LOCUS       sequence-81422-e        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pMDC32 with Zif268:FokI followed by us:NPTII repair template 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: p35SZ.D
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(831..851)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(885..909)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(970..1016)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(1005..1025)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ttccttctca cttcctcaag tgtcaatgta ccagctttaa tcatttctcc accaatcaaa
       61 agctcttcta cactaagaac agctccatta caattagtga tatgattcaa tcttgtaagc
      121 tgagctttgt agtttccttt gaaatgacca ctcacaaaca agaacttaaa ttctgttact
      181 gaagatggat agactttcca ccattcatta gggttgatat gcttgtttct tgtttgattc
      241 tcttcgacat atctttgcat ttcatctgct tgaccaattg gaagattata acctcctgaa
      301 tatgccttag tatcaacgat cacaccgtaa tcaataggag atccgacagt ataaattgct
      361 ccgtctggtt tccttgatcc acccaaatgt ttaccacgat aaccataaac cttcataaag
      421 aactccatca ccttcatttc aaggattcta tcctgagttg aatttcttgc gatttcaatc
      481 aattcaatat attcatgtgg cacatatttc aatttatgtc taagttcaga tttcttctct
      541 tccaattcag atttcactag ttccttctga cgaagatgga tctttgtatg acgcttcctt
      601 tcatcacttc ttgcgaactt acgaccacag atgtcacatg cgaatggttt ctcaccagta
      661 tgggttctga tatgtgtagt caaatgatcg ctacgactaa agttcctcat acagattcta
      721 cactggaaag gcttctgtcc tgtgtggatt ctaatatgcc tagtgagttc gtctgaccta
      781 ctaaaacgtc tatcacaaga ctcaactgga caagcgtaag gcaattcaag aacctttctc
      841 tttttctttg gaggggagga agccatggtg aagggcgaat tcggagcctg cttttttgta
      901 caaacttgtt tgatagcttg gcgcgcctcg agggggggcc cggtacccgg ggatcctcta
      961 gagtcgaggt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     1021 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgc
//
