LOCUS       sequence-81437-e       15606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TagRFP-T and presynaptic mGRASP component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15606)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4RFP-5premGRASP
FEATURES             Location/Qualifiers
     source          1..15606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4695..4704
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4701..5432
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             5604..5651
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             8267..8278
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13278..13296)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13292..13339
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13362..13378)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13362..13378)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13415..13435)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13415..13433)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13454..13470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13454..13470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13467..13489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13478..13494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13502..13508,13509..13526,13527..13532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13547..13568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13685..13702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13856..14444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13936..13955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14615..15406,15407..15475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15238..15257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15476..15580)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggcccgggaa agccgccacc atggtgtcta agggcgaaga gctgattaag gagaacatgc
     4741 acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg
     4801 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc
     4861 ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc agaaccttca
     4921 tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag ggcttcacat
     4981 gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc
     5041 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg
     5101 gccctgtgat gcagaagaaa acactcggct gggaggccaa caccgagatg ctgtaccccg
     5161 ctgacggcgg cctggaaggc agaaccgaca tggccctgaa gctcgtgggc gggggccacc
     5221 tgatctgcaa cttcaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc
     5281 ccggcgtcta ctatgtggac cacagactgg aaagaatcaa ggaggccgac aaagagacct
     5341 acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc
     5401 acaaacttaa tggcatggac gagctgtaca agtaatgatt aattaacgtc ctttcaacga
     5461 tccaagtcca tgaaaaaaac taacacccct cccgtacgaa agccgccacc atgccacctt
     5521 ctactagtct tttgttgctt gctgcacttc tccccttcgc tcttcccgct tcagattgga
     5581 agacaggaga agtcaccgct tcccgcgacc acatggtgct gcacgaatat gtgaacgctg
     5641 ctggcatcac aggcggtggc ggatctggcg gcggtggtag cgtcgacttt cagaaagcta
     5701 gctctattgt ttataagaag gagggggaac aggtggagtt cagtttccca ttggccttca
     5761 cagtcgagaa gctcacagga tcaggagagt tgtggtggca ggccgaaaga gcctcctcca
     5821 gcaagagttg gatcactttt gatctgaaaa acaaagaagt gagcgtgaag agagtcacac
     5881 aggaccctaa gctgcagatg ggcaagaagt tgcctttgca cttgacactc ccccaagccc
     5941 tgccccagta tgctggctcc gggaacctca ccctcgcctt ggaggctaag accggaaagt
     6001 tgcatcagga ggtcaacctc gtggtgatga gggcaacaca gctgcagaag aacctgacct
     6061 gcgaagtgtg gggtcccacc tcacctaaac tgatgctgtc tctgaagctg gagaataagg
     6121 aggctaaagt cagtaaaaga gagaaggctg tgtgggtcct taacccggag gcagggatgt
     6181 ggcagtgtct tcttagcgac tctggacagg tgctgctgga gagcaacatc aaggtgcttc
