LOCUS       sequence-81432-e       15465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses eNpHR 3.0-EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15465)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4Halo3Y
FEATURES             Location/Qualifiers
     source          1..15465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(5637..5639,5640..6350)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5679..5700)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5940..5959)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6287..6308
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8126..8137
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13137..13155)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13151..13198
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13221..13237)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13221..13237)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13274..13294)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13274..13292)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13313..13329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13313..13329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13326..13348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13337..13353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13361..13367,13368..13385,13386..13391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13406..13427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13544..13561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13715..14303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13795..13814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14474..15265,15266..15334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15097..15116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15335..15439)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatgaca gagaccctgc ctcccgtgac cgagagtgcc gtggcccttc
     4741 aagccgaggt tacccaaagg gagttgttcg agttcgtgct gaacgaccct ttgcttgcaa
     4801 gcagtctcta tatcaacatc gcacttgcag gactgagtat actgctgttc gtttttatga
     4861 cccgaggact cgatgatcca cgggcaaaac ttattgctgt gtcaaccatc cttgtgcctg
     4921 tcgtcagcat tgcctcctac actggattgg cgagcggcct gacaatttcc gttcttgaaa
     4981 tgccagcggg ccattttgca gaaggcagct cagtgatgct gggaggagaa gaggtagatg
     5041 gtgtagtcac catgtgggga cggtatctca cctgggcact ttccacgccc atgattctcc
     5101 tcgctctggg tctcctggcc ggaagcaatg ctacaaagct cttcacagct atcactttcg
     5161 atatcgctat gtgcgtgact ggccttgccg cggccctgac tacctcctcc cacctcatga
     5221 gatggttctg gtacgctatc agttgtgcat gctttctggt ggtcttgtat atcctgctgg
     5281 tggagtgggc acaggacgcc aaagccgcgg gaaccgctga catgttcaat accctgaagc
     5341 tgttgacagt agtgatgtgg ctggggtatc caattgtgtg ggctcttgga gtcgagggta
     5401 tcgcggtgtt gcccgttggg gtgacgagct ggggatattc tttcctggat atcgtggcaa
     5461 agtacatttt cgcattcttg ctcctgaact atctgacgtc aaacgaatct gtcgtgtccg
     5521 gcagcatttt ggatgttcca tctgcttctg ggaccccggc tgatgatgcg gccgccaaga
     5581 gcaggatcac cagcgagggc gagtacatcc ccctggacca gatcgacatc aacgtggtga
     5641 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     5701 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     5761 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     5821 ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     5881 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     5941 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     6001 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     6061 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     6121 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     6181 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6241 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6301 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ttctgctacg
