LOCUS       sequence-81558-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transient expression of pcoCas9 gene in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM,
            Sheen J
  TITLE     Multiplex and homologous recombination-mediated genome editing in 
            Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.
  JOURNAL   Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654.
  PUBMED    23929339
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08"; volume: "31"; issue:
            "8"; pages: "688-91"
COMMENT     Sequence Label: HBT-pcoCas9
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(12..264)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(273..320)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gcttatcatc ggatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
       61 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
      121 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
      181 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
      241 ttattgccaa atgtttgaac gatctgcagt cacttcttct tcttagcctg tccagccttc
      301 ttggtagcag ctggtctctt atctcctcca agctgagaaa gatcgattct ggtctcgtaa
      361 agtccggtga tagactggtg gataagggta gcatcaagaa cctccttggt agaggtgtat
      421 ctttttctat cgatggtggt atcgaagtac ttgaaagcag ctggagcacc aaggttggta
      481 agggtgaaaa ggtggatgat gttctcagcc tgctctctga ttggcttatc tctgtgcttg
      541 ttgtaagcag aaagaacctt atcaaggtta gcatcagcaa ggataactct cttagagaac
      601 tcagagattt gctcgatgat ctcatcaagg tagtgcttgt gctgctcaac gaaaagctgc
      661 ttctgctcgt tatcctctgg agatcccttc aacttctcgt agtgagaagc aaggtaaagg
      721 aagttaacgt acttagatgg aagagcaagc tcgtttccct tctgaagctc tccagcagaa
      781 gcaagcattc tctttcttcc gttctcaagc tcgaaaagag agtactttgg caacttgatg
      841 ataagatcct tcttaacctc cttgtatccc ttagcctcaa ggaaatcgat tgggttcttc
      901 tcgaaagaag aacgctccat gatggtgatt ccaagaagct ccttaacaga cttcaacttc
      961 ttagactttc ccttctcaac cttagcaaca aca
//
