LOCUS       sequence-81433-e       15137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GCaMP6s.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15137)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4GCaMP6s
FEATURES             Location/Qualifiers
     source          1..15137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4695..4703,4704..4721,4722..4724,4725..4757,4758..4760,
                     4761..4784,4785..4811,4812..4868,4869..4874,4875..5144,
                     5145..5162,5163..5597,5598..5603,5604..6047)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      4791..4800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5081..5102
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5208..5229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5469..5488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7798..7809
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12809..12827)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12823..12870
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12893..12909)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12893..12909)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12946..12966)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12946..12964)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12985..13001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12985..13001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12998..13020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13009..13025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13033..13039,13040..13057,13058..13063))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13078..13099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13216..13233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13387..13975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13467..13486)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14146..14937,14938..15006))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14769..14788
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15007..15111)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatgggt tctcatcatc atcatcatca tggtatggct agcatgactg
     4741 gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatctc gccaccatgg
     4801 tcgactcatc acgtcgtaag tggaataaga caggtcacgc agtcagagct ataggtcggc
     4861 tgagctcact cgagaacgtc tatatcaagg ccgacaagca gaagaacggc atcaaggcga
     4921 acttccacat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac cactaccagc
     4981 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcgtgc
     5041 agtccaaact ttcgaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5101 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga
     5161 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5221 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc gatgccacct
     5281 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5341 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5401 agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag cgcaccatct
     5461 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5521 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5581 acaagctgga gtacaacctg ccggaccaac tgactgaaga gcagatcgca gaatttaaag
     5641 aggctttctc cctatttgac aaggacgggg atgggacaat aacaaccaag gagctgggga
     5701 cggtgatgcg gtctctgggg cagaacccca cagaagcaga gctgcaggac atgatcaatg
     5761 aagtagatgc cgacggtgac ggcacaatcg acttccctga gttcctgaca atgatggcaa
     5821 gaaaaatgaa atacagggac acggaagaag aaattagaga agcgttcggt gtgtttgata
     5881 aggatggcaa tggctacatc agtgcagcag agcttcgcca cgtgatgaca aaccttggag
     5941 agaagttaac agatgaagag gttgatgaaa tgatcaggga agcagacatc gatggggatg
     6001 gtcaggtaaa ctacgaagag tttgtacaaa tgatgacagc gaagtgatag ctagcttata
     6061 aagtgctggg tcatctaagc ttttcagtcg agaaaaaaac attagatcag aagaacaact
     6121 ggcaacactt ctcaacctga gacttacttc aagatgctcg atcctggaga ggtctatgat
     6181 gaccctattg acccaatcga gttagaggct gaacccagag gaacccccat tgtccccaac
     6241 atcttgagga actctgacta caatctcaac tctcctttga tagaagatcc tgctagacta
     6301 atgttagaat ggttaaaaac agggaataga ccttatcgga tgactctaac agacaattgc
     6361 tccaggtctt tcagagtttt gaaagattat ttcaagaagg tagatttggg ttctctcaag
     6421 gtgggcggaa tggctgcaca gtcaatgatt tctctctggt tatatggtgc ccactctgaa
     6481 tccaacagga gccggagatg tataacagac ttggcccatt tctattccaa gtcgtccccc
     6541 atagagaagc tgttgaatct cacgctagga aatagagggc tgagaatccc cccagaggga
     6601 gtgttaagtt gccttgagag ggttgattat gataatgcat ttggaaggta tcttgccaac
     6661 acgtattcct cttacttgtt cttccatgta atcaccttat acatgaacgc cctagactgg
     6721 gatgaagaaa agaccatcct agcattatgg aaagatttaa cctcagtgga catcgggaag
     6781 gacttggtaa agttcaaaga ccaaatatgg ggactgctga tcgtgacaaa ggactttgtt
     6841 tactcccaaa gttccaattg tctttttgac agaaactaca cacttatgct aaaagatctt
     6901 ttcttgtctc gcttcaactc cttaatggtc ttgctctctc ccccagagcc ccgatactca
