LOCUS       sequence-81435-e       16017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mTagBFP2 and postsynaptic mGRASP component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16017)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4BFP2-5postmGRASP
FEATURES             Location/Qualifiers
     source          1..16017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4695..5396
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     regulatory      5466..5475
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(6534..6545)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8678..8689
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13689..13707)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13703..13750
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13773..13789)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13773..13789)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13826..13846)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13826..13844)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13865..13881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13865..13881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13878..13900)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13889..13905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13913..13919,13920..13937,13938..13943))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13958..13979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14096..14113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14267..14855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14347..14366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15026..15817,15818..15886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15649..15668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15887..15991)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
     4741 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
     4801 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     4861 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
     4921 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     4981 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     5041 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     5101 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     5161 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga
     5221 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     5281 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     5341 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aattaatgat
     5401 taattaacgt cctttcaacg atccaagtcc atgaaaaaaa ctaacacccc tcccgtacga
     5461 aagccgccac catggcactt cctagatgta tgtggcctaa ttatgtatgg cgagctatga
     5521 tggcctgcgt ggtgcaccga gggagtggcg ccccacttac actctgcctg ttgggttgcc
     5581 tcctccagac tttccacgtt ctttcacaga agctggatga catgtccaag ggagaagagt
     5641 tgtttactgg cgtagtccct attctcgtgg aactcgatgg tgacgtgaat ggccataagt
     5701 tttctgtcag aggagaggga gaaggcgatg ccaccatcgg gaaactcacg ctgaaattca
     5761 tctgtaccac tggaaaactt cccgtgcctt ggccaaccct cgtgacaaca ctcacctacg
     5821 gggtgcaatg tttctctcgg tacccggacc atatgaagag gcatgacttc ttcaagagtg
     5881 ccatgcccga gggttacgtt caggagcgca ccatctcttt taaggacgat ggcaaatata
     5941 agacaagagc agtcgtcaag ttcgagggtg atacactcgt taaccgcatc gagctcaaag
     6001 gcaccgattt taaggaggac ggaaatatcc tgggacacaa attggagtac aacttcaaca
     6061 gtcacaacgt gtatattaca gcagataagc agaagaatgg cataaaggcc aatttcacgg
     6121 taagacataa tgtcgaggat ggcagtgtcc agctggcaga ccactaccag caaaacacgc
     6181 ccattggcga tggacctgtt ctcctgccag acaaccacta cctcagtacc caaacagtcc
     6241 tgtccaagga ccctaatgag aaagggacgc tcgagctggt gccccacttg cataacctca
     6301 atgacatcag ccaatacaca agtacaacca ccaaggtccc aagcactgac atcacccttc
     6361 gaccaactcg gaaaaactca acccctgtga ccagcgcatt tccaaccgct aagcaagacg