     6241 ctacttggtc aacaccagta cagccgatgg ccctgatcgt actcggggga gtcgccggcc
     6301 tgcttctgtt tatcggcctg ggcatcttct tctgcgtacg ctacaagtat cgcaacaggg
     6361 atgagggatc ttaccacgtt gacgaatcta ggaactatat cagtaacagc gctcagagta
     6421 atggcgccgt ggttaaagag aaacagccct ccagcgccaa atcagcaaac aaaaacaaga
     6481 aaaacaagga taaagagtac tacgtaagat cttaatgagc tagcttataa agtgctgggt
     6541 catctaagct tttcagtcga gaaaaaaaca ttagatcaga agaacaactg gcaacacttc
     6601 tcaacctgag acttacttca agatgctcga tcctggagag gtctatgatg accctattga
     6661 cccaatcgag ttagaggctg aacccagagg aacccccatt gtccccaaca tcttgaggaa
     6721 ctctgactac aatctcaact ctcctttgat agaagatcct gctagactaa tgttagaatg
     6781 gttaaaaaca gggaatagac cttatcggat gactctaaca gacaattgct ccaggtcttt
     6841 cagagttttg aaagattatt tcaagaaggt agatttgggt tctctcaagg tgggcggaat
     6901 ggctgcacag tcaatgattt ctctctggtt atatggtgcc cactctgaat ccaacaggag
     6961 ccggagatgt ataacagact tggcccattt ctattccaag tcgtccccca tagagaagct
     7021 gttgaatctc acgctaggaa atagagggct gagaatcccc ccagagggag tgttaagttg
     7081 ccttgagagg gttgattatg ataatgcatt tggaaggtat cttgccaaca cgtattcctc
     7141 ttacttgttc ttccatgtaa tcaccttata catgaacgcc ctagactggg atgaagaaaa
     7201 gaccatccta gcattatgga aagatttaac ctcagtggac atcgggaagg acttggtaaa
     7261 gttcaaagac caaatatggg gactgctgat cgtgacaaag gactttgttt actcccaaag
     7321 ttccaattgt ctttttgaca gaaactacac acttatgcta aaagatcttt tcttgtctcg
     7381 cttcaactcc ttaatggtct tgctctctcc cccagagccc cgatactcag atgacttgat
     7441 atctcaacta tgccagctgt acattgctgg ggatcaagtc ttgtctatgt gtggaaactc
     7501 cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg aatagtttag tccagagagc
     7561 agaaaagttt aggcctctca ttcattcctt gggagacttt cctgtattta taaaagacaa
     7621 ggtaagtcaa cttgaagaga cgttcggtcc ctgtgcaaga aggttcttta gggctctgga
     7681 tcaattcgac aacatacatg acttggtttt tgtgtttggc tgttacaggc attgggggca
     7741 cccatatata gattatcgaa agggtctgtc aaaactatat gatcaggttc accttaaaaa
     7801 aatgatagat aagtcctacc aggagtgctt agcaagcgac ctagccagga ggatccttag
     7861 atggggtttt gataagtact ccaagtggta tctggattca agattcctag cccgagacca
     7921 ccccttgact ccttatatca aaacccaaac atggccaccc aaacatattg tagacttggt
     7981 gggggataca tggcacaagc tcccgatcac gcagatcttt gagattcctg aatcaatgga
     8041 tccgtcagaa atattggatg acaaatcaca ttctttcacc agaacgagac tagcttcttg
     8101 gctgtcagaa aaccgagggg ggcctgttcc tagcgaaaaa gttattatca cggccctgtc
     8161 taagccgcct gtcaatcccc gagagtttct gaggtctata gacctcggag gattgccaga
     8221 tgaagacttg ataattggcc tcaagccaaa ggaacgggaa ttgaagattg aaggtcgatt
     8281 ctttgctcta atgtcatgga atctaagatt gtattttgtc atcactgaaa aactcttggc
     8341 caactacatc ttgccacttt ttgacgcgct gactatgaca gacaacctga acaaggtgtt
     8401 taaaaagctg atcgacaggg tcaccgggca agggcttttg gactattcaa gggtcacata
     8461 tgcatttcac ctggactatg aaaagtggaa caaccatcaa agattagagt caacagagga
     8521 tgtattttct gtcctagatc aagtgtttgg attgaagaga gtgttttcta gaacacacga
     8581 gttttttcaa aaggcctgga tctattattc agacagatca gacctcatcg ggttacggga
     8641 ggatcaaata tactgcttag atgcgtccaa cggcccaacc tgttggaatg gccaggatgg
     8701 cgggctagaa ggcttacggc agaagggctg gagtctagtc agcttattga tgatagatag
     8761 agaatctcaa atcaggaaca caagaaccaa aatactagct caaggagaca accaggtttt
     8821 atgtccgaca tacatgttgt cgccagggct atctcaagag gggctcctct atgaattgga
     8881 gagaatatca aggaatgcac tttcgatata cagagccgtc gaggaagggg catctaagct
     8941 agggctgatc atcaagaaag aagagaccat gtgtagttat gacttcctca tctatggaaa
     9001 aacccctttg tttagaggta acatattggt gcctgagtcc aaaagatggg ccagagtctc
     9061 ttgcgtctct aatgaccaaa tagtcaacct cgccaatata atgtcgacag tgtccaccaa
     9121 tgcgctaaca gtggcacaac actctcaatc tttgatcaaa ccgatgaggg attttctgct
     9181 catgtcagta caggcagtct ttcactacct gctatttagc ccaatcttaa agggaagagt
