LOCUS       sequence-81428-e        9220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HPV35 L1 and L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
  TITLE     Impact of inhibitors and L2 antibodies upon the infectivity of 
            diverse alpha and beta human papillomavirus types.
  JOURNAL   PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
            eCollection 2014.
  PUBMED    24816794
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: " 
            10.1371/journal.pone.0097232. eCollection 2014"
COMMENT     Sequence Label: pVITRO-HPV35 L1L2
FEATURES             Location/Qualifiers
     source          1..9220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    3119..3240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3130..3149)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3184..3203
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3428..4016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3917..3936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4096..4399
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4514..5819
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4856..5787
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    7263..7707
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7740..7786
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7778..7796
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7806..8600
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7806..8600
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7860..7879)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8470..8489
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8647..9219
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtgat atcaaagatc cgccaccatg agcctgtggc ggagcaacga ggccaccgtg
     1621 tacctgcccc ccgtgtccgt gagcaaggtg gtgtccaccg acgagtacgt gacccggacc
     1681 aacatctact accacgccgg cagcagcaga ctgctggccg tgggccaccc ctactacgcc
     1741 atcaagaagc aggactctaa caagatcgca gtgcccaagg tgtccggcct gcagtaccgg
     1801 gtgttcagag tgaagctgcc cgaccccaac aagttcggct tccccgacac cagcttctac
     1861 gaccccgcca gccagagact ggtctgggcc tgcaccggcg tggaagtggg cagaggacag
     1921 ccactgggcg tgggcatctc tggccaccct ctgctgaaca agctggacga caccgagaac
     1981 agcaataagt acgtgggcaa ctccggcacc gacaaccggg agtgcatcag catggactac
     2041 aagcagaccc agctgtgcct gatcggctgc agacccccca tcggcgagca ctggggcaag
     2101 ggcaccccct gcaacgccaa ccaggtcaag gccggcgagt gcccccctct ggaactgctg
     2161 aacaccgtgc tgcaggacgg cgacatggtg gacaccggct tcggcgccat ggacttcacc
     2221 acactgcagg caaacaagtc cgacgtgccc ctggacatct gcagcagcat ctgcaagtac
     2281 cccgactacc tgaagatggt gtccgagccc tacggcgata tgctgttctt ctacctgcgg
     2341 cgggagcaga tgttcgtgcg gcacctgttc aacagagccg gcaccgtggg cgagacagtg
     2401 cccgccgacc tgtacatcaa gggcaccacc ggcacactgc ccagcaccag ctacttcccc
     2461 acccccagcg gcagcatggt caccagcgac gcccagatct tcaacaagcc ctactggctg
     2521 cagcgggccc agggccacaa caacggcatc tgctggtcca accagctgtt cgtgaccgtg
     2581 gtcgatacca ctcggtccac caacatgagc gtgtgcagcg ccgtgagcag cagcgacagc
     2641 acctacaaga acgacaactt caaagagtac ctgcggcacg gcgaggaata cgacctgcag
     2701 ttcatcttcc agctgtgcaa gatcaccctg accgccgacg tgatgaccta catccacagc
     2761 atgaacccca gcatcctgga agattggaac ttcggcctga ccccccctcc tagcggcacc
     2821 ctggaagata cctacagata cgtgaccagc caggccgtga cctgccagaa gcccagcgcc
     2881 cccaagccca aggacgaccc cctgaagaac tacaccttct gggaggtcga cctgaaagag
