LOCUS       sequence-81510-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Link AJ, Phillips D, Church GM
  TITLE     Methods for generating precise deletions and insertions in the 
            genome of wild-type Escherichia coli: application to open reading 
            frame characterization.
  JOURNAL   J Bacteriol. 1997 Oct . 179(20):6228-37.
  PUBMED    9335267
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 1997 Oct . 179(20):6228-37."
COMMENT     Sequence Label: pKOV
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(575..592)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa gggccatcgc tcaacggata
       61 aaaggtactc cggggataac aggctgatac cgcccaagag ttcatatcga cggcggtgtt
      121 tggcacctcg atgtcggctc atcacatcct ggagctgaag taggtcccaa gggtatggct
      181 gttcgccatt taaagtggta cgcgagctgg gtttagaacg tcgtgagaca gttcggtccc
      241 tatctgccgt gggcgctgga gaactgaggg gggctgctcc tagtacgaga ggaccggagt
      301 ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac tgcccggtag ctaaatgcgg
      361 aagagataag tgctgaaagc atctaagcac gaaacttgcc ccgagatgag ttctccctga
      421 ctccttgaga gtcctgaagg aacgttgaag acgacgacgt tgataggccg ggtgtgtaag
      481 cgcagcgatg cgttgagcta accggtacta atgaaccgtg aggcttaacc ttacaacgcc
      541 gaagatgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
      601 gaagcggtct gatgaaacag aatttgcctg gcggccgtag cgcggtggtc ccacctgacc
      661 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
      721 cgagagtagg gaactgccag gcatcaaatt aagcagtaag ccggtcataa aaccggtggt
      781 tgtaaaagaa ttcggtggag cggtagttca gtcggttaga atacctgcct gtcacgcagg
      841 gggtcgcggg ttcgagtccc gtccgttccg ccaccctaat taggggcgta gttcaattgg
      901 tag
//