     9241 ttacaagatt ctgagcgctg aaggggagag ctttctccta gccatgtcaa ggataatcta
     9301 tctagatcct tctttgggag ggatatctgg aatgtccctc ggaagattcc atatacgaca
     9361 gttctcagac cctgtctctg aagggttatc cttctggaga gagatctggt taagctccca
     9421 agagtcctgg attcacgcgt tgtgtcaaga ggctggaaac ccagatcttg gagagagaac
     9481 actcgagagc ttcactcgcc ttctagaaga tccgaccacc ttaaatatca gaggaggggc
     9541 cagtcctacc attctactca aggatgcaat cagaaaggct ttatatgacg aggtggacaa
     9601 ggtggaaaat tcagagtttc gagaggcaat cctgttgtcc aagacccata gagataattt
     9661 tatactcttc ttaatatctg ttgagcctct gtttcctcga tttctcagtg agctattcag
     9721 ttcgtctttt ttgggaatcc ccgagtcaat cattggattg atacaaaact cccgaacgat
     9781 aagaaggcag tttagaaaga gtctctcaaa aactttagaa gaatccttct acaactcaga
     9841 gatccacggg attagtcgga tgacccagac acctcagagg gttggggggg tgtggccttg
     9901 ctcttcagag agggcagatc tacttaggga gatctcttgg ggaagaaaag tggtaggcac
     9961 gacagttcct cacccttctg agatgttggg attacttccc aagtcctcta tttcttgcac
    10021 ttgtggagca acaggaggag gcaatcctag agtttctgta tcagtactcc cgtcctttga
    10081 tcagtcattt ttttcacgag gccccctaaa gggatacttg ggctcgtcca cctctatgtc
    10141 gacccagcta ttccatgcat gggaaaaagt cactaatgtt catgtggtga agagagctct
    10201 atcgttaaaa gaatctataa actggttcat tactagagat tccaacttgg ctcaagctct
    10261 aattaggaac attatgtctc tgacaggccc tgatttccct ctagaggagg cccctgtctt
    10321 caaaaggacg gggtcagcct tgcataggtt caagtctgcc agatacagcg aaggagggta
    10381 ttcttctgtc tgcccgaacc tcctctctca tatttctgtt agtacagaca ccatgtctga
    10441 tttgacccaa gacgggaaga actacgattt catgttccag ccattgatgc tttatgcaca
    10501 gacatggaca tcagagctgg tacagagaga cacaaggcta agagactcta cgtttcattg
    10561 gcacctccga tgcaacaggt gtgtgagacc cattgacgac gtgaccctgg agacctctca
    10621 gatcttcgag tttccggatg tgtcgaaaag aatatccaga atggtttctg gggctgtgcc
    10681 tcacttccag aggcttcccg atatccgtct gagaccagga gattttgaat ctctaagcgg
    10741 tagagaaaag tctcaccata tcggatcagc tcaggggctc ttatactcaa tcttagtggc
    10801 aattcacgac tcaggataca atgatggaac catcttccct gtcaacatat acggcaaggt
    10861 ttcccctaga gactatttga gagggctcgc aaggggagta ttgataggat cctcgatttg
    10921 cttcttgaca agaatgacaa atatcaatat taatagacct cttgaattgg tctcaggggt
    10981 aatctcatat attctcctga ggctagataa ccatccctcc ttgtacataa tgctcagaga
    11041 accgtctctt agaggagaga tattttctat ccctcagaaa atccccgccg cttatccaac
    11101 cactatgaaa gaaggcaaca gatcaatctt gtgttatctc caacatgtgc tacgctatga
    11161 gcgagagata atcacggcgt ctccagagaa tgactggcta tggatctttt cagactttag
    11221 aagtgccaaa atgacgtacc tatccctcat tacttaccag tctcatcttc tactccagag
    11281 ggttgagaga aacctatcta agagtatgag agataacctg cgacaattga gttctttgat
    11341 gaggcaggtg ctgggcgggc acggagaaga taccttagag tcagacgaca acattcaacg
    11401 actgctaaaa gactctttac gaaggacaag atgggtggat caagaggtgc gccatgcagc
    11461 tagaaccatg actggagatt acagccccaa caagaaggtg tcccgtaagg taggatgttc
    11521 agaatgggtc tgctctgctc aacaggttgc agtctctacc tcagcaaacc cggcccctgt
    11581 ctcggagctt gacataaggg ccctctctaa gaggttccag aaccctttga tctcgggctt
    11641 gagagtggtt cagtgggcaa ccggtgctca ttataagctt aagcctattc tagatgatct
    11701 caatgttttc ccatctctct gccttgtagt tggggacggg tcagggggga tatcaagggc
    11761 agtcctcaac atgtttccag atgccaagct tgtgttcaac agtcttttag aggtgaatga
    11821 cctgatggct tccggaacac atccactgcc tccttcagca atcatgaggg gaggaaatga
    11881 tatcgtctcc agagtgatag atcttgactc aatctgggaa aaaccgtccg acttgagaaa
    11941 cttggcaacc tggaaatact tccagtcagt ccaaaagcag gtcaacatgt cctatgacct
    12001 cattatttgc gatgcagaag ttactgacat tgcatctatc aaccggatca ccctgttaat
    12061 gtccgatttt gcattgtcta tagatggacc actctatttg gtcttcaaaa cttatgggac
    12121 tatgctagta aatccaaact acaaggctat tcaacacctg tcaagagcgt tcccctcggt
    12181 cacagggttt atcacccaag taacttcgtc tttttcatct gagctctacc tccgattctc
