LOCUS       sequence-81436-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast marker swap plasmid for converting HIS3 to ADE2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Voth WP, Jiang YW, Stillman DJ
  TITLE     New 'marker swap' plasmids for converting selectable markers on 
            budding yeast gene disruptions and plasmids.
  JOURNAL   Yeast. 2003 Aug;20(11):985-93.
  PUBMED    12898713
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2003-08"; volume: "20(11)"; pages: "985-93"
COMMENT     Sequence Label: M2371 his3::ADE2 Disruptor Converter
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        296..483
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     698..714
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     698..714
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ggtcgactct agaggatccg ctgcacggtc ctgttcccta gcatgtacgt gagcgtattt
       61 ccttttaaac cacgacgctt tgtcttcatt caacgtttcc cattgttttt ttctactatt
      121 gctttgctgt gggaaaaact tatcgaaaga tgacgacttt ttcttaattc tcgttttaag
      181 agcttggtga gcgctaggag tcactgccag gtatcgtttg aacacggcat tagtcaggga
      241 agtcataaca cagtcctttc ccgcaatttt ctttttctat tactcttggc ctcctctagt
      301 acactctata tttttttatg cctcggtaat gattttcatt tttttttttc cacctagcgg
      361 atgactcttt ttttttctta gcgattggca ttatcacata atgaattata cattatataa
      421 agtaatgtga tttcttcgaa gaatatacta aaaaatgagc aggcaagata aacgaaggca
      481 aagatgacag agcagaaagc cctagtaaag cgtattacaa atgaaaccaa gattcagatt
      541 gcgatctctt taaagggtgg tcccctagcg atagagcact cgatcttccc agaaaaagag
      601 gcagaagcag tagcagaaca ggccacacaa tcgcaagtga ttaacgtcca cacaggtata
      661 gggtttctgg accatatgat acatgctctg gggccgctct agaactagtg gatccgggat
      721 ctcaacgttg ttactggtgt gttgaagcga tacttaagaa agctccccaa ccctatcttt
      781 acctttcaaa tatacgagcc gttgatgagg ttagttaaat ctaaaaaaat gatggaaaac
      841 ttgccatttg ttggaggaaa gttgtcctta gaagcaaaga attcggacac ttatatgtcg
      901 agcaagagcg ctttaaaaaa catattggaa gaccttccaa gggaacatta tagg
//