     2941 aagttcagcg ctgacctgga ccagttcccc ctgggccgga agttcctgct gcaagctggc
     3001 ctgaaggcca gacccaactt ccggctgggc aagagagccg cccctgcctc cacctccaag
     3061 aagtccagca ccaagcggcg gaaagtgaag tcctgatagt ctagctggcc agacatgata
     3121 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3181 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3241 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     3301 taaagcaagt aaaacctcta caaatgtggt atggaaatgt taattaacta gccatgacca
     3361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4081 taattaacct gcaggcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4141 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4201 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4261 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4321 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4381 agtcatcgct attaccatga tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     4441 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     4501 actagtggag ccgagagtaa ttcatacaaa aggagggatc gccttcgcaa ggggagagcc
     4561 cagggaccgt ccctaaattc tcacagaccc aaatccctgt agccgcccca cgacagcgcg
     4621 aggagcatgc gcccagggct gagcgcgggt agatcagagc acacaagctc acagtccccg
     4681 gcggtggggg gaggggcgcg ctgagcgggg gccagggagc tggcgcgggg caaactggga
     4741 aagtggtgtc gtgtgctggc tccgccctct tcccgagggt gggggagaac ggtatataag
     4801 tgcggtagtc gccttggacg ttctttttcg caacgggttt gccgtcagaa cgcaggtgag
     4861 tggcgggtgt ggcttccgcg ggccccggag ctggagccct gctctgagcg ggccgggctg
     4921 atatgcgagt gtcgtccgca gggtttagct gtgagcattc ccacttcgag tggcgggcgg
     4981 tgcgggggtg agagtgcgag gcctagcggc aaccccgtag cctcgcctcg tgtccggctt
     5041 gaggcctagc gtggtgtccg ccgccgcgtg ccactccggc cgcactatgc gttttttgtc
     5101 cttgctgccc tcgattgcct tccagcagca tgggctaaca aagggagggt gtggggctca
     5161 ctcttaagga gcccatgaag cttacgttgg ataggaatgg aagggcagga ggggcgactg
     5221 gggcccgccc gccttcggag cacatgtccg acgccacctg gatggggcga ggcctgtggc
     5281 tttccgaagc aatcgggcgt gagtttagcc tacctgggcc atgtggccct agcactgggc
     5341 acggtctggc ctggcggtgc cgcgttccct tgcctcccaa caagggtgag gccgtcccgc
     5401 ccggcaccag ttgcttgcgc ggaaagatgg ccgctcccgg ggccctgttg caaggagctc
     5461 aaaatggagg acgcggcagc ccggtggagc gggcgggtga gtcacccaca caaaggaaga
     5521 gggccttgcc cctcgccggc cgctgcttcc tgtgaccccg tggtctatcg gccgcatagt
     5581 cacctcgggc ttctcttgag caccgctcgt cgcggcgggg ggaggggatc taatggcgtt
     5641 ggagtttgtt cacatttggt gggtggagac tagtcaggcc agcctggcgc tggaagtcat
     5701 tcttggaatt tgcccctttg agtttggagc gaggctaatt ctcaagcctc ttagcggttc
     5761 aaaggtattt tctaaacccg tttccaggtg ttgtgaaagc caccgctaat tcaaagcaat
     5821 ccggagtata cggatccgcc accatgcggc acaagcggag caccaagaga gtgaagcggg
     5881 ccagcgccac ccagctgtac cggacctgca aagccgccgg aacctgcccc cctgacgtga
     5941 tccccaaggt ggagggcaac accgtggccg accagatcct gaagtacggc agcatggccg
     6001 tgttcttcgg cggcctgggc atcggcagcg gctctggcac cggcggcaga tccggctacg
     6061 tgcccctggg caccacacct cccaccgccg ccaccaacat tcccatcaga ccccccgtga
     6121 ccgtggagag catccccctg gacaccatcg gccccctgga ctccagcatc gtgtccctgg
     6181 tggaggaaac cagcttcatc gagagcggag cccccgtggt cacccccaga gtgcctccca
     6241 ctaccggctt caccatcacc acctccaccg acacaacccc cgccatcctg gacgtgacca
     6301 gcatcagcac ccacgacaac cccactttca ccgatcccag cgtgctgcac ccccccaccc
     6361 ctgccgagac aagcggccac ttcgtgctgt ccagcagcag catctccacc cacaactacg