     6421 accccaaaca acagcctagt ccatttagcg tagaccaaag ggactacagc acagagctgt
     6481 ccgtgaccat agctgtagga gcttccctgc ttttcctgaa cattctggct ttcgcggccc
     6541 tctattacaa gaaggacaaa aggcgccacg acgtacatag gagatgtagt ccgcaacgaa
     6601 ctacaaccaa tgatcttacg cacgcacctg aggaggagat catgagtctt cagatgaaac
     6661 acacagacct ggaccacgag tgtgaatcca tccatcccca tgaggtggtg ctgaggacag
     6721 catgccctcc ggactatacc ctggcaatgc gccgaagccc agacgacatt cccctgatga
     6781 cacccaatac cataaccatg attcccaaca ctattccagg aatccagcct ctccacactt
     6841 tcaacacatt cactggtgga cagaataata ctctccctca tccgcatcca cacccccact
     6901 cccacagcac aacacgggtg ggttaatgag ctagcttata aagtgctggg tcatctaagc
     6961 ttttcagtcg agaaaaaaac attagatcag aagaacaact ggcaacactt ctcaacctga
     7021 gacttacttc aagatgctcg atcctggaga ggtctatgat gaccctattg acccaatcga
     7081 gttagaggct gaacccagag gaacccccat tgtccccaac atcttgagga actctgacta
     7141 caatctcaac tctcctttga tagaagatcc tgctagacta atgttagaat ggttaaaaac
     7201 agggaataga ccttatcgga tgactctaac agacaattgc tccaggtctt tcagagtttt
     7261 gaaagattat ttcaagaagg tagatttggg ttctctcaag gtgggcggaa tggctgcaca
     7321 gtcaatgatt tctctctggt tatatggtgc ccactctgaa tccaacagga gccggagatg
     7381 tataacagac ttggcccatt tctattccaa gtcgtccccc atagagaagc tgttgaatct
     7441 cacgctagga aatagagggc tgagaatccc cccagaggga gtgttaagtt gccttgagag
     7501 ggttgattat gataatgcat ttggaaggta tcttgccaac acgtattcct cttacttgtt
     7561 cttccatgta atcaccttat acatgaacgc cctagactgg gatgaagaaa agaccatcct
     7621 agcattatgg aaagatttaa cctcagtgga catcgggaag gacttggtaa agttcaaaga
     7681 ccaaatatgg ggactgctga tcgtgacaaa ggactttgtt tactcccaaa gttccaattg
     7741 tctttttgac agaaactaca cacttatgct aaaagatctt ttcttgtctc gcttcaactc
     7801 cttaatggtc ttgctctctc ccccagagcc ccgatactca gatgacttga tatctcaact
     7861 atgccagctg tacattgctg gggatcaagt cttgtctatg tgtggaaact ccggctatga
     7921 agtcatcaaa atattggagc catatgtcgt gaatagttta gtccagagag cagaaaagtt
     7981 taggcctctc attcattcct tgggagactt tcctgtattt ataaaagaca aggtaagtca
     8041 acttgaagag acgttcggtc cctgtgcaag aaggttcttt agggctctgg atcaattcga
     8101 caacatacat gacttggttt ttgtgtttgg ctgttacagg cattgggggc acccatatat
     8161 agattatcga aagggtctgt caaaactata tgatcaggtt caccttaaaa aaatgataga
     8221 taagtcctac caggagtgct tagcaagcga cctagccagg aggatcctta gatggggttt
     8281 tgataagtac tccaagtggt atctggattc aagattccta gcccgagacc accccttgac
     8341 tccttatatc aaaacccaaa catggccacc caaacatatt gtagacttgg tgggggatac
     8401 atggcacaag ctcccgatca cgcagatctt tgagattcct gaatcaatgg atccgtcaga
     8461 aatattggat gacaaatcac attctttcac cagaacgaga ctagcttctt ggctgtcaga
     8521 aaaccgaggg gggcctgttc ctagcgaaaa agttattatc acggccctgt ctaagccgcc
     8581 tgtcaatccc cgagagtttc tgaggtctat agacctcgga ggattgccag atgaagactt
     8641 gataattggc ctcaagccaa aggaacggga attgaagatt gaaggtcgat tctttgctct
     8701 aatgtcatgg aatctaagat tgtattttgt catcactgaa aaactcttgg ccaactacat
     8761 cttgccactt tttgacgcgc tgactatgac agacaacctg aacaaggtgt ttaaaaagct
     8821 gatcgacagg gtcaccgggc aagggctttt ggactattca agggtcacat atgcatttca
     8881 cctggactat gaaaagtgga acaaccatca aagattagag tcaacagagg atgtattttc
     8941 tgtcctagat caagtgtttg gattgaagag agtgttttct agaacacacg agttttttca
     9001 aaaggcctgg atctattatt cagacagatc agacctcatc gggttacggg aggatcaaat
     9061 atactgctta gatgcgtcca acggcccaac ctgttggaat ggccaggatg gcgggctaga
     9121 aggcttacgg cagaagggct ggagtctagt cagcttattg atgatagata gagaatctca
     9181 aatcaggaac acaagaacca aaatactagc tcaaggagac aaccaggttt tatgtccgac
     9241 atacatgttg tcgccagggc tatctcaaga ggggctcctc tatgaattgg agagaatatc
     9301 aaggaatgca ctttcgatat acagagccgt cgaggaaggg gcatctaagc tagggctgat
     9361 catcaagaaa gaagagacca tgtgtagtta tgacttcctc atctatggaa aaaccccttt
     9421 gtttagaggt aacatattgg tgcctgagtc caaaagatgg gccagagtct cttgcgtctc
