LOCUS       sequence-81423-e         249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single component LSL vector with GFP. Rep is expressed from the 
            complementary sense LIR promoter. GFP is between LIR and SIR and is 
            driven from a 2X35S promoter. Rep is within the replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLGFP.R
FEATURES             Location/Qualifiers
     source          1..249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..54
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
       61 gatagtttaa actatgaggg tcgtacgaat aattcgtatc caacggaaat acctgataca
      121 atatacgctc catcaaatac catcacatcg tatatgcttt tatagtgtga acacctttaa
      181 ccctagtggg cgggaaattt tctactttaa atctggaccg ctcgtgctaa agcactcgcg
      241 ataaggggg
//
