LOCUS       sequence-81483-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing MSCV LTR promoter, dCas9 that is 
            fused to 2x NLS, VP64 and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pMSCV-LTR-dCas9-VP64-BFP
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatctcgcc accatggaca agaagtattc
       61 tatcggactg gccatcggga ctaatagcgt cgggtgggcc gtgatcactg acgagtacaa
      121 ggtgccctct aagaagttca aggtgctcgg gaacaccgac cggcattcca tcaagaaaaa
      181 tctgatcgga gctctcctct ttgattcagg ggagaccgct gaagcaaccc gcctcaagcg
      241 gactgctaga cggcggtaca ccaggaggaa gaaccggatt tgttaccttc aagagatatt
      301 ctccaacgaa atggcaaagg tcgacgacag cttcttccat aggctggaag aatcattcct
      361 cgtggaagag gataagaagc atgaacggca tcccatcttc ggtaatatcg tcgacgaggt
      421 ggcctatcac gagaaatacc caaccatcta ccatcttcgc aaaaagctgg tggactcaac
      481 cgacaaggca gacctccggc ttatctacct ggccctggcc cacatgatca agttcagagg
      541 ccacttcctg atcgagggcg acctcaatcc tgacaatagc gatgtggata aactgttcat
      601 ccagctggtg cagacttaca accagctctt tgaagagaac cccatcaatg caagcggagt
      661 cgatgccaag gccattctgt cagcccggct gtcaaagagc cgcagacttg agaatcttat
      721 cgctcagctg ccgggtgaaa agaaaaatgg actgttcggg aacctgattg ctctttcact
      781 tgggctgact cccaatttca agtctaattt cgacctggca gaggatgcca agctgcaact
      841 gtccaaggac acctatgatg acgatctcga caacctcctg gcccagatcg gtgaccaata
      901 cgccgacctt ttccttgctg ctaagaatct ttctgacgcc atcctgcctg tctgacattc
      961 tccgcgtgaa cactgaaa
//
