
     7681 tcaaggagac aaccaggttt tatgtccgac atacatgttg tcgccagggc tatctcaaga
     7741 ggggctcctc tatgaattgg agagaatatc aaggaatgca ctttcgatat acagagccgt
     7801 cgaggaaggg gcatctaagc tagggctgat catcaagaaa gaagagacca tgtgtagtta
     7861 tgacttcctc atctatggaa aaaccccttt gtttagaggt aacatattgg tgcctgagtc
     7921 caaaagatgg gccagagtct cttgcgtctc taatgaccaa atagtcaacc tcgccaatat
     7981 aatgtcgaca gtgtccacca atgcgctaac agtggcacaa cactctcaat ctttgatcaa
     8041 accgatgagg gattttctgc tcatgtcagt acaggcagtc tttcactacc tgctatttag
     8101 cccaatctta aagggaagag tttacaagat tctgagcgct gaaggggaga gctttctcct
     8161 agccatgtca aggataatct atctagatcc ttctttggga gggatatctg gaatgtccct
     8221 cggaagattc catatacgac agttctcaga ccctgtctct gaagggttat ccttctggag
     8281 agagatctgg ttaagctccc aagagtcctg gattcacgcg ttgtgtcaag aggctggaaa
     8341 cccagatctt ggagagagaa cactcgagag cttcactcgc cttctagaag atccgaccac
     8401 cttaaatatc agaggagggg ccagtcctac cattctactc aaggatgcaa tcagaaaggc
     8461 tttatatgac gaggtggaca aggtggaaaa ttcagagttt cgagaggcaa tcctgttgtc
     8521 caagacccat agagataatt ttatactctt cttaatatct gttgagcctc tgtttcctcg
     8581 atttctcagt gagctattca gttcgtcttt tttgggaatc cccgagtcaa tcattggatt
     8641 gatacaaaac tcccgaacga taagaaggca gtttagaaag agtctctcaa aaactttaga
     8701 agaatccttc tacaactcag agatccacgg gattagtcgg atgacccaga cacctcagag
     8761 ggttgggggg gtgtggcctt gctcttcaga gagggcagat ctacttaggg agatctcttg
     8821 gggaagaaaa gtggtaggca cgacagttcc tcacccttct gagatgttgg gattacttcc
     8881 caagtcctct atttcttgca cttgtggagc aacaggagga ggcaatccta gagtttctgt
     8941 atcagtactc ccgtcctttg atcagtcatt tttttcacga ggccccctaa agggatactt
     9001 gggctcgtcc acctctatgt cgacccagct attccatgca tgggaaaaag tcactaatgt
     9061 tcatgtggtg aagagagctc tatcgttaaa agaatctata aactggttca ttactagaga
     9121 ttccaacttg gctcaagctc taattaggaa cattatgtct ctgacaggcc ctgatttccc
     9181 tctagaggag gcccctgtct tcaaaaggac ggggtcagcc ttgcataggt tcaagtctgc
     9241 cagatacagc gaaggagggt attcttctgt ctgcccgaac ctcctctctc atatttctgt
     9301 tagtacagac accatgtctg atttgaccca agacgggaag aactacgatt tcatgttcca
     9361 gccattgatg ctttatgcac agacatggac atcagagctg gtacagagag acacaaggct
     9421 aagagactct acgtttcatt ggcacctccg atgcaacagg tgtgtgagac ccattgacga
     9481 cgtgaccctg gagacctctc agatcttcga gtttccggat gtgtcgaaaa gaatatccag
     9541 aatggtttct ggggctgtgc ctcacttcca gaggcttccc gatatccgtc tgagaccagg
     9601 agattttgaa tctctaagcg gtagagaaaa gtctcaccat atcggatcag ctcaggggct
     9661 cttatactca atcttagtgg caattcacga ctcaggatac aatgatggaa ccatcttccc
     9721 tgtcaacata tacggcaagg tttcccctag agactatttg agagggctcg caaggggagt
     9781 attgatagga tcctcgattt gcttcttgac aagaatgaca aatatcaata ttaatagacc
     9841 tcttgaattg gtctcagggg taatctcata tattctcctg aggctagata accatccctc
     9901 cttgtacata atgctcagag aaccgtctct tagaggagag atattttcta tccctcagaa
     9961 aatccccgcc gcttatccaa ccactatgaa agaaggcaac agatcaatct tgtgttatct
    10021 ccaacatgtg ctacgctatg agcgagagat aatcacggcg tctccagaga atgactggct
    10081 atggatcttt tcagacttta gaagtgccaa aatgacgtac ctatccctca ttacttacca
    10141 gtctcatctt ctactccaga gggttgagag aaacctatct aagagtatga gagataacct
    10201 gcgacaattg agttctttga tgaggcaggt gctgggcggg cacggagaag ataccttaga
    10261 gtcagacgac aacattcaac gactgctaaa agactcttta cgaaggacaa gatgggtgga
    10321 tcaagaggtg cgccatgcag ctagaaccat gactggagat tacagcccca acaagaaggt
    10381 gtcccgtaag gtaggatgtt cagaatgggt ctgctctgct caacaggttg cagtctctac
    10441 ctcagcaaac ccggcccctg tctcggagct tgacataagg gccctctcta agaggttcca
    10501 gaaccctttg atctcgggct tgagagtggt tcagtgggca accggtgctc attataagct
    10561 taagcctatt ctagatgatc tcaatgtttt cccatctctc tgccttgtag ttggggacgg
    10621 gtcagggggg atatcaaggg cagtcctcaa catgtttcca gatgccaagc ttgtgttcaa
    10681 cagtctttta gaggtgaatg acctgatggc ttccggaaca catccactgc ctccttcagc
    10741 aatcatgagg ggaggaaatg atatcgtctc cagagtgata gatcttgact caatctggga
    10801 aaaaccgtcc gacttgagaa acttggcaac ctggaaatac ttccagtcag tccaaaagca
