LOCUS       sequence-81438-e       15213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15213)
  TITLE     Seung Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRVdG-4tdTomato
FEATURES             Location/Qualifiers
     source          1..15213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4695..6125
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             7874..7885
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12885..12903)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12899..12946
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12969..12985)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12969..12985)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(13022..13042)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13022..13040)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13061..13077)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13061..13077)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13074..13096)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13085..13101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13109..13115,13116..13133,13134..13139))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13154..13175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13292..13309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13463..14051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13543..13562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14222..15013,15014..15082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14845..14864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15083..15187)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca
     4741 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     4801 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     4861 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     4921 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     4981 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     5041 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     5101 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     5161 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     5221 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     5281 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     5341 agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg
     5401 gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc
     5461 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     5521 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     5581 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     5641 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     5701 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     5761 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     5821 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     5881 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     5941 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     6001 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     6061 agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca
     6121 agtaagctag cttataaagt gctgggtcat ctaagctttt cagtcgagaa aaaaacatta
     6181 gatcagaaga acaactggca acacttctca acctgagact tacttcaaga tgctcgatcc
     6241 tggagaggtc tatgatgacc ctattgaccc aatcgagtta gaggctgaac ccagaggaac
     6301 ccccattgtc cccaacatct tgaggaactc tgactacaat ctcaactctc ctttgataga
     6361 agatcctgct agactaatgt tagaatggtt aaaaacaggg aatagacctt atcggatgac
     6421 tctaacagac aattgctcca ggtctttcag agttttgaaa gattatttca agaaggtaga
     6481 tttgggttct ctcaaggtgg gcggaatggc tgcacagtca atgatttctc tctggttata
     6541 tggtgcccac tctgaatcca acaggagccg gagatgtata acagacttgg cccatttcta
     6601 ttccaagtcg tcccccatag agaagctgtt gaatctcacg ctaggaaata gagggctgag
     6661 aatcccccca gagggagtgt taagttgcct tgagagggtt gattatgata atgcatttgg
     6721 aaggtatctt gccaacacgt attcctctta cttgttcttc catgtaatca ccttatacat
     6781 gaacgcccta gactgggatg aagaaaagac catcctagca ttatggaaag atttaacctc
     6841 agtggacatc gggaaggact tggtaaagtt caaagaccaa atatggggac tgctgatcgt
     6901 gacaaaggac tttgtttact cccaaagttc caattgtctt tttgacagaa actacacact
     6961 tatgctaaaa gatcttttct tgtctcgctt caactcctta atggtcttgc tctctccccc
     7021 agagccccga tactcagatg acttgatatc tcaactatgc cagctgtaca ttgctgggga
     7081 tcaagtcttg tctatgtgtg gaaactccgg ctatgaagtc atcaaaatat tggagccata
     7141 tgtcgtgaat agtttagtcc agagagcaga aaagtttagg cctctcattc attccttggg
     7201 agactttcct gtatttataa aagacaaggt aagtcaactt gaagagacgt tcggtccctg
     7261 tgcaagaagg ttctttaggg ctctggatca attcgacaac atacatgact tggtttttgt
     7321 gtttggctgt tacaggcatt gggggcaccc atatatagat tatcgaaagg gtctgtcaaa
