LOCUS       sequence-81421-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pMDC32 with T30 TALEN pair followed by the us:NPTII donor molecule.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: p35ST
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        406..750
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     694..714
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    810..834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             856..921
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             928..948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc
       61 agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg
      121 attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc
      181 ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag
      241 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
      301 cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca cacttgtcta
      361 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
      421 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
      481 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
      541 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
      601 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
      661 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
      721 ataaggaagt tcatttcatt tggagaggac ctcgactcta gaggatcccc gggtaccggg
      781 ccccccctcg aggcgcgcca agctatcaaa caagtttgta caaaaaagca ggctccgaat
      841 tcgcccttca ccatggatta taaggatcac gatggcgact acaaggacca cgatattgac
      901 tacaaagacg atgacgataa aatggctcct aagaaaaagc gcaaagtcgg tatccatggc
      961 gttccctcta gaatggtgga tctacgcacg ctcggctac
//
