LOCUS       sequence-81424-e        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single component LSL vector with GFP. Rep is expressed from the 
            complementary sense LIR promoter. GFP is between LIR and SIR and is 
            driven from a 2X35S promoter. Rep is within the replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLGFP.R
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(48..393)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(85..105)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tccagtgaaa agttcttctc ctttactcac cattctagag tcccccgtgt tctctccaaa
       61 tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca
      121 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
      181 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
      241 gaggcatctt caacgatggc ctttccttta tcgcaatgat ggcatttgta ggagccacct
      301 tccttttcca ctatcttcac aataaagtga cagatagctg ggcaatggaa tccgaggagg
      361 tttccggata ttaccctttg ttgaaaagtc tcaattgccc tttggtcttc tgagactgta
      421 tctttgatat ttttggagta gacaagtgtg tcgtgctcca ccatgttgac gaagattttc
      481 ttcttgtcat tgagtcgtaa gagactctgt atgaactgtt cgccagtctt tacggcgagt
      541 tctgttaggt cctctatttg aatctttgac tccatggcct ttgattcagt gggaactacc
      601 tttttagaga ctccaatctc tattacttgc cttggtttgt gaagcaagcc ttgaatcgtc
      661 catactggaa tagtacttct gatcttgaga aatatatctt tctctgtgtt cttgatgcag
      721 ttagtcctga atcttttgac tgcatcttta accttcttgg gaaggtattt gatttcctgg
      781 agattattgc tcgggtagat cgtcttgatg agacctgctg cgtaagcctc tctaaccatc
      841 tgtgggttag cattctttct gaaattgaaa aggcaaatct ggggaccgtt taaactatca
      901 ggtgacgtac ccaaagctct ggaagttctc ggcgcgccga gggtcgtacg aataattcgt
      961 atccaacgga aatacctgat acaatatacg ctccatcaaa taccatcaca tcgtatatgc
     1021 ttttatagtg tgaacacctt taaccctagt gggcgggaaa
//
