LOCUS       sequence-81420-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of a 'caged' Ca2+ indicator for fluorescent 
            imaging at specific locations and time points.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Matsuda T, Horikawa K, Saito K, Nagai T
  TITLE     Highlighted Ca(2)(+) imaging with a genetically encoded 'caged' 
            indicator.
  JOURNAL   Sci Rep. 2013;3:1398. doi: 10.1038/srep01398.
  PUBMED    23474844
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:1398. doi"; pages: " 10.1038/srep01398"
COMMENT     Sequence Label: PA-TNXL/pcDNA3
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..788)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     247..266
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     506..527
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(630..651)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctagatgcat gctcgagcgg ccgccagtgt gatggatatc tgcagaattc ttactcgatg
       61 ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc ggtgatatag
      121 acgttgtggc tgttccagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      181 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      241 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
      301 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag
      361 cactgcaggc cgtagcccag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      421 gtggtgcaga tcagcttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
      481 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      541 ccggtgaaca gctcctcgcc cttgctcacc atgccaccgc tgccaccctt gtacagctcg
      601 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      661 ttctcgttgg ggtctttgct cagggcggac tggtagctca ggtagtggtt gtcgggcagc
      721 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgccg
      781 ccgtccatga gctcctgcac accctccatc atcttcagga actcatcgaa gtcaatgcgg
      841 ccgttattgt ccttgtctga atcc
//
