LOCUS       sequence-81522-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express DsRed monomer-tagged hAIM2 in mammalian cells  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pMSCVpuro-hAIM2-DsRed monomer
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..679)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(262..281)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      676..685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctactggga gccggagtgg cgggcctcgg cgtgctcgta ctgctccacc acggtgtagt
       61 cctcgttgtg gttggtgatg tccagcttgg agtccacgta gtggttgccg ggcagctgca
      121 cgggcttctt ggccttgtac acggtcttga agtcgcaggt gtagtggccg ccgtccttca
      181 gcttcagggc gtgggagatc tcgcccttca gcacgccgtc ctgggggtac agcttctcgg
      241 tggagggctc ccagccggca gtcttcttct gcattacggg gccgtcggcg gggaagttca
      301 cgcccttgaa cttcaccttg tagatgaagg tgccgtcctg cagggaggag tcctgctgca
      361 cctccaccac gccgccgtcc tcgaagttca tggagcgctc ccaggtgaag ccctcgggga
      421 aggacagctt catgtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
      481 actggaactg gggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct
      541 gcagcttggc ggtctgggtg ccctcgtagg gcttgccctc gccctcgccc tcgatctcga
      601 agtagtggcc gttcacggag ccctccatgc gcaccttgaa ctgcatgaac tccttgatga
      661 cgtcctcggt gttgtccatg gtggcgaccg gtacccgggg atcctctgtt ttttttttgg
      721 ccttaataac ctttatggtg ctatgaactc cagatgtcag ctgtagtttt tctccatttt
      781 ttgacagtgt gaagaatgta agtcgaacct tatctccttc c
//
