LOCUS       sequence-81408-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV plasmid with FEV (Ple67) promoter driving expression of iCre. 
            Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1991
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(17..23,24..41,42..47))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    62..83
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(200..217)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(451..470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgtcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
       61 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      121 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      181 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      241 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      301 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      361 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      421 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      481 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      541 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      601 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      661 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      721 ca
//
