LOCUS       sequence-81477-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Magnifico A, Ettenberg S, Yang C, Mariano J, Tiwari S, Fang S, 
            Lipkowitz S, Weissman AM
  TITLE     WW domain HECT E3s target Cbl RING finger E3s for proteasomal 
            degradation.
  JOURNAL   J Biol Chem. 2003 Oct 31. 278(44):43169-77.
  PUBMED    12907674
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Oct 31. 278(44):43169-77."
COMMENT     Sequence Label: pCINeo-myc-Itch/2859
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          170..302
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     285..304
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        347..365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      397..406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             406..435
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
       61 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      121 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      181 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      241 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
      301 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
      361 ataggctagc gtttaaactt aagcttggta ccagccgcca ccatggagca gaagctgatt
      421 tccgaggagg acctgaacgg tggtagtctg accatgaaat ctcaacttca gatcactgtc
      481 atctcagcca aacttaaaga aaataaaaag aattggtttg gaccaagtcc ttatgtagaa
      541 gtcacagtag atggacagtc aaagaagaca gaaaaatgca ataatacaaa cagtcccaag
      601 tggaagcagc ccctcacagt tattgttacc cctacgagta aattatgttt tcgtgtgtgg
      661 agtcaccaga ccctgaagtc tgatgtttta ttgggaactg ctggattaga tatttatgaa
      721 acattaaagt caaacaatat gaaacttgaa gaagtagtta tgactttgca gcttgtaggt
      781 gacaaagagc caacagagac gatgggagat ttgtcagttt gtcttgatgg gctgcaagta
      841 gaagctgagg ttgttactaa cggtgaaacg tcatgctccg agagtactac acagaatgat
      901 gatggctgca gaaccagaga tgatacaaga gtgagcacaa atggatcaga ggaccctgag
      961 gttgcagcgt caggggaaaa caagagggcc aatgggaaca attctccgtc cctttcaaat
     1021 ggtggtttta agccttctag acctcctaga cct
//
