LOCUS       sequence-81491-e       11335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses FLP recombinase in Drosophila .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 11335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pMH5
FEATURES             Location/Qualifiers
     source          1..11335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2060..3331
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     polyA_signal    3453..3534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3490..3509)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3544..3563
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     gene            3576..7704
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(3585..3603)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4055..4126,4521..4794,4869..5523,5585..5900,6121..6252,
                     6323..6937)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(7715..8269,8270..8300)
                     /label=P element 5' end
     rep_origin      complement(8535..9123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8615..8634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9294..10085,10086..10154))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9917..9936
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10155..10259)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10327..10345
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10383..10405)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10505..10524
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    10545..10578
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     misc_feature    join(11103..11133,11134..11335)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttgggc tgcaggtcga ctctagctag aggatgtcgc cggaacgcag cgacagggat
       61 tccaatgtgt ccgtatcctt caggcttttg cccttcagtt ccaggcgcag cgactggcga
      121 ttcgcgtgtg gggtctgctt cagggtcttg tggattaggg cgcgcagatc gccgatgggc
      181 gtggcgccgg agggcacctt caccttgccg tacggcttgc tgttcttcgc gttcaaaatc
      241 tccagctcca ttttgctttc ggtgcgcttg caatcagtac tgtccaaaat cgaaaatcgc
      301 cgaaccgtag tgtgaccgtg cggggctctg cgaaaataaa cttttttagg tatatggcca
      361 cacacgggga aagcacagtg gattatatgt attaatatat tatgcaggtt ttcattactt
      421 atccagatgt aagcccactt aaagcgattt aacaattatt tgccgaaaga gtataaacaa
      481 atttcacata aaaatggatt aagaaaagct tgtgtaagat tatgcgcagc gttgccagat
      541 agctccattt aaaacacttc aaaaacaata agtttagaaa atatatacat aaatagcagt
      601 cgttgccgca acgctcaaca catcacactt ttaaaacacc ctttacctac acagaattac
      661 tttttaaatt tccagtcaag ctgcgagttt caaaattata gccggtagag aagacagtgc
      721 tatttcaaaa gcaaactaac aagggtctta aatttcaaaa caccaatcct aacaagcctt
      781 ggacttttgt aagtttagat caaaggtggc attgcattca atgtcatggt aagaagtagg
      841 tcgtctaggt agaaatcctc attcagccgg tcaagtcagt acgagaaagg tctcaatttg
      901 aaattgtctt aaaaatattt tattgttttg tactgtggtg agtttaaacg aaaaacacaa
      961 aaaaaaagtg atacacagaa atcataaaaa attttaatac aaggtattcg tacgtatcaa
     1021 aaacatttcg gcacaatttt ttttctctgt actaaagtgt tacgaacact acggtatttt
     1081 ttagtgattt tcaacggaca ccgaaggtat ataaacagcg ttcgcgaacg gtcgccttca
     1141 aaaccaattg acatttgcag cagcaagtac aagtagaaag taaagcgcaa tcagcgaaaa
     1201 atttatactt aattgttggt gattaaagta caattaaaag aacattctcg aaagtcacaa
     1261 gaaacgtaag tttttaactc gctgttacca attagtaata agagcaacaa gacgttgagt
     1321 aatttcaaga aaaactgcat ttcaaggtct ttgttcggcc attttttttt tattcaacgc
     1381 tctacgtaat tacaaaataa gaaattggca gccacgcatc ttgttttccc aatgaattgg
     1441 catcaaaacg caaacaaatc tataaataaa acttgcgtgt tgattttcgc caagatttat
     1501 tggcaaattg tgaaattcgc agtgacgcat ttgaaaattc gagaaatcac gaacgcactc
     1561 gagcatttgt gtgcatgtta ttagttagtt gtttagttaa ttgaagtatt taccaacgaa
     1621 atccacttat ttttagctga aatagagtag gttgcttaaa caaagccacg tctgaaaatt
     1681 tcttattgct tgtagttgtg acgtcaccat atacacacaa aataatgtgt atgcatgcat
     1741 ttcagctgtg tatatataca tgcacacact cgcaacacga aaacgatgac gaagcaacgg
     1801 aacaaaggtt tctcaactac cctttgttcc ctgtttcttc gctttccttt gttccaatat
     1861 tcgtagaggg ttaatagggg tttctcaaca aagttggcgt cgataaataa gtttcccatt
     1921 tttattcccc agccagaagt tagtttcaat agttttgtaa tttcaacgaa actcatttga
     1981 tttcgtacta attttccaca tctctatttt ctgcccgcag aataatccaa actgcaggtc
     2041 gactctagct agaggatcca tgccacaatt tggtatatta tgtaaaacac cacctaaggt
     2101 gcttgttcgt cagtttgtgg aaaggtttga aagaccttca ggtgagaaaa tagcattatg
     2161 tgctgctgaa ctaacctatt tatgttggat gattacacat aacggaacag caatcaagag
     2221 agccacattc atgagctata atactatcat aagcaattcg ctgagtttcg atattgtcaa
     2281 taaatcactc cagtttaaat acaagacgca aaaagcaaca attctggaag cctcattaaa
     2341 gaaattgatt cctgcttggg aatttacaat tattccttac tatggacaaa aacatcaatc
     2401 tgatatcact gatattgtaa gtagtttgca attacagttc gaatcatcgg aagaagcaga
     2461 taagggaaat agccacagta aaaaaatgct taaagcactt ctaagtgagg gtgaaagcat
     2521 ctgggagatc actgagaaaa tactaaattc gtttgagtat acttcgagat ttacaaaaac
     2581 aaaaacttta taccaattcc tcttcctagc tactttcatc aattgtggaa gattcagcga