     7381 actatatgat caggttcacc ttaaaaaaat gatagataag tcctaccagg agtgcttagc
     7441 aagcgaccta gccaggagga tccttagatg gggttttgat aagtactcca agtggtatct
     7501 ggattcaaga ttcctagccc gagaccaccc cttgactcct tatatcaaaa cccaaacatg
     7561 gccacccaaa catattgtag acttggtggg ggatacatgg cacaagctcc cgatcacgca
     7621 gatctttgag attcctgaat caatggatcc gtcagaaata ttggatgaca aatcacattc
     7681 tttcaccaga acgagactag cttcttggct gtcagaaaac cgaggggggc ctgttcctag
     7741 cgaaaaagtt attatcacgg ccctgtctaa gccgcctgtc aatccccgag agtttctgag
     7801 gtctatagac ctcggaggat tgccagatga agacttgata attggcctca agccaaagga
     7861 acgggaattg aagattgaag gtcgattctt tgctctaatg tcatggaatc taagattgta
     7921 ttttgtcatc actgaaaaac tcttggccaa ctacatcttg ccactttttg acgcgctgac
     7981 tatgacagac aacctgaaca aggtgtttaa aaagctgatc gacagggtca ccgggcaagg
     8041 gcttttggac tattcaaggg tcacatatgc atttcacctg gactatgaaa agtggaacaa
     8101 ccatcaaaga ttagagtcaa cagaggatgt attttctgtc ctagatcaag tgtttggatt
     8161 gaagagagtg ttttctagaa cacacgagtt ttttcaaaag gcctggatct attattcaga
     8221 cagatcagac ctcatcgggt tacgggagga tcaaatatac tgcttagatg cgtccaacgg
     8281 cccaacctgt tggaatggcc aggatggcgg gctagaaggc ttacggcaga agggctggag
     8341 tctagtcagc ttattgatga tagatagaga atctcaaatc aggaacacaa gaaccaaaat
     8401 actagctcaa ggagacaacc aggttttatg tccgacatac atgttgtcgc cagggctatc
     8461 tcaagagggg ctcctctatg aattggagag aatatcaagg aatgcacttt cgatatacag
     8521 agccgtcgag gaaggggcat ctaagctagg gctgatcatc aagaaagaag agaccatgtg
     8581 tagttatgac ttcctcatct atggaaaaac ccctttgttt agaggtaaca tattggtgcc
     8641 tgagtccaaa agatgggcca gagtctcttg cgtctctaat gaccaaatag tcaacctcgc
     8701 caatataatg tcgacagtgt ccaccaatgc gctaacagtg gcacaacact ctcaatcttt
     8761 gatcaaaccg atgagggatt ttctgctcat gtcagtacag gcagtctttc actacctgct
     8821 atttagccca atcttaaagg gaagagttta caagattctg agcgctgaag gggagagctt
     8881 tctcctagcc atgtcaagga taatctatct agatccttct ttgggaggga tatctggaat
     8941 gtccctcgga agattccata tacgacagtt ctcagaccct gtctctgaag ggttatcctt
     9001 ctggagagag atctggttaa gctcccaaga gtcctggatt cacgcgttgt gtcaagaggc
     9061 tggaaaccca gatcttggag agagaacact cgagagcttc actcgccttc tagaagatcc
     9121 gaccacctta aatatcagag gaggggccag tcctaccatt ctactcaagg atgcaatcag
     9181 aaaggcttta tatgacgagg tggacaaggt ggaaaattca gagtttcgag aggcaatcct
     9241 gttgtccaag acccatagag ataattttat actcttctta atatctgttg agcctctgtt
     9301 tcctcgattt ctcagtgagc tattcagttc gtcttttttg ggaatccccg agtcaatcat
     9361 tggattgata caaaactccc gaacgataag aaggcagttt agaaagagtc tctcaaaaac
     9421 tttagaagaa tccttctaca actcagagat ccacgggatt agtcggatga cccagacacc
     9481 tcagagggtt gggggggtgt ggccttgctc ttcagagagg gcagatctac ttagggagat
     9541 ctcttgggga agaaaagtgg taggcacgac agttcctcac ccttctgaga tgttgggatt
     9601 acttcccaag tcctctattt cttgcacttg tggagcaaca ggaggaggca atcctagagt
     9661 ttctgtatca gtactcccgt cctttgatca gtcatttttt tcacgaggcc ccctaaaggg
     9721 atacttgggc tcgtccacct ctatgtcgac ccagctattc catgcatggg aaaaagtcac
     9781 taatgttcat gtggtgaaga gagctctatc gttaaaagaa tctataaact ggttcattac
     9841 tagagattcc aacttggctc aagctctaat taggaacatt atgtctctga caggccctga
     9901 tttccctcta gaggaggccc ctgtcttcaa aaggacgggg tcagccttgc ataggttcaa
     9961 gtctgccaga tacagcgaag gagggtattc ttctgtctgc ccgaacctcc tctctcatat
    10021 ttctgttagt acagacacca tgtctgattt gacccaagac gggaagaact acgatttcat
    10081 gttccagcca ttgatgcttt atgcacagac atggacatca gagctggtac agagagacac
    10141 aaggctaaga gactctacgt ttcattggca cctccgatgc aacaggtgtg tgagacccat
    10201 tgacgacgtg accctggaga cctctcagat cttcgagttt ccggatgtgt cgaaaagaat
    10261 atccagaatg gtttctgggg ctgtgcctca cttccagagg cttcccgata tccgtctgag
    10321 accaggagat tttgaatctc taagcggtag agaaaagtct caccatatcg gatcagctca
    10381 ggggctctta tactcaatct tagtggcaat tcacgactca ggatacaatg atggaaccat
    10441 cttccctgtc aacatatacg gcaaggtttc ccctagagac tatttgagag ggctcgcaag
    10501 gggagtattg ataggatcct cgatttgctt cttgacaaga atgacaaata tcaatattaa
    10561 tagacctctt gaattggtct caggggtaat ctcatatatt ctcctgaggc tagataacca
    10621 tccctccttg tacataatgc tcagagaacc gtctcttaga ggagagatat tttctatccc
    10681 tcagaaaatc cccgccgctt atccaaccac tatgaaagaa ggcaacagat caatcttgtg
    10741 ttatctccaa catgtgctac gctatgagcg agagataatc acggcgtctc cagagaatga
    10801 ctggctatgg atcttttcag actttagaag tgccaaaatg acgtacctat ccctcattac
    10861 ttaccagtct catcttctac tccagagggt tgagagaaac ctatctaaga gtatgagaga
    10921 taacctgcga caattgagtt ctttgatgag gcaggtgctg ggcgggcacg gagaagatac
    10981 cttagagtca gacgacaaca ttcaacgact gctaaaagac tctttacgaa ggacaagatg
    11041 ggtggatcaa gaggtgcgcc atgcagctag aaccatgact ggagattaca gccccaacaa
