LOCUS       sequence-81535-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: MSCV-N-Flag-HA-IRES-PURO
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    269..836
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    286..838
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(430..447)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     657..676
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(850..869)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 tcttgtacaa agtggttcga taacgaattc cgcccccccc ccctaacgtt actggccgaa
      301 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
      361 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
      421 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
      481 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
      541 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
      601 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
      661 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
      721 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
      781 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
      841 gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
      901 agggccgtac gcaccctcgc cgccgcgttc gccga
//