     6361 agaacgaggt gtaatgaccc gggttataaa gtgctgggtc atctaagctt ttcagtcgag
     6421 aaaaaaacat tagatcagaa gaacaactgg caacacttct caacctgaga cttacttcaa
     6481 gatgctcgat cctggagagg tctatgatga ccctattgac ccaatcgagt tagaggctga
     6541 acccagagga acccccattg tccccaacat cttgaggaac tctgactaca atctcaactc
     6601 tcctttgata gaagatcctg ctagactaat gttagaatgg ttaaaaacag ggaatagacc
     6661 ttatcggatg actctaacag acaattgctc caggtctttc agagttttga aagattattt
     6721 caagaaggta gatttgggtt ctctcaaggt gggcggaatg gctgcacagt caatgatttc
     6781 tctctggtta tatggtgccc actctgaatc caacaggagc cggagatgta taacagactt
     6841 ggcccatttc tattccaagt cgtcccccat agagaagctg ttgaatctca cgctaggaaa
     6901 tagagggctg agaatccccc cagagggagt gttaagttgc cttgagaggg ttgattatga
     6961 taatgcattt ggaaggtatc ttgccaacac gtattcctct tacttgttct tccatgtaat
     7021 caccttatac atgaacgccc tagactggga tgaagaaaag accatcctag cattatggaa
     7081 agatttaacc tcagtggaca tcgggaagga cttggtaaag ttcaaagacc aaatatgggg
     7141 actgctgatc gtgacaaagg actttgttta ctcccaaagt tccaattgtc tttttgacag
     7201 aaactacaca cttatgctaa aagatctttt cttgtctcgc ttcaactcct taatggtctt
     7261 gctctctccc ccagagcccc gatactcaga tgacttgata tctcaactat gccagctgta
     7321 cattgctggg gatcaagtct tgtctatgtg tggaaactcc ggctatgaag tcatcaaaat
     7381 attggagcca tatgtcgtga atagtttagt ccagagagca gaaaagttta ggcctctcat
     7441 tcattccttg ggagactttc ctgtatttat aaaagacaag gtaagtcaac ttgaagagac
     7501 gttcggtccc tgtgcaagaa ggttctttag ggctctggat caattcgaca acatacatga
     7561 cttggttttt gtgtttggct gttacaggca ttgggggcac ccatatatag attatcgaaa
     7621 gggtctgtca aaactatatg atcaggttca ccttaaaaaa atgatagata agtcctacca
     7681 ggagtgctta gcaagcgacc tagccaggag gatccttaga tggggttttg ataagtactc
     7741 caagtggtat ctggattcaa gattcctagc ccgagaccac cccttgactc cttatatcaa
     7801 aacccaaaca tggccaccca aacatattgt agacttggtg ggggatacat ggcacaagct
     7861 cccgatcacg cagatctttg agattcctga atcaatggat ccgtcagaaa tattggatga
     7921 caaatcacat tctttcacca gaacgagact agcttcttgg ctgtcagaaa accgaggggg
     7981 gcctgttcct agcgaaaaag ttattatcac ggccctgtct aagccgcctg tcaatccccg
     8041 agagtttctg aggtctatag acctcggagg attgccagat gaagacttga taattggcct
     8101 caagccaaag gaacgggaat tgaagattga aggtcgattc tttgctctaa tgtcatggaa
     8161 tctaagattg tattttgtca tcactgaaaa actcttggcc aactacatct tgccactttt
     8221 tgacgcgctg actatgacag acaacctgaa caaggtgttt aaaaagctga tcgacagggt
     8281 caccgggcaa gggcttttgg actattcaag ggtcacatat gcatttcacc tggactatga
     8341 aaagtggaac aaccatcaaa gattagagtc aacagaggat gtattttctg tcctagatca
     8401 agtgtttgga ttgaagagag tgttttctag aacacacgag ttttttcaaa aggcctggat
     8461 ctattattca gacagatcag acctcatcgg gttacgggag gatcaaatat actgcttaga
     8521 tgcgtccaac ggcccaacct gttggaatgg ccaggatggc gggctagaag gcttacggca
     8581 gaagggctgg agtctagtca gcttattgat gatagataga gaatctcaaa tcaggaacac
     8641 aagaaccaaa atactagctc aaggagacaa ccaggtttta tgtccgacat acatgttgtc
     8701 gccagggcta tctcaagagg ggctcctcta tgaattggag agaatatcaa ggaatgcact
     8761 ttcgatatac agagccgtcg aggaaggggc atctaagcta gggctgatca tcaagaaaga
     8821 agagaccatg tgtagttatg acttcctcat ctatggaaaa acccctttgt ttagaggtaa
     8881 catattggtg cctgagtcca aaagatgggc cagagtctct tgcgtctcta atgaccaaat
     8941 agtcaacctc gccaatataa tgtcgacagt gtccaccaat gcgctaacag tggcacaaca
     9001 ctctcaatct ttgatcaaac cgatgaggga ttttctgctc atgtcagtac aggcagtctt
     9061 tcactacctg ctatttagcc caatcttaaa gggaagagtt tacaagattc tgagcgctga
     9121 aggggagagc tttctcctag ccatgtcaag gataatctat ctagatcctt ctttgggagg
