LOCUS       sequence-81577-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transient expression of pcoCas9 gene in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM,
            Sheen J
  TITLE     Multiplex and homologous recombination-mediated genome editing in 
            Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.
  JOURNAL   Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654.
  PUBMED    23929339
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08"; volume: "31"; issue:
            "8"; pages: "688-91"
COMMENT     Sequence Label: HBT-pcoCas9
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(41..339,340..362,363..550)
                     /label=35SPPDK promoter
                     /note="hybrid promoter consisting of the cauliflower mosaic
                     virus 35S enhancer fused to the maize C4PPDK basal promoter
                     (Yoo et al., 2007)"
     CDS             562..609
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             616..636
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
       61 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
      121 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
      181 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccaca aggagcatcg
      241 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
      301 ctgacgtaag ggatgacgca caatcccact atccttcgcc ccaagcttgg gcccaagctt
      361 gggtcgcgcc ccacggatgg tataagaata aaggcattcc gcgtgcagga ttcacccgtt
      421 cgcctctcac cttttcgctg tactctctcg ccacacacac cccctctcca gctccgttgg
      481 agctccggac agcagcaggc gcggggcggt cacgtagtaa gcagctctcg gctccctctc
      541 cccttgctcc gtggatccat ggattacaag gatgatgatg ataaggatta caaggatgat
      601 gatgataaga tggctccaaa gaagaagaga aaggttggaa tccacggagt tccagctgct
      661 gataagaagt actctatcgg acttgacatc ggaaccaact ctgttggatg ggctgttatc
      721 accgatgagt acaaggttcc atctaagaag ttcaaggttc ttggaaacac cgatagacac
      781 tctatcaaga agaaccttat cggtgctctt cttttcgatt ctggagagac cgctgaggct
      841 accagattga agagaaccgc tagaagaaga tacaccagaa gaaagaacag aatctgctac
      901 cttcaggaaa tcttctctaa cgagatggct aaggttgatg attctttctt ccacagactt
      961 gaggagtctt tccttgttga ggaggataag aagcacgaga gacacccaat cttcggaaac
     1021 atcgttga
//
