LOCUS       sequence-81484-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing MSCV LTR promoter, dCas9 that is 
            fused to 2x NLS, VP64 and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pMSCV-LTR-dCas9-VP64-BFP
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaggaagccc gcattcctgt caggcgaaca aaagaaggca attgtggacc ttctgttcaa
       61 gaccaataga aaggtgaccg tgaagcagct gaaggaggac tatttcaaga aaattgaatg
      121 cttcgactct gtggagatta gcggggtcga agatcggttc aacgcaagcc tgggtaccta
      181 ccatgatctg cttaagatca tcaaggacaa ggattttctg gacaatgagg agaaagagga
      241 catccttgag gacattgtcc tgactctcac tctgttcgag gaccgggaaa tgatcgagga
      301 gaggcttaag acctacgccc atctgttcga cgataaagtg atgaagcaac ttaaacggag
      361 aagatatacc ggatggggac gccttagccg caaactcatc aacggaatcc gggacaaaca
      421 gagcggaaag accattcttg atttccttaa gagcgacgga ttcgctaatc gcaacttcat
      481 gcaacttatc catgatgatt ccctgacctt taaggaggac atccagaagg cccaagtgtc
      541 tggacaaggt gactcactgc acgagcatat cgcaaatctg gctggttcac ccgctattaa
      601 gaagggtatt ctccagaccg tgaaagtcgt ggacgagctg gtcaaggtga tgggtcgcca
      661 taaaccagag aacattgtca tcgagatggc cagggaaaac cagactaccc agaagggaca
      721 gaagaacagc agggagcgga tgaaaaga
//
