LOCUS       sequence-81492-e       11539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Double-GFP reporter for DNA cleavage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, 
            Philippou-Massier J, Wieland I, Gaul U, Forstemann K
  TITLE     Efficient chromosomal gene modification with CRISPR/cas9 and 
            PCR-based homologous recombination donors in cultured Drosophila 
            cells.
  JOURNAL   Nucleic Acids Res. 2014 Apr 19.
  PUBMED    24748663
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Apr 19."
COMMENT     Sequence Label: pKF251
FEATURES             Location/Qualifiers
     source          1..11539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2070..2079
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2079..2108
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(2112..2114,2115..2117,2118..2831)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2112..2114,2115..2117,2118..2828)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2865..2867,2868..3581)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2865..2867,2868..3578)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    3699..3780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3736..3755)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3790..3809
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     gene            3822..7950
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(3831..3849)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4301..4372,4767..5040,5115..5769,5831..6146,6367..6498,
                     6569..7183)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(7961..8515,8516..8546)
                     /label=P element 5' end
     rep_origin      complement(8781..9369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8861..8880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9540..10331,10332..10400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10163..10182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10401..10505)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10573..10591
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10629..10651)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10751..10770
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(11307..11337,11338..11539)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 aagcttgggc tgcaggtcga ctctagctag aggatgtcgc cggaacgcag cgacagagat
       61 tccaatgtgt ccgtatcttt caggcttttg cccttcagtt ccagacgaag cgactggcga
      121 ttcgcgtgtg gggtctgctt cagggtcttg tgaattaggg cgcgcagatc gccgatgggc
      181 gtggcgccgg agggcacctt caccttgccg tacggcttgc tgttcttcgc gttcaaaatc
      241 tccagctcca ttttgctttc ggtgcgcttg caatcagtac tgtccaaaat cgaaaatcgc
      301 cgaaccgtag tgtgaccgtg cggggctctg cgaaaataaa cttttttagg tatatggcca
      361 cacacgggga aagcacagtg gattatatgt tttaatatta taatatgcag gttttcatta
      421 cttatccaga tgtaagccca cttaaagcga tttaacaatt atttgccgaa agagtataaa
      481 caaatttcac ataaaaatgg attaagaaaa gcttgtgtaa gattatgcgc agcgttgcca
      541 gatagctcca tttaaaacac ttcaaaaaca ataagtttag aaaatatata cataaatagc
      601 agtcgttgcc gcaacgctca acacatcaca cttttaaaac accctttacc tacacagaat
      661 tactttttaa atttccagtc aagctgcgag tttcaaaatt atagccggta gagaagacag
      721 tgctatttca aaagcaaact aacaagggtc ttaaatttca aaacaccaat cctaacaagc
      781 cttggacttt tgtaagttta gatcaaaggt ggcattgcat tcaatgtcat ggtaagaagt
      841 aggtcgtcta ggtagaaatc ctcattcagc cggtcaagtc agtacgagaa aggtctcaat
      901 ttgaaattgt cttaaaaata ttttattgtt ttgtactgtg gtgagtttaa acgaaaaaca
      961 caaaaaaaaa gtgatacaca gaaatcataa aaaattttaa tacaaggtat tcgtacgtat
     1021 caaaaacatt tcggcacaat tttttttctc tgtactaaag tgttacgaac actacggtat
     1081 tttttagtga ttttcaacgg acaccgaagg tatataaaca gcgttcgcga acggtcgcct
     1141 tcaaaaccaa ttgacatttg cagcagcaag tacaagtaga aagtaaagcg caatcagcga
     1201 aaaatttata cttaattgtt ggtgattaaa gtacaattaa aagaacattc tcgaaagtca
     1261 caagaaacgt aagtttttaa ctcgctgtta ccaattagta ataagagcaa caagacgttg
     1321 agtaatttca agaaaaactg catttcaagg tctttgttcg gccatttttt ttttattcaa
     1381 cgctctacgt aattacaaaa taagaaattg gcagccacgc atcttgtttt cccaatgaat
     1441 tggcatcaaa acgcaaacaa atctataaat aaaacttgcg tgttgatttt cgccaagatt
     1501 tattggcaaa ttgtgaaatt cgcagtgacg catttgaaaa ttcgagaaat cacgaacgca
     1561 ctcgagcatt tgtgtgcatg ttattagtta gttgtttagt taattgaagt atttaccaac
     1621 gaaatccact tatttttagc tgaaatagag taggttgctt aaacaaagcc acgtctgaaa
     1681 atttcttatt gcttgtagtt gtgacgtcac catatacaca caaaataatg tgtatgcatg
     1741 catttcagct gtgtatatat acatgcacac actcgcaaca cgaaaacgat gacgaagcaa
     1801 cggaacaaag gtttctcaac taccctttgt tccctgtttc ttcgctttcc tttgttccaa
     1861 tattcgtaga gggttaatag gggtttctca acaaagttgg cgtcgataaa taagtttccc
     1921 atttttattc cccagccaga agttagtttc aatagttttg taatttcaac gaaactcatt
     1981 tgatttcgta ctaattttcc acatctctat tttctgcccg cagaataatc caaactgcag
     2041 gtcgactcta gctagaggat ccaccggtcg ccaccatgga acaaaaactt atttctgaag