     2641 tattaagaac gttgatccga aatcatttaa attagtccaa aataagtatc tgggagtaat
     2701 aatccagtgt ttagtgacag agacaaagac aagcgttagt aggcacatat acttctttag
     2761 cgcaaggggt aggatcgatc cacttgtata tttggatgaa tttttgagga attctgaacc
     2821 agtcctaaaa cgagtaaata ggaccggcaa ttcttcaagc aataaacagg aataccaatt
     2881 attaaaagat aacttagtca gatcgtacaa taaagctttg aagaaaaatg cgccttattc
     2941 aatctttgct ataaaaaatg gcccaaaatc tcacattgga agacatttga tgacctcatt
     3001 tctttcaatg aagggcctaa cggagttgac taatgttgtg ggaaattgga gcgataagcg
     3061 tgcttctgcc gtggccagga caacgtatac tcatcagata acagcaatac ctgatcacta
     3121 cttcgcacta gtttctcggt actatgcata tgatccaata tcaaaggaaa tgatagcatt
     3181 gaaggatgag actaatccaa ttgaggagtg gcagcatata gaacagctaa agggtagtgc
     3241 tgaaggaagc atacgatacc ccgcatggaa tgggataata tcacaggagg tactagacta
     3301 cctttcatcc tacataaata gacgcatata agcggccgcg actctagatc ataatcagcc
     3361 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     3421 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     3481 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3541 gttgtggttt gtccaaactc atcaatgtat gaattctagt atgtatgtaa gttaataaaa
     3601 cccatttttg cggaaagtag ataaaaaaaa catttttttt ttttactgca ctggatatca
     3661 ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa gggtatttga tgtaccaggt
     3721 tctttcgatt acctctcact caaaatgaca ttccactcaa agtcagcgct gtttgcctcc
     3781 ttctctgtcc acagaaatat cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc
     3841 accgtttgta gcgttacgta gcgtcaatgt ccgccttcag ttgcattttg tcagcggttt
     3901 cgtgacgaag ctccaagcgg tttacgccat caattaaaca caaagtgctg tgccaaaact
     3961 cctctcgctt cttatttttg tttgtttttt gagtgattgg ggtggtgatt ggttttgggt
     4021 gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt
     4081 aattcgcgga ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg
     4141 tgcatacatc ttaagttcac ttgatctata ggaactgcga ttgcaacatc aaattgtctg
     4201 cggcgtgaga actgcgaccc acaaaaatcc caaaccgcaa tcgcacaaac aaatagtgac
     4261 acgaaacaga ttattctggt agctgtgctc gctatataag acaattttta agatcatatc
     4321 atgatcaaga catctaaagg cattcatttt cgactacatt cttttttaca aaaaatataa
     4381 caaccagata ttttaagctg atcctagatg cacaaaaaat aaataaaagt ataaacctac
     4441 ttcgtaggat acttcgtttt gttcggggtt agatgagcat aacgcttgta gttgatattt
     4501 gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg
     4561 gcttcgggca ggccaaaaac tacggcacgc tcctgccacc cagtccgccg gaggactccg
     4621 gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct
     4681 ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac
     4741 tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt
     4801 ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg
     4861 aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg
     4921 tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt
     4981 atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc
     5041 caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca
     5101 cctgattttc caggccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc
     5161 ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg
     5221 tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc
     5281 cgaggcacta accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc
     5341 atttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt
     5401 catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct
     5461 gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc
     5521 ctagtgagtt cgatgtgttt attaagggta tctagcatta cattacatct caactcctat
     5581 ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt
     5641 tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca
     5701 attttgctat tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatttgg
     5761 agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt
     5821 tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag
     5881 tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca
     5941 attcttggaa acaaattcgc tagattttag ttagaattgc ctgattccac acccttctta
     6001 gtttttttca atgagatgta tagtttatag ttttgcagaa aataaataaa tttcatttaa
     6061 ctcgcgaaca tgttgaagat atgaatatta atgagatgcg agtaacattt taatttgcag
     6121 atggttgcca tcttgattgg cctcatcttt ttgggccaac aactcacgca agtgggcgtg
     6181 atgaatatca acggagccat cttcctcttc ctgaccaaca tgacctttca aaacgtcttt
     6241 gccacgataa atgtaagtct tgtttagaat acatttgcat attaataatt tactaacttt
     6301 ctaatgaatc gattcgattt aggtgttcac ctcagagctg ccagttttta tgagggaggc
     6361 ccgaagtcga ctttatcgct gtgacacata ctttctgggc aaaacgattg ccgaattacc
     6421 gctttttctc acagtgccac tggtcttcac ggcgattgcc tatccgatga tcggactgcg
     6481 ggccggagtg ctgcacttct tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc
     6541 aacgtccttc ggatatctaa tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt
     6601 gggtccgccg gttatcatac cattcctgct ctttggcggc ttcttcttga actcgggctc
     6661 ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg
     6721 tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa
     6781 caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga
