LOCUS       sequence-81425-e        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single component LSL vector with GFP. Rep is expressed from the 
            complementary sense LIR promoter. GFP is between LIR and SIR and is 
            driven from a 2X35S promoter. Rep is within the replicon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLGFP.R
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      43..295
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 catggcatgg atgaactata caaataaagc tcgaatttcc ccgatcgttc aaacatttgg
       61 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
      121 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
      181 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
      241 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgtggg
      301 cccagctcca gacgtcgcgg tggtggctcg agcgcgtagt cctcggtaat atcgcagaac
      361 aaaagtacct gatatcgagt gtacttcaag tcagtgggaa atcaataaaa tgattatttt
      421 atgaatatat ttcattgtgc aagtagatag aaattacata tgttacataa cacacgaaat
      481 aaacaaaaaa agacaatcca aaaacaaaca ccccaaaaaa aataatcact ttagataaac
      541 tcgtatgagg agaggcacgt tcagtgactc gacgattccc gagcaaaaaa agtctccccg
      601 tcacacatgt agtgggtgac gcaattatct ttaaagtaat ccttctgttg acttgtcatt
      661 gataacatcc agtcttcgtc aggattgcaa agaattatag aagggatccc accttttatt
      721 ttcttctttt ttccatattt agggttgaca gtgaaatcag actggcaacc tattaattgc
      781 ttccacaatg ggacgaactt gaaggggatg tcgtcgatga tattataggt ggcgtgttca
      841 tcgtagttgg tgaaatcgat ggtaccgttc caatagttgt gtcgtccgag acttctagcc
      901 caggtggtct ttccggtacg agttggtccg cagatgtaga ggctggggtg tcggattcca
      961 ttccttccat tgtccttgtt aaatcggcca tccattcaag gtcagattga gcttgttggt
     1021 atgagacagg atgtatgtaa gtataagcgt ctatgcttac atggtataga tggg
//