     2101 aagacttggg catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2161 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2221 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2281 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     2341 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2401 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2461 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2521 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     2581 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     2641 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     2701 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     2761 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2821 tgtacaagta aagcggccta gggataacag ggtaatgcgg ccgcgtgagc aagggcgagg
     2881 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2941 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3001 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3061 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3121 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3181 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3241 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3301 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     3361 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3421 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     3481 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3541 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggcctc tagatcataa
     3601 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     3661 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     3721 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3781 attctagttg tggtttgtcc aaactcatca atgtatgaat tctagtatgt atgtaagtta
     3841 ataaaaccca tttttgcgga aagtagataa aaaaaacatt tttttttttt actgcactgg
     3901 atatcattga acttatctga tcagttttaa atttacttcg atccaagggt atttgatgta
     3961 ccaggttctt tcgattacct ctcactcaaa atgacattcc actcaaagtc agcgctgttt
     4021 gcctccttct ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct
     4081 ttcgccaccg tttgtagcgt tacgtagcgt caatgtccgc cttcagttgc attttgtcag
     4141 cggtttcgtg acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc
     4201 aaaactcctc tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt
     4261 ttgggtgggt aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga
     4321 gctattaatt cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag
     4381 tacatgtgca tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat
     4441 tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa ccgcaatcgc acaaacaaat
     4501 agtgacacga aacagattat tctggtagct gtgctcgcta tataagacaa tttttaagat
     4561 catatcatga tcaagacatc taaaggcatt cattttcgac tacattcttt tttacaaaaa
     4621 atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa
     4681 acctacttcg taggatactt cgttttgttc ggggttagat gagcataacg cttgtagttg
     4741 atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta
     4801 accagggctt cgggcaggcc aaaaactacg gcacgctcct gccacccagt ccgccggagg
     4861 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     4921 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     4981 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     5041 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     5101 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     5161 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     5221 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     5281 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     5341 ggaacacctg attttccagg ccatggtgcg gatgccacga catctgacct atcggcagcg
     5401 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     5461 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     5521 cgcctccgag gcactaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     5581 ggactcattt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     5641 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     5701 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     5761 cttttcctag tgagttcgat gtgtttatta agggtatcta gcattacatt acatctcaac
     5821 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
     5881 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
     5941 gcgacaattt tgctattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa
     6001 atttggagaa gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca
     6061 tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg
     6121 taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg
     6181 cttacaattc ttggaaacaa attcgctaga ttttagttag aattgcctga ttccacaccc
     6241 ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaaaata aataaatttc
     6301 atttaactcg cgaacatgtt gaagatatga atattaatga gatgcgagta acattttaat
     6361 ttgcagatgg ttgccatctt gattggcctc atctttttgg gccaacaact cacgcaagtg
     6421 ggcgtgatga atatcaacgg agccatcttc ctcttcctga ccaacatgac ctttcaaaac
     6481 gtctttgcca cgataaatgt aagtcttgtt tagaatacat ttgcatatta ataatttact
     6541 aactttctaa tgaatcgatt cgatttaggt gttcacctca gagctgccag tttttatgag
     6601 ggaggcccga agtcgacttt atcgctgtga cacatacttt ctgggcaaaa cgattgccga
     6661 attaccgctt tttctcacag tgccactggt cttcacggcg attgcctatc cgatgatcgg
     6721 actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc tggtggccaa
     6781 tgtgtcaacg tccttcggat atctaatatc ctgcgccagc tcctcgacct cgatggcgct
     6841 gtctgtgggt ccgccggtta tcataccatt cctgctcttt ggcggcttct tcttgaactc