     9481 taatgaccaa atagtcaacc tcgccaatat aatgtcgaca gtgtccacca atgcgctaac
     9541 agtggcacaa cactctcaat ctttgatcaa accgatgagg gattttctgc tcatgtcagt
     9601 acaggcagtc tttcactacc tgctatttag cccaatctta aagggaagag tttacaagat
     9661 tctgagcgct gaaggggaga gctttctcct agccatgtca aggataatct atctagatcc
     9721 ttctttggga gggatatctg gaatgtccct cggaagattc catatacgac agttctcaga
     9781 ccctgtctct gaagggttat ccttctggag agagatctgg ttaagctccc aagagtcctg
     9841 gattcacgcg ttgtgtcaag aggctggaaa cccagatctt ggagagagaa cactcgagag
     9901 cttcactcgc cttctagaag atccgaccac cttaaatatc agaggagggg ccagtcctac
     9961 cattctactc aaggatgcaa tcagaaaggc tttatatgac gaggtggaca aggtggaaaa
    10021 ttcagagttt cgagaggcaa tcctgttgtc caagacccat agagataatt ttatactctt
    10081 cttaatatct gttgagcctc tgtttcctcg atttctcagt gagctattca gttcgtcttt
    10141 tttgggaatc cccgagtcaa tcattggatt gatacaaaac tcccgaacga taagaaggca
    10201 gtttagaaag agtctctcaa aaactttaga agaatccttc tacaactcag agatccacgg
    10261 gattagtcgg atgacccaga cacctcagag ggttgggggg gtgtggcctt gctcttcaga
    10321 gagggcagat ctacttaggg agatctcttg gggaagaaaa gtggtaggca cgacagttcc
    10381 tcacccttct gagatgttgg gattacttcc caagtcctct atttcttgca cttgtggagc
    10441 aacaggagga ggcaatccta gagtttctgt atcagtactc ccgtcctttg atcagtcatt
    10501 tttttcacga ggccccctaa agggatactt gggctcgtcc acctctatgt cgacccagct
    10561 attccatgca tgggaaaaag tcactaatgt tcatgtggtg aagagagctc tatcgttaaa
    10621 agaatctata aactggttca ttactagaga ttccaacttg gctcaagctc taattaggaa
    10681 cattatgtct ctgacaggcc ctgatttccc tctagaggag gcccctgtct tcaaaaggac
    10741 ggggtcagcc ttgcataggt tcaagtctgc cagatacagc gaaggagggt attcttctgt
    10801 ctgcccgaac ctcctctctc atatttctgt tagtacagac accatgtctg atttgaccca
    10861 agacgggaag aactacgatt tcatgttcca gccattgatg ctttatgcac agacatggac
    10921 atcagagctg gtacagagag acacaaggct aagagactct acgtttcatt ggcacctccg
    10981 atgcaacagg tgtgtgagac ccattgacga cgtgaccctg gagacctctc agatcttcga
    11041 gtttccggat gtgtcgaaaa gaatatccag aatggtttct ggggctgtgc ctcacttcca
    11101 gaggcttccc gatatccgtc tgagaccagg agattttgaa tctctaagcg gtagagaaaa
    11161 gtctcaccat atcggatcag ctcaggggct cttatactca atcttagtgg caattcacga
    11221 ctcaggatac aatgatggaa ccatcttccc tgtcaacata tacggcaagg tttcccctag
    11281 agactatttg agagggctcg caaggggagt attgatagga tcctcgattt gcttcttgac
    11341 aagaatgaca aatatcaata ttaatagacc tcttgaattg gtctcagggg taatctcata
    11401 tattctcctg aggctagata accatccctc cttgtacata atgctcagag aaccgtctct
    11461 tagaggagag atattttcta tccctcagaa aatccccgcc gcttatccaa ccactatgaa
    11521 agaaggcaac agatcaatct tgtgttatct ccaacatgtg ctacgctatg agcgagagat
    11581 aatcacggcg tctccagaga atgactggct atggatcttt tcagacttta gaagtgccaa
    11641 aatgacgtac ctatccctca ttacttacca gtctcatctt ctactccaga gggttgagag
    11701 aaacctatct aagagtatga gagataacct gcgacaattg agttctttga tgaggcaggt
    11761 gctgggcggg cacggagaag ataccttaga gtcagacgac aacattcaac gactgctaaa
    11821 agactcttta cgaaggacaa gatgggtgga tcaagaggtg cgccatgcag ctagaaccat
    11881 gactggagat tacagcccca acaagaaggt gtcccgtaag gtaggatgtt cagaatgggt
    11941 ctgctctgct caacaggttg cagtctctac ctcagcaaac ccggcccctg tctcggagct
    12001 tgacataagg gccctctcta agaggttcca gaaccctttg atctcgggct tgagagtggt
    12061 tcagtgggca accggtgctc attataagct taagcctatt ctagatgatc tcaatgtttt
    12121 cccatctctc tgccttgtag ttggggacgg gtcagggggg atatcaaggg cagtcctcaa
    12181 catgtttcca gatgccaagc ttgtgttcaa cagtctttta gaggtgaatg acctgatggc
    12241 ttccggaaca catccactgc ctccttcagc aatcatgagg ggaggaaatg atatcgtctc
    12301 cagagtgata gatcttgact caatctggga aaaaccgtcc gacttgagaa acttggcaac
    12361 ctggaaatac ttccagtcag tccaaaagca ggtcaacatg tcctatgacc tcattatttg
    12421 cgatgcagaa gttactgaca ttgcatctat caaccggatc accctgttaa tgtccgattt
    12481 tgcattgtct atagatggac cactctattt ggtcttcaaa acttatggga ctatgctagt
