LOCUS       sequence-8157-ex        6940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Mayr C, Hemann MT, Bartel DP
  TITLE     Disrupting the pairing between let-7 and Hmga2 enhances oncogenic 
            transformation.
  JOURNAL   Science. 2007 Mar 16. 315(5818):1576-9.
  PUBMED    17322030
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2007 Mar 16. 315(5818):1576-9."
COMMENT     Sequence Label: Hmga2 3'UTR wt luciferase (Luc-wt)
FEATURES             Location/Qualifiers
     source          1..6940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..759
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          829..961
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     944..963
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1006..1024
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1034..1969
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    4895..5016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4906..4925)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4960..4979
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5149..5253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5254..5322,5323..6114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5472..5491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6285..6873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6774..6793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
       61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
      121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
      181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
      241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
      301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
      361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
      421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
      481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
      541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
      601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
      661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
      721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca
      781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
     1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac
     1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta
     1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc
     1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc
     1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga
     1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt
     1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa
     1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg
     1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt
     1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc
     1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac
     1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat
     1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga
     1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac
     1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
     1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
     1981 ctataccggt ctcgatatca ctactagtgt tctagagggg cgccgacatt caatttctac
     2041 ctcagcatca gttggatctt ttgaagggag aagacactgc agtgaccagt tattcttaac
     2101 tgccacggtc tttctacttc ctgcggggtg gggcgggggc ggggctgggc gaggggcggg
     2161 gccggggtgg gcgaaatcgc ataaccttga gaaggactat attaatcact ttgtaatccc
     2221 ttcacagtcc caggtttagt gaaaaactgc tgtaaacacg ggggacacag tttaacaatg
     2281 caacttttaa tgactgtttt ctttttcctt aacttactaa tagtttgtgg atctgataag
     2341 caagggtgtg tggttgaaga aaacctctgt ggtgggctta atcagtcact acatgcaaac
     2401 cctaaaccgg caccctggtg accgggggca ttcgtataag aaaagcattg tgtgtgactc
     2461 tgtgtccact cagatgccac ccccaccatg atcatagaaa atctgcttag gacaccaaag
     2521 atgagaacta gacactactc tcctttcttt gtgtataatc ttgtagacac ttacttgatt
     2581 tttttttctt tttttacttt tcaattctga atgagacaaa atgctggtgt atcttttcat
     2641 acagctagca aaccagaata ggttatgctc gttttttgct ttgttttgtt tttcaaaaag
     2701 ggaagtaaac gagaaccgtt gactcctcca tttatggact catacacagc agcaggagtg
     2761 ataagcccac aagctctctt tcccgcctcg ggaaatctac acagccaaaa gccacttagc
     2821 cataaatgac acttgtcagc cttgaagcat cggagataac tagctgagta aaatgatcct
     2881 gttttggaat ttaatgaaaa ggttaacagt acccaatgaa cccacccaag tgatgacatg
     2941 ggaggagcga aaccgaaatc tcttttgcta tataaaggac actatttttt aaaaaaaaat
     3001 aataaaaaca gctcccgctc tctgtcctct ctccctccct tctctccctc gcctctctct
     3061 cctctctata ttccctgttc ttcattgtgt accagtgtcc gtgaaagacc gcagtaccac
     3121 ttacctcaga tgaagcctgc gtgttacatc ctgtaacacc tttcattttg acataagatg
     3181 gctagccgag gtgcattatc ttggttcgga ctgccatctc tgcattcacg ctgcactttt
     3241 agccagagat gcaataatcc ccactcctca atactacctc tgaatgctac agtgaattta
     3301 cagccctgca cttgttacac gctgctagac acaagccctg caagagaaag gaaaaaaaaa
     3361 agcccaccaa aaccaaacca aaccttactg ggtcggcatc tcagccatcc ccagttctcg
     3421 accattcttc tctgtactct tactccgtct cagcaggcta tgcatgtttc tatgactttt
     3481 ttttttttta attaaaatgt tacaaatgct tgtggcagct ttcctgctag attgttacat
     3541 taatttgaaa cagttttgag tcaagttgct cctaggttct taaggagaat ttttttttca
     3601 gtgacactac tttgtatcac acacacacat ctgtagtgtt caaatataag tctccaagtt
     3661 tgtacctcaa atgaattatt gaaacaaatg gacttcctga tttgcaagga actacctcca
     3721 cacttccaaa ggaacgaact tgcagcctat atcactcatt gatttccttc ccccatgttt
     3781 gaaggagctc aaacctcacc tctccctcat tgaaacattt tttttggtaa aagacacttg
     3841 atagaaacac aattttttta catacttttg caaaaataaa tgaattaaaa tcaagccaac
     3901 cttcaaagaa acttgaaatt ttgctacaac cagctcagcc ttttgcctaa tgcaatgaaa
     3961 aaggaaaaaa atagatttct aagatttgtt gcctagaaga atatgcttga ccgatatttt
     4021 ttcatgtatt ttacacaatg tgatttttgt aaaaaaatgt ctcaagcaga tttgttttgg
     4081 acgcttcttg tgtagagttt ctatgccttt ctctcctatt aagtgtgctg actttccaga
     4141 gtgttaccca ctgggccagg aggtagtttc tcatagtggc ttgtgtcagt ataagttaat
     4201 actgaagcca aatgaaacaa acaaacaacc atgtctcttc cagctgtttt cagggaggtt
     4261 acttcaaagg ccacgtgccg ctctgagact ggcagatggc tcactgttgt gagtcgccaa
     4321 aggagctatg gagagattaa aattcaacat gactgttaac aatgcattaa ataatcaaat
     4381 aaacagtggc ttataaatat cagattctca ttccgggtct tcggatgggc cttacagaaa
     4441 cctcattttg gccagctcat aaaaactgaa gcagcttctc gtgttggcca gactcggcac
     4501 accgagcaat ttccatctct gatgaagtta ttccttattt cctgtatgtt gtacaatcaa
     4561 aacacactac tacctcttaa gtcccagtat acctcatttt tcatactgaa aaaaaagaaa
     4621 aaaaagagaa aaaaaaaggc ttgtgtccaa tggaacagtg agaacatcat aaaattttta
     4681 tatatatagt ttatttttgt gggagataaa ttttataaga ctgtttttta ctgttgttgg
     4741 tcacagctaa gtaagactgg acatctaact tctctaccat ttctgcaagc taagtatgtt
     4801 tgcaggagaa acttatcaag acgattaact gcatccaact ttctccccgt tcctttgtgt
     4861 gccctctgac tgcggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     4921 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     4981 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     5041 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     5101 tgtggtaaaa tcgataagga tccaggtggc acttttcggg gaaatgtgcg cggaacccct
     5161 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5221 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5281 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5341 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5401 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5461 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5521 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5581 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5641 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5701 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5761 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5821 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5881 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5941 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6001 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6061 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6421 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6901 ttcctggcct tttgctggcc ttttgctcac atggctcgac
//