     6901 gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa
     6961 cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta gctgcacatc
     7021 gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta acttctccgc
     7081 cgccgatctg ccgctggact acgtgggtct ggccattctc atcgtgagct tccgggtgct
     7141 cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa
     7201 taactgcttg tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc
     7261 aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa
     7321 gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac
     7381 ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata
     7441 tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca
     7501 ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt
     7561 ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc gctcaaatgg
     7621 ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta tgtacaattt
     7681 atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct aatttaatat
     7741 tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt acaatttgaa
     7801 ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa atggtgggca
     7861 taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat acatttaatt
     7921 tagaaaatgc ttggatttca ctggaactag aattaattcg gctgctgctc taaacgacgc
     7981 atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt aaacaatgaa
     8041 caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta ctattcaaac
     8101 ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg gttcaggctc
     8161 tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg tctgaccttt
     8221 tgcaggtgca gccttccact gcgaatcttt aaagtgggta tcacaaattt gggagttttc
     8281 accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac acttcggcac
     8341 gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt tcattttttt
     8401 ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca acctttcctc
     8461 tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg gctatcgacg
     8521 ggaccacctt atgttatttc atcatgggcc agacccacgt agtccagcgg cagatcggcg
     8581 gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt ggtgttcgac
     8641 gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag cagaccctcg
     8701 ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg caccgagccc
     8761 gagttcaaga agaaggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8821 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8881 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8941 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     9001 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9061 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9121 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9181 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9241 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9301 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9361 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9421 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9481 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9541 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9601 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9661 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9721 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9781 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9841 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9901 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9961 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10021 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10081 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10141 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10201 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10261 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10321 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10381 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10441 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10501 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    10561 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
    10621 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    10681 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    10741 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    10801 ccgcaccgaa tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa
    10861 tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg
    10921 aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg
    10981 tgttttatgt agcgataaac gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg
    11041 gttagaagac aaatccaaag tctacttgtg gggatgttcg aaggggaaat acttgtattc
    11101 tataggtcat atcttgtttt tattggcaca aatataatta cattagcttt ttgagggggc
    11161 aataaacagt aaacacgatg gtaataatgg taaaaaaaaa aacaagcagt tatttcggat
    11221 atatgtcggc tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg
    11281 gaagctcacg atgagaatgg ccagaccatg atgaaataac ataaggtggt cccgtcggca
    11341 agagacatcc acttaacgta tgcttgcaat aagtgcgagt gaaaggaata gtattctgag
    11401 tgtcgtattg agtctgagtg agacagcgat atgattgttg attaaccctt agcatgtccg
    11461 tggggtttga attaactcat aatattaatt agacgaaatt atttttaaag ttttattttt
    11521 aataatttgc gagtacgca
//