     6421 aggaaatccc catggatacc ttcatcgtgt ccaccgacag caacaacatc accaacagca
     6481 cccccatccc cggcagccgg cccaccaccc ggctgggcct gtacagcaag ggcacccagc
     6541 aggtcaaggt ggtggacccc gccttcatga ccagccccgc caagctgatc acctacgaca
     6601 atcccgccta cgagggcctg aaccccgata ccaccctgca gttcgagcac gaggacatca
     6661 gcctggcccc cgaccccgat ttcatggaca tcattgccct gcacagaccc gccctgacct
     6721 ctcggaaggg caccatccgg tacagcagag tgggcaacaa gcggaccatg cacaccagaa
     6781 gcggcaaggc catcggcgcc agagtgcact actaccagga cctgagcagc atcaccgagg
     6841 acattgagct gcagcccctg cagcacgtgc ccagcagcct gcctcacacc accgtgtcca
     6901 cctccctgaa cgacggcatg ttcgacatct acgcccccat cgacaccgaa gaggacatca
     6961 tcttcagcgc cagctccaac aacaccctgt acaccacaag caacaccgcc tacgtgccct
     7021 ccaacaccac catccccctg agcagcggct acgacatccc tatcaccgcc ggacccgaca
     7081 tcgtgttcaa cagcaatacc atcacaaaca ccgtgctgcc cgtgcccacc ggccccatct
     7141 acagcatcat tgccgacggc ggcgacttct acctgcaccc cagctactac ctgctgaagc
     7201 ggcggagaaa gagaatcccc tacttcttcg ccgacgtgtc cgtggccgtg tgatagtcta
     7261 ggagcaggtt tccccaatga cacaaaacgt gcaacttgaa actccgcctg gtctttccag
     7321 gtctagaggg gtaacacttt gtactgcgtt tggctccacg ctcgatccac tggcgagtgt
     7381 tagtaacagc actgttgctt cgtagcggag catgacggcc gtgggaactc ctccttggta
     7441 acaaggaccc acggggccaa aagccacgcc cacacgggcc cgtcatgtgt gcaaccccag
     7501 cacggcgact ttactgcgaa acccacttta aagtgacatt gaaactggta cccacacact
     7561 ggtgacaggc taaggatgcc cttcaggtac cccgaggtaa cacgcgacac tcgggatctg
     7621 agaaggggac tggggcttct ataaaagcgc tcggtttaaa aagcttctat gcctgaatag
     7681 gtgaccggag gtcggcacct ttcctttgca attactgacc ctatgaatac actgactgtt
     7741 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgactcac tataggaggg
     7801 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     7861 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     7921 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     7981 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     8041 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     8101 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     8161 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     8221 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     8281 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     8341 ccgacggcga ggatctcgtc gtgacacatg gcgatgcctg cttgccgaat atcatggtgg
     8401 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     8461 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     8521 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     8581 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgaattc
     8641 gctaggatta tccctaatac ctgccacccc actcttaatc agtggtggaa gaacggtctc
     8701 agaactgttt gtttcaattg gccatttaag tttagtagta aaagactggt taatgataac
     8761 aatgcatcgt aaaaccttca gaaggaaagg agaatgtttt gtggaccact ttggttttct
     8821 tttttgcgtg tggcagtttt aagttattag tttttaaaat cagtactttt taatggaaac
     8881 aacttgacca aaaatttgtc acagaatttt gagacccatt aaaaaagtta aatgagaaac
     8941 ctgtgtgttc ctttggtcaa caccgagaca tttaggtgaa agacatctaa ttctggtttt
     9001 acgaatctgg aaacttcttg aaaatgtaat tcttgagtta acacttctgg gtggagaata
     9061 gggttgtttt ccccccacat aattggaagg ggaaggaata tcatttaaag ctatgggagg
     9121 gttgctttga ttacaacact ggagagaaat gcagcatgtt gctgattgcc tgtcactaaa
     9181 acaggccaaa aactgagtcc ttgggttgca tagaaagctg
//
