LOCUS       sequence-81475-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI
  TITLE     HES1 inhibits cycling of hematopoietic progenitor cells via DNA 
            binding.
  JOURNAL   Stem Cells. 2006 Apr . 24(4):876-88.
  PUBMED    16513761
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2006 Apr . 24(4):876-88."
COMMENT     Sequence Label: EF.hICN1.CMV.GFP
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(98..100,101..103,104..817)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(98..100,101..103,104..814)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(143..164)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(404..423)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     751..772
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag
       61 ctcaagcttc gaattggggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct
      121 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      181 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      241 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      301 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      361 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      421 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      481 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      541 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
      601 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      661 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
      721 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      781 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgtcg acgggcccgc
      841 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
      901 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
      961 tgctttttgc ttgtactggg tctctctggt tagacca
//
