LOCUS       sequence-81393-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ 
            indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Saito K, Hatsugai N, Horikawa K, Kobayashi K, Matsu-Ura T, Mikoshiba
            K, Nagai T
  TITLE     Auto-luminescent genetically-encoded ratiometric indicator for 
            real-time Ca2+ imaging at the single cell level.
  JOURNAL   PLoS One. 2010 Apr 1;5(4):e9935. doi: 10.1371/journal.pone.0009935.
  PUBMED    20376337
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-04-1"; volume: "5(4):e9935. doi"; pages: " 
            10.1371/journal.pone.0009935"
COMMENT     Sequence Label: BRAC/pcDNA3
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             459..536
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 agcgcatgca tgaccaactg acagaagagc agattgcaga gttcaaagaa gccttctcat
       61 tattcgacaa ggatggggac ggcaccatca ccacaaagga acttggcacc gttatgaggt
      121 cgcttggaca aaacccaacg gaagcagaat tgcaggatat gatcaatgaa gtcgatgctg
      181 atggcaatgg aacgatttac tttcctgaat ttcttactat gatggctaga aaaatgaagg
      241 acacagacag cgaagaggaa atccgagaag cattccgtgt ttttgacaag gatgggaacg
      301 gctacatcag cgctgctcag ttacgtcacg tcatgacaaa cctcggggag aagttaacag
      361 atgaagaagt tgatgaaatg ataagggaag cagatatcga tggtgatggc caagtaaact
      421 atgaagagtt tgtacaaatg atgacagcaa agggggggaa gaggcgctgg aagaaaaact
      481 tcattgccgt cagcgctgcc aaccggttca agaagatctc cagctccggg gcactggagc
      541 tcatgaccag caaggtgtac gaccccgagc agaggaagag gatgatcacc ggcccccagt
      601 ggtgggccag gtgcaagcag atgaacgtgc tggacagctt catcaactac tacgacagcg
      661 agaagcacgc cgagaacgcc gtgatcttcc tgcacggcaa cgccactagc agctacctgt
      721 ggaggcacgt ggtgccccac atcgagcccg tggccaggtg catcatcccc gatctgatcg
      781 gcatgggcaa gagcggcaag agcggcaacg gcagctacag gctgctggac cactacaagt
      841 acctgaccgc ctggttcgag ctcctgaacc tgcccaagaa gatcatctt
//
