LOCUS       sequence-81551-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein 
            M, Ehrt S, Schnappinger D
  TITLE     Silencing Mycobacterium smegmatis by using tetracycline repressors.
  JOURNAL   J Bacteriol. 2007 Jul . 189(13):4614-23.
  PUBMED    17483222
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2007 Jul . 189(13):4614-23."
COMMENT     Sequence Label: pUV15tetORm
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     702..721
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tcaccgatcc ggaggaactg gcgcagttcc tctgggggcc gccggacacc gcccccggcg
       61 cctgacgccc cgggccgccc ggcgccgccc ccggcccccg gcggccgcaa aataaaaaag
      121 gggacctcta gggtccccaa ttaattagct aaagcgctag cccatggtca ttaagaccca
      181 ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc gaataagaag
      241 gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata
      301 ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat
      361 acgcaaccta aggtaaaatg ccccacagcg ctgagtgcat ataacgcgtt ctctagtgaa
      421 aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta
      481 ngccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa
      541 cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga
      601 gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcagggcccg cttatttttt
      661 acatgccaat acaatgtagg ctgctctaca ccaagcttct gggcgagttt acgggttgtt
      721 aaaccttcga ttccgacctc attaag
//