    10861 ggtcaacatg tcctatgacc tcattatttg cgatgcagaa gttactgaca ttgcatctat
    10921 caaccggatc accctgttaa tgtccgattt tgcattgtct atagatggac cactctattt
    10981 ggtcttcaaa acttatggga ctatgctagt aaatccaaac tacaaggcta ttcaacacct
    11041 gtcaagagcg ttcccctcgg tcacagggtt tatcacccaa gtaacttcgt ctttttcatc
    11101 tgagctctac ctccgattct ccaaacgagg gaagtttttc agagatgctg agtacttgac
    11161 ctcttccacc cttcgagaaa tgagccttgt gttattcaat tgtagcagcc ccaagagtga
    11221 gatgcagaga gctcgttcct tgaactatca ggatcttgtg agaggatttc ctgaagaaat
    11281 catatcaaat ccttacaatg agatgatcat aactctgatt gacagtgatg tagaatcttt
    11341 tctagtccac aagatggttg atgatcttga gttacagagg ggaactctgt ctaaagtggc
    11401 tatcattata gccatcatga tagttttctc caacagagtc ttcaacgttt ccaaacccct
    11461 aactgacccc tcgttctatc caccgtctga tcccaaaatc ctgaggcact tcaacatatg
    11521 ttgcagtact atgatgtatc tatctactgc tttaggtgac gtccctagct tcgcaagact
    11581 tcacgacctg tataacagac ctataactta ttacttcaga aagcaagtca ttcgagggaa
    11641 cgtttatcta tcttggagtt ggtccaacga cacctcagtg ttcaaaaggg tagcctgtaa
    11701 ttctagcctg agtctgtcat ctcactggat caggttgatt tacaagatag tgaagactac
    11761 cagactcgtt ggcagcatca aggatctatc cagagaagtg gaaagacacc ttcataggta
    11821 caacaggtgg atcaccctag aggatatcag atctagatca tccctactag actacagttg
    11881 cctgtgaacc ggatactcct ggaagcctgc ccatgctaag actcttgtgt gatgtatctt
    11941 gaaaaaaaca agatcctaaa tctgaacctt tggttgtttg attgtttttc tcatttttgt
    12001 tgtttatttg ttaagcgtgg gtcggcatgg catctccacc tcctcgcggt ccgacctggg
    12061 catccgaagg aggacgcacg tccactcgga tggctaaggg aggggcgggg atccggctgc
    12121 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
    12181 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
    12241 cggatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
    12301 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    12361 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    12421 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    12481 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    12541 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    12601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    12661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    12721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    12781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    12841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    12901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    12961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    13021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    13081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    13141 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    13201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    13261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    13321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    13381 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    13441 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    13501 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    13561 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    13621 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    13681 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    13741 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    13801 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    13861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    13921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    13981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    14041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    14101 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    14161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    14221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    14281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    14341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    14401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    14461 gcacatttcc ccgaaaagtg ccacctg
//
