LOCUS       sequence-81469-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Wu CJ, Conze DB, Li T, Srinivasula SM, Ashwell JD
  TITLE     NEMO is a sensor of Lys 63-linked polyubiquitination and functions 
            in NF-kappaB activation.
  JOURNAL   Nat Cell Biol. 2006 Apr . 8(4):398-406.
  PUBMED    16547522
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Apr . 8(4):398-406."
COMMENT     Sequence Label: pCMVTAG-NEMOL329P
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgat tacgccaagc tcgaaattaa
       61 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgcc accatggatt
      121 acaaggatga cgacgataag agcccgggcg gatcccccgg gctgcaggaa ttcaataggc
      181 acctctggaa gagccaactg tgtgagatgg tgcagcccag tggtggcccg gcagcagatc
      241 aggacgtact gggcgaagag tctcctctgg ggaagccagc catgctgcac ctgccttcag
      301 aacagggcgc tcctgagacc ctccagcgct gcctggagga gaatcaagag ctccgagatg
      361 ccatccggca gagcaaccag attctgcggg agcgctgcga ggagcttctg catttccaag
      421 ccagccagag ggaggagaag gagttcctca tgtgcaagtt ccaggaggcc aggaaactgg
      481 tggagagact cggcctggag aagctcgatc tgaagaggca gaaggagcag gctctgcggg
      541 aggtggagca cctgaagaga tgccagcagc agatggctga ggacaaggcc tctgtgaaag
      601 cccaggtgac gtccttgctc ggggagctgc aggagagcca gagtcgcttg gaggctgcca
      661 ctaaggaatg ccaggctctg gagggtcggg cccgggcggc cagcgagcag gcgcggcagc
      721 tggagagtga gcgcgaggcg ctgcagcagc agcacagcgt gcaggtggac cagctgcgca
      781 tgcagggcca gagcgtggag accgcgctcc gcatggagcg ccaggccgcc tcggaggaga
      841 agaggaagct ggcccagttg caggtggcct atcaccagct cttccaagaa tacgacaacc
      901 acatcaagag cagcgtggtg ggcagtgagc ggaagcgaga a
//