     6961 gatgacttga tatctcaact atgccagctg tacattgctg gggatcaagt cttgtctatg
     7021 tgtggaaact ccggctatga agtcatcaaa atattggagc catatgtcgt gaatagttta
     7081 gtccagagag cagaaaagtt taggcctctc attcattcct tgggagactt tcctgtattt
     7141 ataaaagaca aggtaagtca acttgaagag acgttcggtc cctgtgcaag aaggttcttt
     7201 agggctctgg atcaattcga caacatacat gacttggttt ttgtgtttgg ctgttacagg
     7261 cattgggggc acccatatat agattatcga aagggtctgt caaaactata tgatcaggtt
     7321 caccttaaaa aaatgataga taagtcctac caggagtgct tagcaagcga cctagccagg
     7381 aggatcctta gatggggttt tgataagtac tccaagtggt atctggattc aagattccta
     7441 gcccgagacc accccttgac tccttatatc aaaacccaaa catggccacc caaacatatt
     7501 gtagacttgg tgggggatac atggcacaag ctcccgatca cgcagatctt tgagattcct
     7561 gaatcaatgg atccgtcaga aatattggat gacaaatcac attctttcac cagaacgaga
     7621 ctagcttctt ggctgtcaga aaaccgaggg gggcctgttc ctagcgaaaa agttattatc
     7681 acggccctgt ctaagccgcc tgtcaatccc cgagagtttc tgaggtctat agacctcgga
     7741 ggattgccag atgaagactt gataattggc ctcaagccaa aggaacggga attgaagatt
     7801 gaaggtcgat tctttgctct aatgtcatgg aatctaagat tgtattttgt catcactgaa
     7861 aaactcttgg ccaactacat cttgccactt tttgacgcgc tgactatgac agacaacctg
     7921 aacaaggtgt ttaaaaagct gatcgacagg gtcaccgggc aagggctttt ggactattca
     7981 agggtcacat atgcatttca cctggactat gaaaagtgga acaaccatca aagattagag
     8041 tcaacagagg atgtattttc tgtcctagat caagtgtttg gattgaagag agtgttttct
     8101 agaacacacg agttttttca aaaggcctgg atctattatt cagacagatc agacctcatc
     8161 gggttacggg aggatcaaat atactgctta gatgcgtcca acggcccaac ctgttggaat
     8221 ggccaggatg gcgggctaga aggcttacgg cagaagggct ggagtctagt cagcttattg
     8281 atgatagata gagaatctca aatcaggaac acaagaacca aaatactagc tcaaggagac
     8341 aaccaggttt tatgtccgac atacatgttg tcgccagggc tatctcaaga ggggctcctc
     8401 tatgaattgg agagaatatc aaggaatgca ctttcgatat acagagccgt cgaggaaggg
     8461 gcatctaagc tagggctgat catcaagaaa gaagagacca tgtgtagtta tgacttcctc
     8521 atctatggaa aaaccccttt gtttagaggt aacatattgg tgcctgagtc caaaagatgg
     8581 gccagagtct cttgcgtctc taatgaccaa atagtcaacc tcgccaatat aatgtcgaca
     8641 gtgtccacca atgcgctaac agtggcacaa cactctcaat ctttgatcaa accgatgagg
     8701 gattttctgc tcatgtcagt acaggcagtc tttcactacc tgctatttag cccaatctta
     8761 aagggaagag tttacaagat tctgagcgct gaaggggaga gctttctcct agccatgtca
     8821 aggataatct atctagatcc ttctttggga gggatatctg gaatgtccct cggaagattc
     8881 catatacgac agttctcaga ccctgtctct gaagggttat ccttctggag agagatctgg
     8941 ttaagctccc aagagtcctg gattcacgcg ttgtgtcaag aggctggaaa cccagatctt
     9001 ggagagagaa cactcgagag cttcactcgc cttctagaag atccgaccac cttaaatatc
     9061 agaggagggg ccagtcctac cattctactc aaggatgcaa tcagaaaggc tttatatgac
     9121 gaggtggaca aggtggaaaa ttcagagttt cgagaggcaa tcctgttgtc caagacccat
     9181 agagataatt ttatactctt cttaatatct gttgagcctc tgtttcctcg atttctcagt
     9241 gagctattca gttcgtcttt tttgggaatc cccgagtcaa tcattggatt gatacaaaac
     9301 tcccgaacga taagaaggca gtttagaaag agtctctcaa aaactttaga agaatccttc
     9361 tacaactcag agatccacgg gattagtcgg atgacccaga cacctcagag ggttgggggg
     9421 gtgtggcctt gctcttcaga gagggcagat ctacttaggg agatctcttg gggaagaaaa
     9481 gtggtaggca cgacagttcc tcacccttct gagatgttgg gattacttcc caagtcctct
     9541 atttcttgca cttgtggagc aacaggagga ggcaatccta gagtttctgt atcagtactc
     9601 ccgtcctttg atcagtcatt tttttcacga ggccccctaa agggatactt gggctcgtcc
     9661 acctctatgt cgacccagct attccatgca tgggaaaaag tcactaatgt tcatgtggtg
     9721 aagagagctc tatcgttaaa agaatctata aactggttca ttactagaga ttccaacttg
     9781 gctcaagctc taattaggaa cattatgtct ctgacaggcc ctgatttccc tctagaggag
     9841 gcccctgtct tcaaaaggac ggggtcagcc ttgcataggt tcaagtctgc cagatacagc
     9901 gaaggagggt attcttctgt ctgcccgaac ctcctctctc atatttctgt tagtacagac
     9961 accatgtctg atttgaccca agacgggaag aactacgatt tcatgttcca gccattgatg
    10021 ctttatgcac agacatggac atcagagctg gtacagagag acacaaggct aagagactct
    10081 acgtttcatt ggcacctccg atgcaacagg tgtgtgagac ccattgacga cgtgaccctg
    10141 gagacctctc agatcttcga gtttccggat gtgtcgaaaa gaatatccag aatggtttct
    10201 ggggctgtgc ctcacttcca gaggcttccc gatatccgtc tgagaccagg agattttgaa
    10261 tctctaagcg gtagagaaaa gtctcaccat atcggatcag ctcaggggct cttatactca
    10321 atcttagtgg caattcacga ctcaggatac aatgatggaa ccatcttccc tgtcaacata
    10381 tacggcaagg tttcccctag agactatttg agagggctcg caaggggagt attgatagga
    10441 tcctcgattt gcttcttgac aagaatgaca aatatcaata ttaatagacc tcttgaattg
    10501 gtctcagggg taatctcata tattctcctg aggctagata accatccctc cttgtacata
    10561 atgctcagag aaccgtctct tagaggagag atattttcta tccctcagaa aatccccgcc
    10621 gcttatccaa ccactatgaa agaaggcaac agatcaatct tgtgttatct ccaacatgtg
    10681 ctacgctatg agcgagagat aatcacggcg tctccagaga atgactggct atggatcttt
    10741 tcagacttta gaagtgccaa aatgacgtac ctatccctca ttacttacca gtctcatctt
    10801 ctactccaga gggttgagag aaacctatct aagagtatga gagataacct gcgacaattg
