LOCUS       sequence-81556-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein 
            M, Ehrt S, Schnappinger D
  TITLE     Silencing Mycobacterium smegmatis by using tetracycline repressors.
  JOURNAL   J Bacteriol. 2007 Jul . 189(13):4614-23.
  PUBMED    17483222
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2007 Jul . 189(13):4614-23."
COMMENT     Sequence Label: pMC1s
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(693..712)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcaacgcgt gcggccgctg attagctaag cagaaggcca
      241 tcctgacgga tgcctttttc gctttaatac tgcatgcact ctagaaatat tggatcgtcg
      301 gcaccgtcac ggccgtggga ggcggcacga tccgcgacgt gatgatcggc cgcatcccca
      361 cggtgctgcg cagtgagctc tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag
      421 gcccggtgtg agaagggtct ctgacgagcg ggagaaccca cccggggtgg gcgagtttgt
      481 cctgcgtgtg ctcggtcgag taggctctgg gagtacccgt gtgtacgacc agcacggcat
      541 acatcatttc gacgccgaga gattcgccgc ccgaaatgag cacgatccgc atggtaccat
      601 caggaggaat cacttcgcaa tgtctagatt agataaaagt aaagtgatta acagcgcatt
      661 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
      721 tggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggccc tgctcgacgc
      781 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
      841 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
      901 cgatggagca aaagtacatt taggtacacg gcctacagaa aaa
//
