LOCUS       sequence-81486-e       15047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a myc-tagged version of hCas9 in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 15047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pRB14
FEATURES             Location/Qualifiers
     source          1..15047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..202,203..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    759..792
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     primer_bind     complement(812..831)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     931..953
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(991..1009)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1077..1181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1182..1250,1251..2042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1400..1419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2213..2801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2702..2721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(3036..3066,3067..3621)
                     /label=P element 5' end
     gene            complement(3632..7760)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     7733..7751
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             10406..10435
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             10436..14536
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             10436..14536
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             10436..14536
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             14549..14569
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgcgtactcg caaattatta aaaataaaac tttaaaaata atttcgtcta attaatatta
       61 tgagttaatt caaaccccac ggacatgcta agggttaatc aacaatcata tcgctgtctc
      121 actcagactc aatacgacac tcagaatact attcctttca ctcgcactta ttgcaagcat
      181 acgttaagtg gatgtctctt gccgacggga ccaccttatg ttatttcatc atggtctggc
      241 cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg
      301 caaggagtag ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac
      361 catcgtgttt actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata
      421 aaaacaagat atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga
      481 ctttggattt gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc
      541 gtttatcgct acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc
      601 gccctcttca taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc
      661 cacccgctct ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag
      721 ttccgcgcga ttcggtgcgg tatttcacac cgcatatacg cgcccgggga gcccaagggc
      781 acgccctggc acccatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      841 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
      901 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      961 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     1021 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1081 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1141 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1201 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1261 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1321 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1381 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1441 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1501 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1561 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1621 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1681 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1741 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1801 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1861 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1921 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1981 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2041 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2101 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2161 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2221 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2281 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2341 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2401 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2461 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2521 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2581 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
     2641 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2701 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2761 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccttc ttcttgaact
     2821 cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca
     2881 acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat
     2941 cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg
     3001 ccgccgatct gccgctggac tacgtgggtc tggcccatga tgaaataaca taaggtggtc
     3061 ccgtcgatag ccgaagctta ccgaagtata cacttaaatt cagtgcacgt ttgcttgttg
     3121 agaggaaagg ttgtgtgcgg acgaattttt ttttgaaaac attaaccctt acgtggaata
     3181 aaaaaaaatg aaatattgca aattttgctg caaagctgtg actggagtaa aattaattca
     3241 cgtgccgaag tgtgctatta agagaaaatt gtgggagcag agccttgggt gcagccttgg
     3301 tgaaaactcc caaatttgtg atacccactt taaagattcg cagtggaagg ctgcacctgc
     3361 aaaaggtcag acatttaaaa ggaggcgact caacgcagat gccgtaccta gtaaagtgat
     3421 agagcctgaa ccagaaaaga taaaagaagg ctataccagt gggagtacac aaacagagta
     3481 agtttgaata gtaaaaaaaa tcatttatgt aaacaataac gtgactgtgc gttaggtcct
     3541 gttcattgtt taatgaaaat aagagcttga gggaaaaaat tcgtactttg gagtacgaaa
     3601 tgcgtcgttt agagcagcag ccgaattaat tctagttcca gtgaaatcca agcattttct
     3661 aaattaaatg tattcttatt attatagttg ttatttttga tatatataaa caacactatt
     3721 atgcccacca tttttttgag atgcatctac acaaggaaca aacactggat gtcactttca
     3781 gttcaaattg taacgctaat cactccgaac aggtcacaaa aaattacctt aaaaagtcat
     3841 aatattaaat tagaataaat atagctgtga gggaaatata tacaaatata ttggagcaaa
     3901 taaattgtac atacaaatat ttattactaa tttctattga gacgaaatga accactcgga
     3961 accatttgag cgaaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca
     4021 aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg
     4081 gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat
     4141 atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta
     4201 agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata
     4261 cttgtattct ataggtcata tcttgttttt attggcacaa atataattac attagctttt
     4321 tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa acaagcagtt
     4381 atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag ccagatatgc
     4441 gagcacccgg aagctcacga tgagaatggc cagacccacg tagtccagcg gcagatcggc
     4501 ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga
     4561 cgatgtgcag ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc
     4621 gttggcgtaa cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc
     4681 cgagttcaag aagaagccgc caaagagcag gaatggtatg ataaccggcg gacccacaga
     4741 cagcgccatc gaggtcgagg agctggcgca ggatattaga tatccgaagg acgttgacac
     4801 attggccacc agagtgacca gcgccaggca gttgaagaag tgcagcactc cggcccgcag
     4861 tccgatcatc ggataggcaa tcgccgtgaa gaccagtggc actgtgagaa aaagcggtaa
     4921 ttcggcaatc gttttgccca gaaagtatgt gtcacagcga taaagtcgac ttcgggcctc
     4981 cctcataaaa actggcagct ctgaggtgaa cacctaaatc gaatcgattc attagaaagt
     5041 tagtaaatta ttaatatgca aatgtattct aaacaagact tacatttatc gtggcaaaga
     5101 cgttttgaaa ggtcatgttg gtcaggaaga ggaagatggc tccgttgata ttcatcacgc
     5161 ccacttgcgt gagttgttgg cccaaaaaga tgaggccaat caagatggca accatctgca
     5221 aattaaaatg ttactcgcat ctcattaata ttcatatctt caacatgttc gcgagttaaa
     5281 tgaaatttat ttattttctg caaaactata aactatacat ctcattgaaa aaaactaaga
     5341 agggtgtgga atcaggcaat tctaactaaa atctagcgaa tttgtttcca agaattgtaa
     5401 gcgttatatc atttgtttcc actggaacca ctcaccgttg tctgaataag tcgcactttt
     5461 acgaggagtg gttccttgag caccgacagc caggatcgcc acaggaccgc ccggaactgc
     5521 atgaaccagg tggccttgta ggtgtaccca ttctccggct gctccagtgg cttctccaaa
     5581 tttttggtgg ccaacaactg ctccatatcc cgggctactt tgctaatagc aaaattgtcg
     5641 ccatatcttg gcgatccgat cacgggactc gatctcccgt ccgggcacaa cggccaacac
     5701 ctgtacgtaa aagtccgccg gattgtagtt ggtaggacac tgggcaccca cgctggatag
     5761 gagttgagat gtaatgtaat gctagatacc cttaataaac acatcgaact cactaggaaa
     5821 agaagtcgac ggcttcgctg ggagtgccca agaaagctac cctgccctcg gccatcagaa
     5881 ggatcttgtc aaagagctca aacagctcgg aagacggctg atgaatggtc aggatgacgg
     5941 tcttgccctt ctgcgacagc ttcttcagca cctggacgac gctgtgggcg gtaaatgagt
     6001 ccagtccgga ggtgggctca tcgcagatca gaagcggcgg atcggttagt gcctcggagg
     6061 cgaatgccag acgcttcctt tctccgccgg acagaccttt caccctgccg ggcacaccga
     6121 tgatcgtgtg ctgacatttg ctgagcgaaa gctcctggat cacctgatcc acgcgggcca
     6181 ctcgctgccg ataggtcaga tgtcgtggca tccgcaccat ggcctggaaa atcaggtgtt
     6241 ccctggccgt tagggagccg ataaagaggt catcctgctg gacataggcg cacctggcct
     6301 gcatctcctt ggcgtccaca ggttggccat tgagcagtcg catcccggat ggcgatactt
     6361 ggatgccctg cggcgatcga aaggcaaggg cattcagcag ggtcgtcttt ccggcaccgg
     6421 aactgcccat cacggccaaa agttcgcccg gataggccac gccgcaaact gagtttcaaa
     6481 ttggtaattg gaccctttat taagatttca cacagatcag ccgactgcga atagaaactc
     6541 accgttcttg agcaaatgtt tcctgggcgc cggtatgtgt cgctcgttgc agaatagtcc
     6601 gcgtgtccgg ttgaccagct gccgccatcc ggagcccggc tgattgaccg ccccaaagat
     6661 gtccatattg tgccaggcat aggtgaggtt ctcggctagt tggccgctcc ctgaaccgga
     6721 gtcctccggc ggactgggtg gcaggagcgt gccgtagttt ttggcctgcc cgaagccctg
     6781 gttaatgcag ctctgcgaag cgtccgctgt caccctgcaa tgatagggga tctcaaatat
     6841 caactacaag cgttatgctc atctaacccc gaacaaaacg aagtatccta cgaagtaggt