     6841 tctgccgctg gactacgtgg gtctggccat tctcatcgtg agcttccggg tgctcgcata
     6901 tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg
     6961 cttgtttttt tttttaccat tattaccatc gtgtttactg tttattgccc cctcaaaaag
     7021 ctaatgtaat tatatttgtg ccaataaaaa caagatatga cctatagaat acaagtattt
     7081 ccccttcgaa catccccaca agtagacttt ggatttgtct tctaaccaaa agacttacac
     7141 acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt
     7201 ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt
     7261 ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc
     7321 gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga
     7381 gtggttcatt tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc
     7441 tccaatatat ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac
     7501 tttttaaggt aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa
     7561 gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag
     7621 tgttgtttat atatatcaaa aataacaact ataataataa gaatacattt aatttagaaa
     7681 atgcttggat ttcactggaa ctagaattaa ttcggctgct gctctaaacg acgcatttcg
     7741 tactccaaag tacgaatttt ttccctcaag ctcttatttt cattaaacaa tgaacaggac
     7801 ctaacgcaca gtcacgttat tgtttacata aatgattttt tttactattc aaacttactc
     7861 tgtttgtgta ctcccactgg tatagccttc ttttatcttt tctggttcag gctctatcac
     7921 tttactaggt acggcatctg cgttgagtcg cctcctttta aatgtctgac cttttgcagg
     7981 tgcagccttc cactgcgaat ctttaaagtg ggtatcacaa atttgggagt tttcaccaag
     8041 gctgcaccca aggctctgct cccacaattt tctcttaata gcacacttcg gcacgtgaat
     8101 taattttact ccagtcacag ctttgcagca aaatttgcaa tatttcattt ttttttattc
     8161 cacgtaaggg ttaatgtttt caaaaaaaaa ttcgtccgca cacaaccttt cctctcaaca
     8221 agcaaacgtg cactgaattt aagtgtatac ttcggtaagc ttcggctatc gacgggacca
     8281 ccttatgtta tttcatcatg ggccagaccc acgtagtcca gcggcagatc ggcggcggag
     8341 aagttaagcg tctccaggat gaccttgccc gaactggggc acgtggtgtt cgacgatgtg
     8401 cagctaattt cgcccggctc cacgtccgcc cattggttaa tcagcagacc ctcgttggcg
     8461 taacggaacc atgagaggta cgacaaccat ttgaggtata ctggcaccga gcccgagttc
     8521 aagaagaagg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8581 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8641 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8701 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     8761 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8821 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8881 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8941 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9001 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9061 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9121 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9181 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9241 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9301 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9361 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9421 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9481 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9541 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9601 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9661 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9721 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9781 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9841 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9901 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9961 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10021 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10081 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10141 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10201 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10261 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    10321 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
    10381 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    10441 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
    10501 tatgcggcat cagagcagat tgtactgaga gtgcaccata tggggtgcca gggcgtgccc
    10561 ttgggctccc cgggcgcgta tatgcggtgt gaaataccgc accgaatcgc gcggaactaa
    10621 cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag cgggtgggag
    10681 acagcgaaag agcaactacg aaacgtggtg tggtggaggt gaattatgaa gagggcgcgc
    10741 gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg ataaacgagt
    10801 ttttgatgta aggtatgcag gtgtgtaagt cttttggtta gaagacaaat ccaaagtcta
    10861 cttgtgggga tgttcgaagg ggaaatactt gtattctata ggtcatatct tgtttttatt
    10921 ggcacaaata taattacatt agctttttga gggggcaata aacagtaaac acgatggtaa
    10981 taatggtaaa aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg
    11041 ggcccgaagt cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag
    11101 accatgatga aataacataa ggtggtcccg tcggcaagag acatccactt aacgtatgct
    11161 tgcaataagt gcgagtgaaa ggaatagtat tctgagtgtc gtattgagtc tgagtgagac
    11221 agcgatatga ttgttgatta acccttagca tgtccgtggg gtttgaatta actcataata
    11281 ttaattagac gaaattattt ttaaagtttt atttttaata atttgcgagt acgca
//
