LOCUS       sequence-81545-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMV driven mammalian expression of ligase-dead UBR5 with an 
            N-termianl GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grofte M, 
            Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B,
            Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C
  TITLE     TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at 
            damaged chromosomes.
  JOURNAL   Cell. 2012 Aug 17;150(4):697-709. doi: 10.1016/j.cell.2012.06.039. 
            Epub 2012 Aug 9.
  PUBMED    22884692
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.06.039"; journalName: "Cell"; date: 
            "2012-08-17- 17"; volume: "150"; issue: "4"; pages: "697-709"
COMMENT     Sequence Label: GFP-UBR5 ∆HECT
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..55
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gacgagctgt acaagggctc gagcccatca acaagtttgt acaaaaaagc aggcttccgg
       61 accatgacgt ccatccattt cgtggttcac ccgctgccgg gcaccgagga ccagctcaat
      121 gacaggttac gagaagtttc tgagaagctg aacaaatata atttaaacag ccacccccct
      181 ttgaatgtat tggaacaggc tactattaaa cagtgtgtgg tgggaccaaa tcatgctgcc
      241 tttcttcttg aggatggtag agtttgcagg attggttttt cagtacagcc agacagattg
      301 gaattgggta aacctgataa taatgatggg tcaaagttga acagcaactc gggggcaggg
      361 aggacgtcaa ggcctggtag gacaagcgac tctccatggt ttctctcagg ttctgagact
      421 ctaggcaggc tggcaggcaa caccttagga agccgctgga gttctggagt gggtggaagt
      481 ggtggaggat cctctggtag gtcatcagct ggagctcgag attcccgccg gcagactcga
      541 gttattcgga caggacggga tcgagggtct gggcttttgg gcagtcagcc ccagccagtt
      601 attccagcat ctgtcattcc agaggagctg atttcacagg cccaagttgt tttacaaggc
      661 aaatccagaa gtgtcattat tcgagaactt cagagaacaa atcttgatgt gaaccttgct
      721 gtaaataatt tacttagccg ggatgatgaa gatggagatg atggggatga tacagccagc
      781 gaatcttatt tgcctggaga ggatcttatg tctctccttg atgccgacat tcattctgcc
      841 cacccaagtg tcattattga tgcagatgcc atgttttctg aagacattag ctattttggt
      901 tacccttctt ttcgtcgttc atcactttcc aggctaggct catctcgagt tctccttctt
      961 ccctta
//