     6901 ttatactttt atttattttt tgtgcatcta ggatcagctt aaaatatctg gttgttatat
     6961 tttttgtaaa aaagaatgta gtcgaaaatg aatgccttta gatgtcttga tcatgatatg
     7021 atcttaaaaa ttgtcttata tagcgagcac agctaccaga ataatctgtt tcgtgtcact
     7081 atttgtttgt gcgattgcgg tttgggattt ttgtgggtcg cagttctcac gccgcagaca
     7141 atttgatgtt gcaatcgcag ttcctataga tcaagtgaac ttaagatgta tgcacatgta
     7201 ctactcacat tgttcagatg ctcggcagat gggtgtttgc tgcctccgcg aattaatagc
     7261 tcctgatcct cttggcccat tgccgggatt tttcacactt tcccctgctt acccacccaa
     7321 aaaccaatca ccaccccaat cactcaaaaa acaaacaaaa ataagaagcg agaggagttt
     7381 tggcacagca ctttgtgttt aattgatggc gtaaaccgct tggagcttcg tcacgaaacc
     7441 gctgacaaaa tgcaactgaa ggcggacatt gacgctacgt aacgctacaa acggtggcga
     7501 aagagatagc ggacgcagcg gcgaaagaga cggcgatatt tctgtggaca gagaaggagg
     7561 caaacagcgc tgactttgag tggaatgtca ttttgagtga gaggtaatcg aaagaacctg
     7621 gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg atcagataag ttcaatgata
     7681 tccagtgcag taaaaaaaaa aaatgttttt tttatctact ttccgcaaaa atgggtttta
     7741 ttaacttaca tacatactag aattgggccg gccttaatta agtggaattc gatatcaagc
     7801 ttgcacaggt cctgttcgat aacgtcgtac tcgggaatca cctttaactt gtccgctatt
     7861 gggtatttgt ccttaaacac attcgtaatc tctcccacaa tcattgccct ggcctctgca
     7921 atgtaaaaag tatttataaa atatcgctgg ctctaaagaa aacatattaa tcaaagtatt
     7981 gaggctttat caatctaaca tttacaagta gtcagaccac aatttttaga ctgcacttct
     8041 gcgtaagtaa atgaaatgaa atggtgacct actcttgggt agccgtactg ttatatgctg
     8101 acgagccttc acctgggcct gctttttctc ctgtttcgtc tgatgcgtcg gatgcggctg
     8161 gactggctga ttgttgggat tgggttttgg atgctgctcc gaaaggtggg ccacagcttt
     8221 tgcgatggtg gtgcccacta tgcggttgct gcaggaacta cagctgaatg ttcgctttat
     8281 tgtctgcagc tcctggggct ggacgaaggc cacgccctca cccggtgcgg ccatctcctg
     8341 cggcaaaacg tatgtgggat tcctgtccaa gtagctctcg atgcccacca taatgcgctc
     8401 cagcaggttg gccaggtgcg ggtctagctg cggcggattc gtcgtctggt tcactgagtt
     8461 tagggtctgc agaaagaagt gtgcctccat ggtaagcgga ttcatctgca cgatcagcgg
     8521 gctttagtct aaaaattcga atgatacaaa ctcgtactca cgtaaagcag tttgtttggc
     8581 atcatctcgg ccatcgtctt attcttcttc gtggcctttg ttaccggagc ggctatgttg
     8641 ggcgcggtgg agcccggaag ctgaaaggtt aatcgaatgc gttaactttt ctgcaactcg
     8701 aaagtttgcc gcctttgttc gactgccaat aactgttgat tcgaaaattc gaatcgaagc
     8761 ggttgaattt cgtagggtgg ccaactacac caaagttcgc cggcggtgta tgcatgtgag
     8821 tgtgagtgtg acagtgggtg tacgtgtgct ttaagcgaca caatcgcacc gcgatgtaca
     8881 tatttttgta gaaatgtgtg tgcatttttc gtccggatca cggctccata aaataaatca
     8941 tatcacaggg ctcagggatt tgtgagtaga agttcccgcc acttaaacgt cacttgtgaa
     9001 atcgccatcg acggtcaact tttactcaca ctggtcttcc gcggacggac cgtctcaaag
     9061 tactgccttt ctgcgttgga aaacatcgcc tttttcgtcc aaaaggagtc cccaggttcg
     9121 atccgcatgg cgttgtgcgt gcgtgccttt cttttcaaat gattacggct attaacttgg
     9181 gggcgttaag ttggaaacac gtaaattgca gactgcgatt agagtgacca tgagtaggag
     9241 ttcaaaatct cctgacatca ttttcttaaa acctgctttg ttttttacat ttctatttaa
     9301 tataactcct atttgaataa aaaaacaaaa caagtttaga tgttaagata ttaactacat
     9361 cctttgctcc aaagggagag gggaagttat ggagttaatt aatttgctgt tggaaatcaa
     9421 tatggagtca gaaatataat gatttactaa accttattga atcggtaacg atgcgaattt
     9481 atattaaaat agcttttatg aaacattcaa caaaaatatt attaatgttg gcccacttta
     9541 gcaaccggtt aggtctaccg gttgggcaag caaagattca cgccctggtt cgagtcccaa
     9601 ctagtcctgc aaaataccgc agcaagtttt agagagacca agtgccatta cctctcccac
     9661 ttcagttatc ggttatgcgg cgtttaagtc gacagcttgc cgtctctagc tccggtgcct
     9721 atataaagca gcccgctttc cacatttcat attcgtttta cgtttgtcaa gcctcatagc
     9781 cggcagttcg aacgtatacg ctctctgagt cagacctcga aatcgtagct ctacacaatt
     9841 ctgtgaattt tccttgtcgc gtgtgaaaca cttccaataa aaactcaata tggtgagtac
     9901 tttaaaaaaa aatctagtga aataatgctg aaaagaaatt tgtgtgggca aaattcaatg
     9961 ggcaaaaacg cgatgcggct ttttctcaaa atggcggccg gcctgcgttt tttcctcaaa
    10021 agtgatgacg tcatgcctgt tttttttttt tgttcgcaat gaggaatggc tcttaaaatc
    10081 tagataaaaa aaatattcat tatttctatg ctgctggaac gcttcattaa tcttaaaaat
    10141 tctaaattcg gttaccatga tacttcgacg cataactgta gattttggat agaattaaag
    10201 agaaaatggc gagagagtaa aattccggcg tcggcaaagt agagcaaaaa aatcagtata
    10261 ccatttagct acctctctca ctcgcacgca gtgccggctc aagttgggcg cggctctgca
    10321 attatcgatt ttcttggggt gtgtaactaa tcatccgttt tcccttcctc ctcatccaca
    10381 gcgtgaaggt acctaatcca aaatggaaca gaaactgatt agcgaagaag acctggacaa
    10441 gaagtactcc attgggctcg atatcggcac aaacagcgtc ggctgggccg tcattacgga
    10501 cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat
    10561 aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg
    10621 gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca
    10681 ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga
    10741 gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt
    10801 ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt
    10861 agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa
    10921 atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa
    10981 actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc
    11041 atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga
    11101 aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc
    11161 cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa
    11221 gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg
    11281 cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag
    11341 tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa
    11401 gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact
    11461 gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat
    11521 tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat
    11581 ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg
    11641 cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct
    11701 caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat
    11761 cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc
    11821 gtggatgact cgcaaatcag aagagaccat cactccctgg aacttcgagg aagtcgtgga
    11881 taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc
    11941 taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga
    12001 gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga
    12061 gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca
    12121 gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt
    12181 ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga
    12241 caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct
    12301 tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt
    12361 cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc
    12421 aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct
    12481 taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg actctctcac
    12541 ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca
    12601 catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt
    12661 cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat
    12721 ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag
    12781 gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa
    12841 cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta
    12901 cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggatc atatcgtgcc
    12961 ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa
    13021 tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg
    13081 gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc
    13141 tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga
    13201 gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta
    13261 cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt
    13321 ctcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca
    13381 tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa
    13441 gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc
    13501 aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat
    13561 gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat
    13621 cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt
    13681 ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg
    13741 aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa
    13801 aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt
    13861 actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact
    13921 gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga
    13981 ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct
    14041 ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg
    14101 taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga
    14161 aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa
    14221 acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc
    14281 cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag
    14341 ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc
    14401 cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct
    14461 ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc
    14521 tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtgt gagcggccgc
    14581 ataggccact agtggatctg gatccttctt aagaagagct ctaaagcttt atctagagtc
    14641 gacctcgaac gttaactcga ggcctataag atctcttgca tgctacctgc aggataccgg
    14701 tataacgcgt atagctagca ttcgaatcgg aaatcaatcg aattctcgat caatgttttg
    14761 cgaaaaaaaa tgaaacgaga acaaatgaat tcagttgtaa aagatttgtt ctcttaacaa
    14821 cgtaatcaat aaagtcggat ccaatttgtt cacaagtctc ctagcattcg aatcggaaat
    14881 caatcgaatt ctcgatcaat gttttgcgaa aaaaaatgaa acgagaacaa atgaattcag
    14941 ttgtaaaaga tttgttctct taacaacgta atcaataaag tcggatccaa ttttgtcaca
    15001 agtctcctag gtctgcgatc gcaaaggcgc gccatgcagc caagctt
//
