LOCUS       sequence-81523-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: MSCV-N-Flag-HA-IRES-PURO
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(102..108,109..126,127..132))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(157..281)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(297..323)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(336..359)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      359..368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(381..797)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(397..413)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(429..451)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
       61 agctcctgaa aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc
      121 ataaagtgta aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt
      181 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
      241 tcattttacg tttctcgttc agcttttttg tacaaacttg tttgataacc tcgagcagcg
      301 taatctggaa catcgtatgg gtatcctcca tcgagcttgt catcgtcatc cttgtagtcc
      361 atggtggcag atctaattcc ggcgcctaga gaaggagtga gggctggata aagggaggat
      421 cgaggcgggg tcgaacgagg aggttcaagg gggagagacg gggcggatgg aggaagagga
      481 ggcggaggct tagggtgtac aaagggcttg acccagggag gggggtcaaa agccaaggct
      541 tcccaggtca cgatgtaggg gacctggtct gggtgtccat gcgggccagg tgaaaagacc
      601 ttgatcttaa cctgggtgat gaggcctcgg ttaaaggtgc cgtctcgcgg ccatccgacg
      661 ttaaaggttg gccattctgc agagcagaag gtaacccaac gtctcttctt gacatctacc
      721 gactggttgt gagcgatccg ctcgacatct ttccagtgac ctaaggtcaa acttaaggga
      781 gtggtaacag tctggcccta attttcagac aaatacagaa acacagtcag acagagacaa
      841 cacagaacga tgctgcagcg ctgcagcaga caagacgcgc ggcttcggtt ccaaaccgaa
      901 agcaaaaatt cagacggagg cgggaactgt tttaggttct cgtctcctac cagaaccaca
      961 tatcctgacg gggtcggatt cca
//