    10861 agttctttga tgaggcaggt gctgggcggg cacggagaag ataccttaga gtcagacgac
    10921 aacattcaac gactgctaaa agactcttta cgaaggacaa gatgggtgga tcaagaggtg
    10981 cgccatgcag ctagaaccat gactggagat tacagcccca acaagaaggt gtcccgtaag
    11041 gtaggatgtt cagaatgggt ctgctctgct caacaggttg cagtctctac ctcagcaaac
    11101 ccggcccctg tctcggagct tgacataagg gccctctcta agaggttcca gaaccctttg
    11161 atctcgggct tgagagtggt tcagtgggca accggtgctc attataagct taagcctatt
    11221 ctagatgatc tcaatgtttt cccatctctc tgccttgtag ttggggacgg gtcagggggg
    11281 atatcaaggg cagtcctcaa catgtttcca gatgccaagc ttgtgttcaa cagtctttta
    11341 gaggtgaatg acctgatggc ttccggaaca catccactgc ctccttcagc aatcatgagg
    11401 ggaggaaatg atatcgtctc cagagtgata gatcttgact caatctggga aaaaccgtcc
    11461 gacttgagaa acttggcaac ctggaaatac ttccagtcag tccaaaagca ggtcaacatg
    11521 tcctatgacc tcattatttg cgatgcagaa gttactgaca ttgcatctat caaccggatc
    11581 accctgttaa tgtccgattt tgcattgtct atagatggac cactctattt ggtcttcaaa
    11641 acttatggga ctatgctagt aaatccaaac tacaaggcta ttcaacacct gtcaagagcg
    11701 ttcccctcgg tcacagggtt tatcacccaa gtaacttcgt ctttttcatc tgagctctac
    11761 ctccgattct ccaaacgagg gaagtttttc agagatgctg agtacttgac ctcttccacc
    11821 cttcgagaaa tgagccttgt gttattcaat tgtagcagcc ccaagagtga gatgcagaga
    11881 gctcgttcct tgaactatca ggatcttgtg agaggatttc ctgaagaaat catatcaaat
    11941 ccttacaatg agatgatcat aactctgatt gacagtgatg tagaatcttt tctagtccac
    12001 aagatggttg atgatcttga gttacagagg ggaactctgt ctaaagtggc tatcattata
    12061 gccatcatga tagttttctc caacagagtc ttcaacgttt ccaaacccct aactgacccc
    12121 tcgttctatc caccgtctga tcccaaaatc ctgaggcact tcaacatatg ttgcagtact
    12181 atgatgtatc tatctactgc tttaggtgac gtccctagct tcgcaagact tcacgacctg
    12241 tataacagac ctataactta ttacttcaga aagcaagtca ttcgagggaa cgtttatcta
    12301 tcttggagtt ggtccaacga cacctcagtg ttcaaaaggg tagcctgtaa ttctagcctg
    12361 agtctgtcat ctcactggat caggttgatt tacaagatag tgaagactac cagactcgtt
    12421 ggcagcatca aggatctatc cagagaagtg gaaagacacc ttcataggta caacaggtgg
    12481 atcaccctag aggatatcag atctagatca tccctactag actacagttg cctgtgaacc
    12541 ggatactcct ggaagcctgc ccatgctaag actcttgtgt gatgtatctt gaaaaaaaca
    12601 agatcctaaa tctgaacctt tggttgtttg attgtttttc tcatttttgt tgtttatttg
    12661 ttaagcgtgg gtcggcatgg catctccacc tcctcgcggt ccgacctggg catccgaagg
    12721 aggacgcacg tccactcgga tggctaaggg aggggcgggg atccggctgc taacaaagcc
    12781 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
    12841 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatccact
    12901 agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
    12961 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    13021 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    13081 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    13141 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    13201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    13261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    13321 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    13381 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    13441 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    13501 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    13561 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    13621 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    13681 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    13741 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    13801 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    13861 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    13921 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    13981 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    14041 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    14101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    14161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    14221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    14281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    14341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    14401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    14461 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    14521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    14581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    14641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    14701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    14761 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    14821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    14881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    14941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    15001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    15061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    15121 ccgaaaagtg ccacctg
//
