LOCUS       sequence-81516-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Link AJ, Phillips D, Church GM
  TITLE     Methods for generating precise deletions and insertions in the 
            genome of wild-type Escherichia coli: application to open reading 
            frame characterization.
  JOURNAL   J Bacteriol. 1997 Oct . 179(20):6228-37.
  PUBMED    9335267
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 1997 Oct . 179(20):6228-37."
COMMENT     Sequence Label: pKOV
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgatgaact gctgtacatt ttgatacgtt tttccgtcac cgtcaaagat tgatttataa
       61 tcctctacac cgttgatgtt caaagagctg tctgatgctg atacgttaac ttgtgcagtt
      121 gtcagtgttt gtttgccgta atgtttaccg gagaaatcag tgtagaataa acggattttt
      181 ccgtcagatg taactgtggc tgaacctgac cattcttgtg tttggtcttt taggatagaa
      241 tcatttgcat cgaatttgtc gctgtcttta aagacgcggc cagcgttttt ccagctgtca
      301 atagaagttt cgccgacttt ttgatagaac atgtaaatcg atgtgtcatc cgcattttta
      361 ggatctccgg ctaatgcaaa gacgatgtgg tagccgtgat agtttgcgac agtgccgtca
      421 gcgttttgta atggccagct gtcccaaacg tccaggcctt ttgcagaaga gatattttta
      481 attgtggacg aatcgaactc aggaacttga tatttttcat ttttttgctg ttcagggatt
      541 tgcagcatat catggcgtgt aatatgggaa atgccgtatg tttccttata tggcttttgg
      601 ttcgtttctt tcgcaaacgc ttgagttgcg cctcctgcca gcagtgcggt agtaaaggtt
      661 aatactgttg cttgttttgc aaactttttg atgttcatcg ttcatgtctc cttttttatg
      721 tactgtgtta gcggtctgct tcttccagcc ctcctgtttg aagatggcaa gttagttacg
      781 cacaataaaa aaagacctaa aatatgtaag gggtgacgcc aaagtataca ctttgccctt
      841 tacacatttt aggtcttgcc tgctttatca gtaacaaacc cgcgcgattt acttttcgac
      901 ctcattctat tagactctcg tttggattgc aactggtcta ttttcctctt ttgtttgata
      961 gaaaatcata aaaggatttg cagactacgg gcctaaagaa ctaa
//
