LOCUS       sequence-81512-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Link AJ, Phillips D, Church GM
  TITLE     Methods for generating precise deletions and insertions in the 
            genome of wild-type Escherichia coli: application to open reading 
            frame characterization.
  JOURNAL   J Bacteriol. 1997 Oct . 179(20):6228-37.
  PUBMED    9335267
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 1997 Oct . 179(20):6228-37."
COMMENT     Sequence Label: pKOV
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      596..975
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(682..701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     892..913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcatccgcca aaacatcttc ggcgttgtaa ggttaagcct cacggttcat tagtaccggt
       61 tagctcaacg catcgctgcg cttacacacc cggcctatca acgtcgtcgt cttcaacgtt
      121 ccttcaggac tctcaaggag tcagggagaa ctcatctcgg ggcaagtttc gtgcttagat
      181 gctttcagca cttatctctt ccgcatttag ctaccgggca gtgccattgg catgacaacc
      241 cgaacaccag tgatgcgtcc actccggtcc tctcgtacta ggagcagccc ccctcagttc
      301 tccagcgccc acggcagata gggaccgaac tgtctcacga cgttctaaac ccagctcgcg
      361 taccacttta aatggcgaac agccataccc ttgggaccta cttcagctcc aggatgtgat
      421 gagccgacat cgaggtgcca aacaccgccg tcgatatgaa ctcttgggcg gtatcagcct
      481 gttatccccg gagtaccttt tatccgttga gcgatggccc ttccattcag aaccaccgga
      541 tcactatgac ctgctttcgc acctgggatc ctctagagtc gaccggagaa tggcgcgcgc
      601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      961 ttttgccgat ttcggcctat tggttaaaaa a
//
