LOCUS       sequence-81417-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast plasmid for integration of target sequence at HO locus, Kan 
            selection, pGAL inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: HO-pGAL-poly-KanMX4-HO
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(31..47)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(31..47)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(62..503)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(68..91)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    386..503
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        576..919
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagaatc cggggttttt
       61 tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac ttttattaca
      121 tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag tggttatgca
      181 gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg attaattacc
      241 ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt
      301 tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag
      361 ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca
      421 ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg agggctgtca
      481 cccgctcggc ggcttctaat ccgtactaga ggggttaatt aaggcgcgcc agatctgttt
      541 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
      601 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
      661 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
      721 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
      781 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
      841 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
      901 catccgaaca taaacaacca tggg
//
