LOCUS       sequence-81568-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Link AJ, Phillips D, Church GM
  TITLE     Methods for generating precise deletions and insertions in the 
            genome of wild-type Escherichia coli: application to open reading 
            frame characterization.
  JOURNAL   J Bacteriol. 1997 Oct . 179(20):6228-37.
  PUBMED    9335267
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 1997 Oct . 179(20):6228-37."
COMMENT     Sequence Label: pKOV
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      469..691
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 gggtaagcct gttgatgata ccgctgcctt actgggtgca ttagccagtc tgaatgacct
       61 gtcacgggat aatccgaagt ggtcagactg gaaaatcaga gggcaggaac tgctgaacag
      121 caaaaagtca gatagcacca catagcagac ccgccataaa acgccctgag aagcccgtga
      181 cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt
      241 gccatttacc cccattcact gccagagccg tgagcgcagc ggactgaatg tcacgaaaaa
      301 gacagcgact caggtgccgg atggtcggag acagaaggaa tattcagcga tttgcccgag
      361 cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc tgttttgtag aggagcaaac
      421 agcgtttacg acatcctttt gtaatactgc ggaactgact aaagtagtga gttatacaca
      481 gggctgggat ctattctttt tatctttttt tattctttct ttattctata aattataacc
      541 acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg aatttacaga gggtctagca
      601 gaatttacaa gttttccagc aaaggtctag cagaatttac agatacccac aactcaaagg
      661 aaaaggacta gtaattatca ttgactagcc catctcaatt ggtatagtga ttaaaatcac
      721 ctagaccaat tgagatgtat gtctgaatta gttgttttca aagcaaatga actagcgatt
      781 agtcgctatg acttaacgga gcatgaaacc aagctaattt tatgctgtgt ggcactactc
      841 aaccccacga ttgaaaaccc tacgaggaaa gaacggacgg tatcgttcac ttataaccaa
      901 tacgttcaga tgatgaacat cagtagggaa aatgcttatg gtgtattagc taaagcaacc
      961 agagagctga tgacgagaac tgtgg
//