     9181 gatatctgga atgtccctcg gaagattcca tatacgacag ttctcagacc ctgtctctga
     9241 agggttatcc ttctggagag agatctggtt aagctcccaa gagtcctgga ttcacgcgtt
     9301 gtgtcaagag gctggaaacc cagatcttgg agagagaaca ctcgagagct tcactcgcct
     9361 tctagaagat ccgaccacct taaatatcag aggaggggcc agtcctacca ttctactcaa
     9421 ggatgcaatc agaaaggctt tatatgacga ggtggacaag gtggaaaatt cagagtttcg
     9481 agaggcaatc ctgttgtcca agacccatag agataatttt atactcttct taatatctgt
     9541 tgagcctctg tttcctcgat ttctcagtga gctattcagt tcgtcttttt tgggaatccc
     9601 cgagtcaatc attggattga tacaaaactc ccgaacgata agaaggcagt ttagaaagag
     9661 tctctcaaaa actttagaag aatccttcta caactcagag atccacggga ttagtcggat
     9721 gacccagaca cctcagaggg ttgggggggt gtggccttgc tcttcagaga gggcagatct
     9781 acttagggag atctcttggg gaagaaaagt ggtaggcacg acagttcctc acccttctga
     9841 gatgttggga ttacttccca agtcctctat ttcttgcact tgtggagcaa caggaggagg
     9901 caatcctaga gtttctgtat cagtactccc gtcctttgat cagtcatttt tttcacgagg
     9961 ccccctaaag ggatacttgg gctcgtccac ctctatgtcg acccagctat tccatgcatg
    10021 ggaaaaagtc actaatgttc atgtggtgaa gagagctcta tcgttaaaag aatctataaa
    10081 ctggttcatt actagagatt ccaacttggc tcaagctcta attaggaaca ttatgtctct
    10141 gacaggccct gatttccctc tagaggaggc ccctgtcttc aaaaggacgg ggtcagcctt
    10201 gcataggttc aagtctgcca gatacagcga aggagggtat tcttctgtct gcccgaacct
    10261 cctctctcat atttctgtta gtacagacac catgtctgat ttgacccaag acgggaagaa
    10321 ctacgatttc atgttccagc cattgatgct ttatgcacag acatggacat cagagctggt
    10381 acagagagac acaaggctaa gagactctac gtttcattgg cacctccgat gcaacaggtg
    10441 tgtgagaccc attgacgacg tgaccctgga gacctctcag atcttcgagt ttccggatgt
    10501 gtcgaaaaga atatccagaa tggtttctgg ggctgtgcct cacttccaga ggcttcccga
    10561 tatccgtctg agaccaggag attttgaatc tctaagcggt agagaaaagt ctcaccatat
    10621 cggatcagct caggggctct tatactcaat cttagtggca attcacgact caggatacaa
    10681 tgatggaacc atcttccctg tcaacatata cggcaaggtt tcccctagag actatttgag
    10741 agggctcgca aggggagtat tgataggatc ctcgatttgc ttcttgacaa gaatgacaaa
    10801 tatcaatatt aatagacctc ttgaattggt ctcaggggta atctcatata ttctcctgag
    10861 gctagataac catccctcct tgtacataat gctcagagaa ccgtctctta gaggagagat
    10921 attttctatc cctcagaaaa tccccgccgc ttatccaacc actatgaaag aaggcaacag
    10981 atcaatcttg tgttatctcc aacatgtgct acgctatgag cgagagataa tcacggcgtc
    11041 tccagagaat gactggctat ggatcttttc agactttaga agtgccaaaa tgacgtacct
    11101 atccctcatt acttaccagt ctcatcttct actccagagg gttgagagaa acctatctaa
    11161 gagtatgaga gataacctgc gacaattgag ttctttgatg aggcaggtgc tgggcgggca
    11221 cggagaagat accttagagt cagacgacaa cattcaacga ctgctaaaag actctttacg
    11281 aaggacaaga tgggtggatc aagaggtgcg ccatgcagct agaaccatga ctggagatta
    11341 cagccccaac aagaaggtgt cccgtaaggt aggatgttca gaatgggtct gctctgctca
    11401 acaggttgca gtctctacct cagcaaaccc ggcccctgtc tcggagcttg acataagggc
    11461 cctctctaag aggttccaga accctttgat ctcgggcttg agagtggttc agtgggcaac
    11521 cggtgctcat tataagctta agcctattct agatgatctc aatgttttcc catctctctg
    11581 ccttgtagtt ggggacgggt caggggggat atcaagggca gtcctcaaca tgtttccaga
    11641 tgccaagctt gtgttcaaca gtcttttaga ggtgaatgac ctgatggctt ccggaacaca
    11701 tccactgcct ccttcagcaa tcatgagggg aggaaatgat atcgtctcca gagtgataga
    11761 tcttgactca atctgggaaa aaccgtccga cttgagaaac ttggcaacct ggaaatactt
    11821 ccagtcagtc caaaagcagg tcaacatgtc ctatgacctc attatttgcg atgcagaagt
    11881 tactgacatt gcatctatca accggatcac cctgttaatg tccgattttg cattgtctat
    11941 agatggacca ctctatttgg tcttcaaaac ttatgggact atgctagtaa atccaaacta
    12001 caaggctatt caacacctgt caagagcgtt cccctcggtc acagggttta tcacccaagt
    12061 aacttcgtct ttttcatctg agctctacct ccgattctcc aaacgaggga agtttttcag