    12541 aaatccaaac tacaaggcta ttcaacacct gtcaagagcg ttcccctcgg tcacagggtt
    12601 tatcacccaa gtaacttcgt ctttttcatc tgagctctac ctccgattct ccaaacgagg
    12661 gaagtttttc agagatgctg agtacttgac ctcttccacc cttcgagaaa tgagccttgt
    12721 gttattcaat tgtagcagcc ccaagagtga gatgcagaga gctcgttcct tgaactatca
    12781 ggatcttgtg agaggatttc ctgaagaaat catatcaaat ccttacaatg agatgatcat
    12841 aactctgatt gacagtgatg tagaatcttt tctagtccac aagatggttg atgatcttga
    12901 gttacagagg ggaactctgt ctaaagtggc tatcattata gccatcatga tagttttctc
    12961 caacagagtc ttcaacgttt ccaaacccct aactgacccc tcgttctatc caccgtctga
    13021 tcccaaaatc ctgaggcact tcaacatatg ttgcagtact atgatgtatc tatctactgc
    13081 tttaggtgac gtccctagct tcgcaagact tcacgacctg tataacagac ctataactta
    13141 ttacttcaga aagcaagtca ttcgagggaa cgtttatcta tcttggagtt ggtccaacga
    13201 cacctcagtg ttcaaaaggg tagcctgtaa ttctagcctg agtctgtcat ctcactggat
    13261 caggttgatt tacaagatag tgaagactac cagactcgtt ggcagcatca aggatctatc
    13321 cagagaagtg gaaagacacc ttcataggta caacaggtgg atcaccctag aggatatcag
    13381 atctagatca tccctactag actacagttg cctgtgaacc ggatactcct ggaagcctgc
    13441 ccatgctaag actcttgtgt gatgtatctt gaaaaaaaca agatcctaaa tctgaacctt
    13501 tggttgtttg attgtttttc tcatttttgt tgtttatttg ttaagcgtgg gtcggcatgg
    13561 catctccacc tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga
    13621 tggctaaggg aggggcgggg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
    13681 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
    13741 gggttttttg ctgaaaggag gaactatatc cggatccact agttctagag cggccgccac
    13801 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
    13861 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    13921 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    13981 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    14041 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    14101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    14161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    14221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    14281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    14341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    14401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    14461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    14521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    14581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    14641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    14701 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    14761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    14821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    14881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    14941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    15001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    15061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    15121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    15181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    15241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    15301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    15361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    15421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    15481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    15541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    15601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    15661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    15721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    15781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    15841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    15901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    15961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