    12241 caaacgaggg aagtttttca gagatgctga gtacttgacc tcttccaccc ttcgagaaat
    12301 gagccttgtg ttattcaatt gtagcagccc caagagtgag atgcagagag ctcgttcctt
    12361 gaactatcag gatcttgtga gaggatttcc tgaagaaatc atatcaaatc cttacaatga
    12421 gatgatcata actctgattg acagtgatgt agaatctttt ctagtccaca agatggttga
    12481 tgatcttgag ttacagaggg gaactctgtc taaagtggct atcattatag ccatcatgat
    12541 agttttctcc aacagagtct tcaacgtttc caaaccccta actgacccct cgttctatcc
    12601 accgtctgat cccaaaatcc tgaggcactt caacatatgt tgcagtacta tgatgtatct
    12661 atctactgct ttaggtgacg tccctagctt cgcaagactt cacgacctgt ataacagacc
    12721 tataacttat tacttcagaa agcaagtcat tcgagggaac gtttatctat cttggagttg
    12781 gtccaacgac acctcagtgt tcaaaagggt agcctgtaat tctagcctga gtctgtcatc
    12841 tcactggatc aggttgattt acaagatagt gaagactacc agactcgttg gcagcatcaa
    12901 ggatctatcc agagaagtgg aaagacacct tcataggtac aacaggtgga tcaccctaga
    12961 ggatatcaga tctagatcat ccctactaga ctacagttgc ctgtgaaccg gatactcctg
    13021 gaagcctgcc catgctaaga ctcttgtgtg atgtatcttg aaaaaaacaa gatcctaaat
    13081 ctgaaccttt ggttgtttga ttgtttttct catttttgtt gtttatttgt taagcgtggg
    13141 tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga ggacgcacgt
    13201 ccactcggat ggctaaggga ggggcgggga tccggctgct aacaaagccc gaaaggaagc
    13261 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
    13321 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatccacta gttctagagc
    13381 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
    13441 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    13501 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    13561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    13621 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    13681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    13741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    13801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    13861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    13921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    13981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    14041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    14101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    14161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    14221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    14281 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    14341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    14401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    14461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    14521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    14581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    14641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    14701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    14761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    14821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    14881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    14941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    15001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    15061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    15121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    15181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    15241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    15301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    15361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    15421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    15481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    15541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    15601 cacctg
//