    12121 agatgctgag tacttgacct cttccaccct tcgagaaatg agccttgtgt tattcaattg
    12181 tagcagcccc aagagtgaga tgcagagagc tcgttccttg aactatcagg atcttgtgag
    12241 aggatttcct gaagaaatca tatcaaatcc ttacaatgag atgatcataa ctctgattga
    12301 cagtgatgta gaatcttttc tagtccacaa gatggttgat gatcttgagt tacagagggg
    12361 aactctgtct aaagtggcta tcattatagc catcatgata gttttctcca acagagtctt
    12421 caacgtttcc aaacccctaa ctgacccctc gttctatcca ccgtctgatc ccaaaatcct
    12481 gaggcacttc aacatatgtt gcagtactat gatgtatcta tctactgctt taggtgacgt
    12541 ccctagcttc gcaagacttc acgacctgta taacagacct ataacttatt acttcagaaa
    12601 gcaagtcatt cgagggaacg tttatctatc ttggagttgg tccaacgaca cctcagtgtt
    12661 caaaagggta gcctgtaatt ctagcctgag tctgtcatct cactggatca ggttgattta
    12721 caagatagtg aagactacca gactcgttgg cagcatcaag gatctatcca gagaagtgga
    12781 aagacacctt cataggtaca acaggtggat caccctagag gatatcagat ctagatcatc
    12841 cctactagac tacagttgcc tgtgaaccgg atactcctgg aagcctgccc atgctaagac
    12901 tcttgtgtga tgtatcttga aaaaaacaag atcctaaatc tgaacctttg gttgtttgat
    12961 tgtttttctc atttttgttg tttatttgtt aagcgtgggt cggcatggca tctccacctc
    13021 ctcgcggtcc gacctgggca tccgaaggag gacgcacgtc cactcggatg gctaagggag
    13081 gggcggggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
    13141 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
    13201 gaaaggagga actatatccg gatccactag ttctagagcg gccgccaccg cggtggagct
    13261 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
    13321 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    13381 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    13441 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    13501 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    13561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    13621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    13681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    13741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    13801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    13861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    13921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    13981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    14041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    14101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    14161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    14221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    14281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    14341 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    14401 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    14461 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    14521 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    14581 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    14641 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    14701 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    14761 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    14821 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    14881 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    14941 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    15001 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    15061 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    15121 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    15181 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    15241 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    15301 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    15361 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    15421 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctg
//