    11101 gaaggtgtcc cgtaaggtag gatgttcaga atgggtctgc tctgctcaac aggttgcagt
    11161 ctctacctca gcaaacccgg cccctgtctc ggagcttgac ataagggccc tctctaagag
    11221 gttccagaac cctttgatct cgggcttgag agtggttcag tgggcaaccg gtgctcatta
    11281 taagcttaag cctattctag atgatctcaa tgttttccca tctctctgcc ttgtagttgg
    11341 ggacgggtca ggggggatat caagggcagt cctcaacatg tttccagatg ccaagcttgt
    11401 gttcaacagt cttttagagg tgaatgacct gatggcttcc ggaacacatc cactgcctcc
    11461 ttcagcaatc atgaggggag gaaatgatat cgtctccaga gtgatagatc ttgactcaat
    11521 ctgggaaaaa ccgtccgact tgagaaactt ggcaacctgg aaatacttcc agtcagtcca
    11581 aaagcaggtc aacatgtcct atgacctcat tatttgcgat gcagaagtta ctgacattgc
    11641 atctatcaac cggatcaccc tgttaatgtc cgattttgca ttgtctatag atggaccact
    11701 ctatttggtc ttcaaaactt atgggactat gctagtaaat ccaaactaca aggctattca
    11761 acacctgtca agagcgttcc cctcggtcac agggtttatc acccaagtaa cttcgtcttt
    11821 ttcatctgag ctctacctcc gattctccaa acgagggaag tttttcagag atgctgagta
    11881 cttgacctct tccacccttc gagaaatgag ccttgtgtta ttcaattgta gcagccccaa
    11941 gagtgagatg cagagagctc gttccttgaa ctatcaggat cttgtgagag gatttcctga
    12001 agaaatcata tcaaatcctt acaatgagat gatcataact ctgattgaca gtgatgtaga
    12061 atcttttcta gtccacaaga tggttgatga tcttgagtta cagaggggaa ctctgtctaa
    12121 agtggctatc attatagcca tcatgatagt tttctccaac agagtcttca acgtttccaa
    12181 acccctaact gacccctcgt tctatccacc gtctgatccc aaaatcctga ggcacttcaa
    12241 catatgttgc agtactatga tgtatctatc tactgcttta ggtgacgtcc ctagcttcgc
    12301 aagacttcac gacctgtata acagacctat aacttattac ttcagaaagc aagtcattcg
    12361 agggaacgtt tatctatctt ggagttggtc caacgacacc tcagtgttca aaagggtagc
    12421 ctgtaattct agcctgagtc tgtcatctca ctggatcagg ttgatttaca agatagtgaa
    12481 gactaccaga ctcgttggca gcatcaagga tctatccaga gaagtggaaa gacaccttca
    12541 taggtacaac aggtggatca ccctagagga tatcagatct agatcatccc tactagacta
    12601 cagttgcctg tgaaccggat actcctggaa gcctgcccat gctaagactc ttgtgtgatg
    12661 tatcttgaaa aaaacaagat cctaaatctg aacctttggt tgtttgattg tttttctcat
    12721 ttttgttgtt tatttgttaa gcgtgggtcg gcatggcatc tccacctcct cgcggtccga
    12781 cctgggcatc cgaaggagga cgcacgtcca ctcggatggc taagggaggg gcggggatcc
    12841 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
    12901 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
    12961 tatatccgga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
    13021 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
    13081 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    13141 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    13201 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    13261 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    13321 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    13381 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    13441 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    13501 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    13561 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    13621 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    13681 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    13741 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    13801 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    13861 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    13921 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    13981 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    14041 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    14101 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    14161 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    14221 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    14281 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    14341 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    14401 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    14461 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    14521 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    14581 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    14641 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    14701 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    14761 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    14821 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    14881 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    14941 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    15001 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    15061 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    15121 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    15181 ttccgcgcac atttccccga aaagtgccac ctg
//
